北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2014年
3期
53~59
,共null页
糖尿病 运动干预 心肌肌浆网 SERCA2 PLB RyR2
糖尿病 運動榦預 心肌肌漿網 SERCA2 PLB RyR2
당뇨병 운동간예 심기기장망 SERCA2 PLB RyR2
diabetes ; exercise intervention; myocardial sarcoplasmic reticulum; SERCA2 ; PLB ; RyR2
目的:观察运动对糖尿病大鼠心肌肌浆网Ca2+调控蛋白基因表达的影响,探讨运动对心脏的保护作用的分子机制。方法:4月龄雄性sD大鼠,分为安静对照组、安静糖尿病和运动糖尿病组。运动组进行8周耐力训练(20m/min,60min,坡度0,每周5次)。检测心肌肌浆网钙调控蛋白Ca2+ATP酶(SERCA2)、兰尼碱受体II型(RyR2)及受磷蛋白(PLB)mRNA表达水平。结果:安静糖尿病组SERCA2和PLB的mRNA表达上调,RyR2mRNA表达下调,运动使糖尿病组SERCA2和PLB的mRNA表达下调,RyR2mRNA表达上调。结论:8周耐力运动逆转了糖尿病大鼠心肌肌浆网钙调控蛋白基因表达的改变,提示耐力运动具有预防或逆转糖尿病型心肌病变的作用。
目的:觀察運動對糖尿病大鼠心肌肌漿網Ca2+調控蛋白基因錶達的影響,探討運動對心髒的保護作用的分子機製。方法:4月齡雄性sD大鼠,分為安靜對照組、安靜糖尿病和運動糖尿病組。運動組進行8週耐力訓練(20m/min,60min,坡度0,每週5次)。檢測心肌肌漿網鈣調控蛋白Ca2+ATP酶(SERCA2)、蘭尼堿受體II型(RyR2)及受燐蛋白(PLB)mRNA錶達水平。結果:安靜糖尿病組SERCA2和PLB的mRNA錶達上調,RyR2mRNA錶達下調,運動使糖尿病組SERCA2和PLB的mRNA錶達下調,RyR2mRNA錶達上調。結論:8週耐力運動逆轉瞭糖尿病大鼠心肌肌漿網鈣調控蛋白基因錶達的改變,提示耐力運動具有預防或逆轉糖尿病型心肌病變的作用。
목적:관찰운동대당뇨병대서심기기장망Ca2+조공단백기인표체적영향,탐토운동대심장적보호작용적분자궤제。방법:4월령웅성sD대서,분위안정대조조、안정당뇨병화운동당뇨병조。운동조진행8주내력훈련(20m/min,60min,파도0,매주5차)。검측심기기장망개조공단백Ca2+ATP매(SERCA2)、란니감수체II형(RyR2)급수린단백(PLB)mRNA표체수평。결과:안정당뇨병조SERCA2화PLB적mRNA표체상조,RyR2mRNA표체하조,운동사당뇨병조SERCA2화PLB적mRNA표체하조,RyR2mRNA표체상조。결론:8주내력운동역전료당뇨병대서심기기장망개조공단백기인표체적개변,제시내력운동구유예방혹역전당뇨병형심기병변적작용。
The purpose of this study was to explore the influence of exercise on gene expression of cardiac calcium handling protein in diabetic rats, and to detect the molecular mechanism of the protective effect of exercise on heart. Methods: Four-month old Sprague-Dawley rats were divided into three group randomly: sedentary control group (SC), sedentary diabetic group (SD) and exercise diabetic group (ED). The rats in ED group participated in 8 weeks training(20 rn/min, 60min/day, grade 0°, 5 times/week). The mRNA expressions of Ca2+ -ATPase (SER- CA2), type 2 ryanodine receptors (RyR2), and phospholamban (PLB)were measured. Results: The mRNA expressions of SERCA2 and PLB in SD group increased while that of RyR2 decreased. After exercise, the mRNA expressions of SERCA2 and PLB in ED group decreased while that of RyR2 increased. Conclusion : Eight weeks endurance exercise reversed the gene expressions of calcium handling proteins of myocardial sarcoplastic reticulum in diabetic rats, which suggested that endurance training could prevent or therapy the diabetic cardimyopathy.