CAJ | 학술논문

目的：探讨有氧运动联合膳食控制对2型糖尿病大鼠血液流变学的影响及其机制。方法：选用6周龄雄性SD大鼠62只，随机抽取8只大鼠作为正常对照组（Control，C组），其余犬鼠在喂饲高脂膳食的基础上腹腔注射小剂量的STZ复制2型糖尿病大鼠模型，并给予有氧运动锻炼或／和膳食控制，13周后测定大鼠的血液流变性以及血糖（FPG）、糖化血清蛋白（Glycated Serum Proteins，GSP）、甘油三酯（TG）和总胆固醇（TC）的含量。结果：①2型糖尿病大鼠模型建立后第13周，与c组相比，DMC组大鼠全血中切粘度、血浆粘度、全血高切还原粘度、红细胞聚集指数、红细胞刚性指数均显著性升高，ESR和红细胞变性指数显著降低。双因素方差分析显示，12周的游泳训练可使2型糖尿病大鼠不同切变率下的全血粘度值均有所降低，其中切变率为1的全血粘度值差畀显著（P〈0．05）；高切还原粘度和低切还原粘度（P〈0．01）和ESR（P=0．05）显著升高；红细胞聚集指数显著降低（P〈0．01），血浆粘度、红细胞压积、红细胞刚性指数、红细胞变形指数都有所改善，但无显著性差异（P〉0．05）。而膳食控制后2型糖尿病大鼠红细胞聚集指数显著降低，其他指标均有所改善，但无显著性差异。有氧运动联合饮食控制对进一步降低2型糖尿病大鼠全血低切粘度、血浆粘度、全血高切、低切还原粘度以及细胞聚集指数，进一步升高ESR和红细胞变形指数，具有显著的交互作用（P〈0．05）。②与C组相比，DMC组FPG、GSP以及TC、TG含量均极显著性升高。双因素方差分析显示，有氧运动能显著降低他DM大鼠FPG、GSP和TC含量，但不能显著降低TG含量。膳食控制能显著降低2型糖尿病大鼠血清GSP、TG和TC含量。有氧运动联合膳食控制对进一步降低2型糖尿病大鼠血清TC含量具有显著的交互作用，但对进一步降低2型糖尿病大鼠FPG、GSP和TG含量无显著的交互作用。结论：①型糖尿病大鼠血液出现高粘滞血症，表现为浓、凝、聚、粘状态。有氧运动可以改善2型糖尿病大鼠的血液流变性，降低血液的高粘滞状态；单纯的膳食控制对改善2型糖尿病大鼠的血液流变性效果不大；在一定程度上有氧运动联合膳食对改善2型糖尿病大鼠的血液流变性效果更大。②糖脂代谢的紊乱是导致糖尿病大鼠高粘滞血症的重要机制，长期的有氧运动和膳食控制可以通过改善糖尿病大鼠的糖脂代谢紊乱改善血液的流变性。目的：探討有氧運動聯閤膳食控製對2型糖尿病大鼠血液流變學的影響及其機製。方法：選用6週齡雄性SD大鼠62隻，隨機抽取8隻大鼠作為正常對照組（Control，C組），其餘犬鼠在餵飼高脂膳食的基礎上腹腔註射小劑量的STZ複製2型糖尿病大鼠模型，併給予有氧運動鍛煉或／和膳食控製，13週後測定大鼠的血液流變性以及血糖（FPG）、糖化血清蛋白（Glycated Serum Proteins，GSP）、甘油三酯（TG）和總膽固醇（TC）的含量。結果：①2型糖尿病大鼠模型建立後第13週，與c組相比，DMC組大鼠全血中切粘度、血漿粘度、全血高切還原粘度、紅細胞聚集指數、紅細胞剛性指數均顯著性升高，ESR和紅細胞變性指數顯著降低。雙因素方差分析顯示，12週的遊泳訓練可使2型糖尿病大鼠不同切變率下的全血粘度值均有所降低，其中切變率為1的全血粘度值差畀顯著（P〈0．05）；高切還原粘度和低切還原粘度（P〈0．01）和ESR（P=0．05）顯著升高；紅細胞聚集指數顯著降低（P〈0．01），血漿粘度、紅細胞壓積、紅細胞剛性指數、紅細胞變形指數都有所改善，但無顯著性差異（P〉0．05）。而膳食控製後2型糖尿病大鼠紅細胞聚集指數顯著降低，其他指標均有所改善，但無顯著性差異。有氧運動聯閤飲食控製對進一步降低2型糖尿病大鼠全血低切粘度、血漿粘度、全血高切、低切還原粘度以及細胞聚集指數，進一步升高ESR和紅細胞變形指數，具有顯著的交互作用（P〈0．05）。②與C組相比，DMC組FPG、GSP以及TC、TG含量均極顯著性升高。雙因素方差分析顯示，有氧運動能顯著降低他DM大鼠FPG、GSP和TC含量，但不能顯著降低TG含量。膳食控製能顯著降低2型糖尿病大鼠血清GSP、TG和TC含量。有氧運動聯閤膳食控製對進一步降低2型糖尿病大鼠血清TC含量具有顯著的交互作用，但對進一步降低2型糖尿病大鼠FPG、GSP和TG含量無顯著的交互作用。結論：①型糖尿病大鼠血液齣現高粘滯血癥，錶現為濃、凝、聚、粘狀態。有氧運動可以改善2型糖尿病大鼠的血液流變性，降低血液的高粘滯狀態；單純的膳食控製對改善2型糖尿病大鼠的血液流變性效果不大；在一定程度上有氧運動聯閤膳食對改善2型糖尿病大鼠的血液流變性效果更大。②糖脂代謝的紊亂是導緻糖尿病大鼠高粘滯血癥的重要機製，長期的有氧運動和膳食控製可以通過改善糖尿病大鼠的糖脂代謝紊亂改善血液的流變性。
