CAJ | 학술논문

为探讨运动预适应对溃疡性结肠炎（UC）的影响及其可能机制，将24只C57BL／6小鼠随机分为运动预适应组（Ex组）、模型组（DSS组）和安慰剂对照组（C组），每组8只。Ex组进行8周跑台运动，DSS组和C组安静状态饲养8周。随后Ex组和DSS组自由饮用质量分数为3．5％葡聚糖硫酸钠（DSS）溶液诱导小鼠UC模型，C组自由饮用同等量的蒸馏水，每天观察并记录疾病活动指数（DAI）。7d后处死小鼠，取肠道炎症组织观察病理变化并进行炎症评分；实时荧光定量PCR（RT-qPCR）法检测白细胞介素－1β（IL－1β）和肿瘤坏死因子－α（TNF－α）mRNA表达水平；Westemblot法检测Toll样受体4（TLR4）、总NF－κBp65和核NF．KBp65蛋白表达水平。结果显示，DSS组出现典型的UC临床表现和组织病理学变化，DAI评分、组织炎症评分、IL－1β和TNF－αmRNA表达以及TLR4和核NF—κβp65蛋白表达水平均高于C组（P〈0．01）；Ex组症状和组织病理学变化较DSS组减轻，DAI评分、组织炎症评分、IL－1β和TNF－αmRNA表达以及TLR4和核NF－κβp65蛋白表达水平均低于DSS组（P〈0．01）；3组总NF-κβp65蛋白水平差异无显著性（P〉0．05）。结果表明，长期中等强度运动预适应能够预防UC、减轻UC病情，其机制可能与抑制TLR4-NF-κβ信号通路以及下调下游炎症因子基因表达有关。
위탐토운동예괄응대궤양성결장염（UC）적영향급기가능궤제，장24지C57BL／6소서수궤분위운동예괄응조（Ex조）、모형조（DSS조）화안위제대조조（C조），매조8지。Ex조진행8주포태운동，DSS조화C조안정상태사양8주。수후Ex조화DSS조자유음용질량분수위3．5％포취당류산납（DSS）용액유도소서UC모형，C조자유음용동등량적증류수，매천관찰병기록질병활동지수（DAI）。7d후처사소서，취장도염증조직관찰병리변화병진행염증평분；실시형광정량PCR（RT-qPCR）법검측백세포개소－1β（IL－1β）화종류배사인자－α（TNF－α）mRNA표체수평；Westemblot법검측Toll양수체4（TLR4）、총NF－κBp65화핵NF．KBp65단백표체수평。결과현시，DSS조출현전형적UC림상표현화조직병이학변화，DAI평분、조직염증평분、IL－1β화TNF－αmRNA표체이급TLR4화핵NF—κβp65단백표체수평균고우C조（P〈0．01）；Ex조증상화조직병이학변화교DSS조감경，DAI평분、조직염증평분、IL－1β화TNF－αmRNA표체이급TLR4화핵NF－κβp65단백표체수평균저우DSS조（P〈0．01）；3조총NF-κβp65단백수평차이무현저성（P〉0．05）。결과표명，장기중등강도운동예괄응능구예방UC、감경UC병정，기궤제가능여억제TLR4-NF-κβ신호통로이급하조하유염증인자기인표체유관。
In order to probe into the effects of exercising preadaptation on ulcerative colitis and its possible mecha- nism, the authors divided 24 C57BL/6 mice randomly into an exercising adaptation group （Ex）, a model group （DSS） and a placebo control group （C）, each of which contained 8 mice, let the mice in group Ex exercise on a treadmill for 8 weeks, feed the mice in groups DSS and C in a calm condition for 8 weeks, then let the mice in groups EX and DSS drink a 3.5% dextran sulfate sodium （DSS） solution freely to induce a mouse UC model, let the mice in group C drink the same amount of distilled water freely, observed and recorded disease activity index everyday, and 7 days later, killed the mice, took out inflamed intestinal tissues, observed their pathological changes and gave them an inflammation score, measured interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） mRNA expression levels by means of real-time florescence quantification PCR （RT-qPCR）, as well as Toll-like receptor 4 （TLR4）, total nuclear factor-κβ （NF-κβ） p65 and nuclear NF-κβ p65 protein expression levels by means of Westernblot, and revealed the following findings： the mice in group DSS showed typical UC clinical manifestations and histopathological changes, their DAI score, tissue inflammation score, IL-1β and TNF-α mRNA expression levels as well as TLR4 and nuclear NF-κβ p65 protein expression levels were all higher than those of the mice in group C （P〈0.01）; the symptoms and histopathological changes of the mice in group Ex were alleviated as compared with the mice in group DSS, their DAI score, tissue inflammation score, IL-1β and TNF-α mRNA expression levels as well as TLR4 and nuclear NF-κβ p65 protein expression levels were all lower than those of the mice in group DSS （P〈0.01）; there was no significant difference in total NF-κβ p65 protein expression level between the mice in these three groups. The said findings indicate that long-term medium intensity exercising preadaptation can prevent UC and alleviate UC condition, and that its mechanism may be related to suppressing the signal pathway of TLRs - NF-κβ and down-regulating the gene expression of downstream inflammatory factors.