CAJ | 학술논문

对虾白斑综合症病毒（WSSV）编码与核酸合成代谢相关的核糖核苷酸还原酶（Ribonucleotide Reductases，RR），与病毒DNA的复制有关．利用克隆技术将RR基因克隆到L4440载体，构建体内合成dsRNA的大肠杆菌HTn5工程菌．诱导该工程菌合成了RR基因的特异双链RNA（RR—dsRNA）和非特异双链RNA（gfP—dsRNA），分别与WSSV混合共注射凡纳滨对虾，在感染72h后用病毒检测试剂盒提取DNA模板用于荧光定量PCR分析，结果显示RR—dsRNA能有效抑制WSSV病毒粒子的增值．
대하백반종합증병독（WSSV）편마여핵산합성대사상관적핵당핵감산환원매（Ribonucleotide Reductases，RR），여병독DNA적복제유관．이용극륭기술장RR기인극륭도L4440재체，구건체내합성dsRNA적대장간균HTn5공정균．유도해공정균합성료RR기인적특이쌍련RNA（RR—dsRNA）화비특이쌍련RNA（gfP—dsRNA），분별여WSSV혼합공주사범납빈대하，재감염72h후용병독검측시제합제취DNA모판용우형광정량PCR분석，결과현시RR—dsRNA능유효억제WSSV병독입자적증치．
Ribonucleotide reduetases （RR） is an important enzyme during synthesis.In this research, we used RNAi technology to silence RR gene and study the effect of RR gene silence on the WSSV proliferation.We synthesized RR gene specific double-stranded RNA （RR-dsRNA） in vivo by cloning the RR gene into the L4d40 plasmid and inducing dsRNA expression in E.coli HT115.RR-dsRNA was mixed with WSSV and injected into templates were extracted with virus that RR-dsRNA effectively inhibited shrimp Litopenaeus vannamei.72 h post-Infection,WSSV DNA detection kit for quantitative PCR analysis. The results showed WSSV proliferation.