목적：탐토유양운동연합선식공제대2형당뇨병대서혈액류변학적영향급기궤제。방법：선용6주령웅성SD대서62지，수궤추취8지대서작위정상대조조（Control，C조），기여견서재위사고지선식적기출상복강주사소제량적STZ복제2형당뇨병대서모형，병급여유양운동단련혹／화선식공제，13주후측정대서적혈액류변성이급혈당（FPG）、당화혈청단백（Glycated Serum Proteins，GSP）、감유삼지（TG）화총담고순（TC）적함량。결과：①2형당뇨병대서모형건립후제13주，여c조상비，DMC조대서전혈중절점도、혈장점도、전혈고절환원점도、홍세포취집지수、홍세포강성지수균현저성승고，ESR화홍세포변성지수현저강저。쌍인소방차분석현시，12주적유영훈련가사2형당뇨병대서불동절변솔하적전혈점도치균유소강저，기중절변솔위1적전혈점도치차비현저（P〈0．05）；고절환원점도화저절환원점도（P〈0．01）화ESR（P=0．05）현저승고；홍세포취집지수현저강저（P〈0．01），혈장점도、홍세포압적、홍세포강성지수、홍세포변형지수도유소개선，단무현저성차이（P〉0．05）。이선식공제후2형당뇨병대서홍세포취집지수현저강저，기타지표균유소개선，단무현저성차이。유양운동연합음식공제대진일보강저2형당뇨병대서전혈저절점도、혈장점도、전혈고절、저절환원점도이급세포취집지수，진일보승고ESR화홍세포변형지수，구유현저적교호작용（P〈0．05）。②여C조상비，DMC조FPG、GSP이급TC、TG함량균겁현저성승고。쌍인소방차분석현시，유양운동능현저강저타DM대서FPG、GSP화TC함량，단불능현저강저TG함량。선식공제능현저강저2형당뇨병대서혈청GSP、TG화TC함량。유양운동연합선식공제대진일보강저2형당뇨병대서혈청TC함량구유현저적교호작용，단대진일보강저2형당뇨병대서FPG、GSP화TG함량무현저적교호작용。결론：①형당뇨병대서혈액출현고점체혈증，표현위농、응、취、점상태。유양운동가이개선2형당뇨병대서적혈액류변성，강저혈액적고점체상태；단순적선식공제대개선2형당뇨병대서적혈액류변성효과불대；재일정정도상유양운동연합선식대개선2형당뇨병대서적혈액류변성효과경대。②당지대사적문란시도치당뇨병대서고점체혈증적중요궤제，장기적유양운동화선식공제가이통과개선당뇨병대서적당지대사문란개선혈액적류변성。
Objective ： To investigate the effects of aerobic exercise combined with dietary control on hemorheology in type 2 diabetic rats and its mechanism. Methods： 8 rats were randomly selected rats as normal control group （Control, C）from 62 6 - week -old male SD rats, the remaining rats were injected intraperitoneally low -dose streptozotocin based on feeding high -fat diet to establish animal models of type 2 diabetes, and given the aerobic exercise and / or dietary control, hemorheological index, blood glucose （ FPG）, glycated serum proteins （ GSP ）, triglyceride （TG） and total cholesterol （ TC ） content were measured after 13 weeks. Results ：（1）After 13 weeks of the establishment of type 2 diabetes model, compared with C group, among the he- morheological indexes in the DM group ,whole blood viscosity in middle shear, plasma viscosity, whole blood reduced viscosity in high shear, erythrocyte aggregation index, erythrocyte rigidity index were significantly higher, ESR and erythrocyte degenera- tion index decreased significantly. Through the double factor variance analysis, 13 weeks of moderate - intensity swimming training could decrease the whole blood viscosity under different shear rate type 2 diabetic rats, in particular whole blood viscos- ity in 1：00 shear had a significant difference（ P 〈 0.05 ） ; Reduced viscosity in high - shear and low shear（ P 〈 0.01 ） and ESR （P = 0.05 ）were significantly increased ;erythrocyte aggregation index decreased significantly（ P 〈 0.01 ）, plasma viscosity, hematocrit,erythrocyte rigidity index, erythrocyte deformability index had some improvement, but there were no significant difference（ P 〉 0.05）. After the dietary control in type 2 diabetes,the erythrocyte aggregation index decreased significantly, other indicators had some improvement, but there were no significant difference. Aerobic exercise combined with diet control could further reduce the whole blood viscosity in low shear, plasma viscosity,whole blood reduced viscosity in high shear or low shear and erythrocyte aggregation index, and further increased ESR and erythrocyte deformability index in type 2 diabetic rats significantly, they had a significant interaction （ P 〈 0.05 ）. （2）Compared with the C group, FPG, GSP, TC and TG content in DM group were extremely significant increase. By two - way ANOVA analysis shows that aerobic exercise could significantly reduce FPG, GSP and TC levels in T2DM rats, but not significantly reduced TG content. Dietary control could significantly reduce serum GSP, TG and TC content in T2DM rats. Aerobic exercise combined with dietary control had a significant interaction on further reducing serum TC content,but no significant interaction on further reducing FPG, GSP and TG content in the T2DM rats. Conclusion ：（1）Hyperviscosity was occurred in type 2 diabetes,which showed concentration, coagulation, accumulation and sticky state. Aerobic exercise could improve hemorheological index in type 2 diabetic rats, significantly lower hyperviscosity. Relying on dietary control simply could not effectively improve hemorheological index in type 2 diabetic rats. Aerobic exercise training combined with dietary control was more effective to improve hemorheological index in type 2 diabetic rats to some extent. （2）Glucose and lipid metabolism disorders was an important mechanism for hyperviscosity, the long - term aerobic exercise and dietary control could improve blood rheology by improving the disorders of the glucose and lipid metabolism in diabetic rats.