CAJ | 학술논문

目的：观察大负荷运动后大鼠心肌能量代谢及线粒体变化。方法：4J0只大鼠随机分5组：对照组（C组）、运动后即刻（E0组）、运动后30min组（E30组）、70min组（E70组）、110min组（E110组），每组均为8只；分别测左室心肌ATP、AMP含量、线粒体ST3、ST4和Mirol、Mfn2、Drpl蛋白基因。结果：1）E0组心肌组织ATP浓度略有下降，AMP、AMP／ATP升高；E30组ATP浓度达到最低值（P〈0．01），AMP、AMP／ATP都达到峰值（P〈0．01），E110组基本恢复。2）E0组ST3、ST4、RCR都升高，E30组ST3达到最高值（P〈0．01），E110组基本恢复。3）运动后Mirol、Drp—1的基因和蛋白表达均升高，E0组心肌Mirol表达和蛋白表达都升高到峰值（P〈0．01），随后下降，E110组基本恢复；E30组心肌Drp-1表达和蛋白表达都升高到峰值（P〈0．01），随后下降，E110组与C比较有差异（P〈0．05）；运动后Mfn-2的基因和蛋白表达均下降，E30组Mfn-2表达和蛋白表达都下降到最低值（P〈0．01）。结论：1）-次大负荷运动后ATP含量减少、AMP含量增加，ST3速率加快，RCR增加，随着恢复时程延长心肌能量代谢逐渐改善，运动后110min基本恢复正常；2）-次大负荷运动后心肌线粒体移动增多，融合受抑制趋于分裂；重要的是与移动相关的Mirol表达增加也许是融合分裂发生的先驱因素，使分裂多于合成，进而通过其再次分布做出早期阶段的适宜应答。
목적：관찰대부하운동후대서심기능량대사급선립체변화。방법：4J0지대서수궤분5조：대조조（C조）、운동후즉각（E0조）、운동후30min조（E30조）、70min조（E70조）、110min조（E110조），매조균위8지；분별측좌실심기ATP、AMP함량、선립체ST3、ST4화Mirol、Mfn2、Drpl단백기인。결과：1）E0조심기조직ATP농도략유하강，AMP、AMP／ATP승고；E30조ATP농도체도최저치（P〈0．01），AMP、AMP／ATP도체도봉치（P〈0．01），E110조기본회복。2）E0조ST3、ST4、RCR도승고，E30조ST3체도최고치（P〈0．01），E110조기본회복。3）운동후Mirol、Drp—1적기인화단백표체균승고，E0조심기Mirol표체화단백표체도승고도봉치（P〈0．01），수후하강，E110조기본회복；E30조심기Drp-1표체화단백표체도승고도봉치（P〈0．01），수후하강，E110조여C비교유차이（P〈0．05）；운동후Mfn-2적기인화단백표체균하강，E30조Mfn-2표체화단백표체도하강도최저치（P〈0．01）。결론：1）-차대부하운동후ATP함량감소、AMP함량증가，ST3속솔가쾌，RCR증가，수착회복시정연장심기능량대사축점개선，운동후110min기본회복정상；2）-차대부하운동후심기선립체이동증다，융합수억제추우분렬；중요적시여이동상관적Mirol표체증가야허시융합분렬발생적선구인소，사분렬다우합성，진이통과기재차분포주출조기계단적괄의응답。
Objective： To observe the changes of myocardial energy metabolism, mitochondria morphology and dynamics after an acute heavy load exercise. Methods： Forty male rats were randomly divided into 5 groups（ n = 8 ）： the control group （ C ）, the groups immediately after exercise （ E0）, 30 minutes （ E30）, 70 minutes （E70） and 110 minutes（E110） after exercise respectively. The concentrations of ATP, AMP in left ventricular muscles and the protein and gene expressions of ST3, ST4, Mirol, Mfn2, Drpl in mitochondria were measured. Results ： 1 ） The concentration of ATP decreased slightly and AMP, AMP/ATP increased in E0 group. The concentration of ATP was lowest in E30 group （P 〈0.01 ） while the concentrations of AMP, AMP/ATP were the highest （P 〈0.01 ）. They went back to baseline in E110 group. 2） The protein and gene expressions of ST3, ST4, RCR increased in E0 group, the ST3＇s were highest in E30 group （P 〈0.01 ）, and they were recovered in El10 group. （3） The protein and gene expressions of Mirol and Drpl increased after exercise. Miro 1 increased in E0 group （P 〈 0.01 ） , then it decreased to baseline in E110 group. The protein and gene expressions of Drp - 1 increased to the peak and then decreased, there was significantly different between E110 and C groups （P 〈 0.05 ）. The protein and gene expressions of Mfn - 2 decreased, and were lowest in E30 group （ P 〈 0.01 ）. Conclusion ： 1 ） After an acute heavy load exercise, ATP concentration reduced, AMP concentration raised, and ST3 and RCR increased.Myocardial energy metabolism was improved during the recovery process, and went back to baseline 110 minutes after exercise. 2 ） After an acute heavy load exercise, the movement of myocardial mitochondria increased. Mitochondria＇ fusion was inhibited and mitochondria tended to split. The increase of Mirol＇s expression, which was related to mitochondria movement, may be the important factor for mitochondria＇s fusion and split. It can produce more split than fusion, and mitochondria can response in early stage after its new distribution.