北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2014年
4期
52~56
,共null页
力竭运动 去甲肾上腺素 5-羟色胺 多巴胺 磷酸肌酸
力竭運動 去甲腎上腺素 5-羥色胺 多巴胺 燐痠肌痠
력갈운동 거갑신상선소 5-간색알 다파알 린산기산
exhaustive exercise ; NE ; 5 - HT ; DA ; phosphocreatine
目的：探讨外源性磷酸肌酸补充对力竭运动时中枢单胺类神经递质代谢的影响。方法：4月龄48只C57／BL小鼠（雌雄不限），1周适应性游泳训练后，随机分为安静对照组（SC）、一次性游泳力竭组（SE）和磷酸肌酸补充游泳力竭组（CE）。磷酸肌酸补充组实验前30min腹腔注射磷酸肌酸钠（500mg／kg），SE组注射生理盐水阴性对照。力竭游泳结束即刻取材皮层、海马、下丘脑、纹状体、脑干和小脑6个脑区。脑组织取材即刻高功率微波灭活糖原合成与分解酶。高效液相色谱法测定各脑区去甲肾上腺素（NE）、5羟色胺（5-HT）及其代谢产物5-羟吲哚乙酸（5-HIAA）、多巴胺（DA）及其代谢产物双羟苯乙酸（DOPAC）含量。结果：1）磷酸肌酸补充明显延长游泳至力竭的时间；2）力竭时皮层、海马和脑干NE和DA增加，下丘脑NE和DA下降，海马和脑干DA代谢增加明显。各脑区5-HT及其代谢力竭时均增加。磷酸肌酸补充对NE和5-HT力竭时变化影响不大，但增加皮层及下丘脑DA含量，使其具有较高的DA／5-HT比值。结论：磷酸肌酸补充延长力竭时间的作用机制之一是调节皮层和下丘脑单胺类神经递质的代谢，使其具有较高的DA／5-HT比值。
目的：探討外源性燐痠肌痠補充對力竭運動時中樞單胺類神經遞質代謝的影響。方法：4月齡48隻C57／BL小鼠（雌雄不限），1週適應性遊泳訓練後，隨機分為安靜對照組（SC）、一次性遊泳力竭組（SE）和燐痠肌痠補充遊泳力竭組（CE）。燐痠肌痠補充組實驗前30min腹腔註射燐痠肌痠鈉（500mg／kg），SE組註射生理鹽水陰性對照。力竭遊泳結束即刻取材皮層、海馬、下丘腦、紋狀體、腦榦和小腦6箇腦區。腦組織取材即刻高功率微波滅活糖原閤成與分解酶。高效液相色譜法測定各腦區去甲腎上腺素（NE）、5羥色胺（5-HT）及其代謝產物5-羥吲哚乙痠（5-HIAA）、多巴胺（DA）及其代謝產物雙羥苯乙痠（DOPAC）含量。結果：1）燐痠肌痠補充明顯延長遊泳至力竭的時間；2）力竭時皮層、海馬和腦榦NE和DA增加，下丘腦NE和DA下降，海馬和腦榦DA代謝增加明顯。各腦區5-HT及其代謝力竭時均增加。燐痠肌痠補充對NE和5-HT力竭時變化影響不大，但增加皮層及下丘腦DA含量，使其具有較高的DA／5-HT比值。結論：燐痠肌痠補充延長力竭時間的作用機製之一是調節皮層和下丘腦單胺類神經遞質的代謝，使其具有較高的DA／5-HT比值。
목적：탐토외원성린산기산보충대력갈운동시중추단알류신경체질대사적영향。방법：4월령48지C57／BL소서（자웅불한），1주괄응성유영훈련후，수궤분위안정대조조（SC）、일차성유영력갈조（SE）화린산기산보충유영력갈조（CE）。린산기산보충조실험전30min복강주사린산기산납（500mg／kg），SE조주사생리염수음성대조。력갈유영결속즉각취재피층、해마、하구뇌、문상체、뇌간화소뇌6개뇌구。뇌조직취재즉각고공솔미파멸활당원합성여분해매。고효액상색보법측정각뇌구거갑신상선소（NE）、5간색알（5-HT）급기대사산물5-간신타을산（5-HIAA）、다파알（DA）급기대사산물쌍간분을산（DOPAC）함량。결과：1）린산기산보충명현연장유영지력갈적시간；2）력갈시피층、해마화뇌간NE화DA증가，하구뇌NE화DA하강，해마화뇌간DA대사증가명현。각뇌구5-HT급기대사력갈시균증가。린산기산보충대NE화5-HT력갈시변화영향불대，단증가피층급하구뇌DA함량，사기구유교고적DA／5-HT비치。결론：린산기산보충연장력갈시간적작용궤제지일시조절피층화하구뇌단알류신경체질적대사，사기구유교고적DA／5-HT비치。
Objective ： This paper explored the effect of phosphocreatine supplement on monoamine neurotransmitter of brain in exhausted exercise mice. Methods： Forty-eight C57/BL mice were randomly divided into the sedentary control group （SC） , exhausted swimming group （SE） and phosphocreatine supplemented exhausted swimming group （CE）. The intraperitoneal injection of phosphocreatine was 30 minutes before swimming in CE group, while the injection of saline solution was in SE group as a placebo. After exhaustive swimming, the cortex, hippocampus, hypothalamus, striatum, brainstem and cerebellum were collected immediately at 0℃ and inactived tissue enzymes by using high-power microwave irradiation. The brain monoamines levels, norepinephrine （NE） , 5 - hydroxytryptamine （5 - HT）, 5 - hydroxyindoleacetie acid （5 - HIAA）, dobaamine （ DA ） , and dihydroxyphenylaeetic acid （DOPAC）, were measured with high-performance liquid chromatography （HPLC）. Results： 1 ） The endurance performance in CE group was significantly prolonged compared with SE group; 2） tn exhaustive group, the concentrations of NE and DA in cerebral cortex, hippocampus and brainstem were significantly increased com- pared with SC group, while NE and DA levels in the hypothalamus were decreased significantly. The level of 5 - HT in all six brain loci were significantly increased in SE group than in SC group. The phosphoereatine supplement had no effect on NE and 5 - HT, but the level of DA in the cerebral cortex, hippocampus and brainstem were significantly increased after phosphoereatine supplement, the ratio of DA/5 - HT in CE group was higherthan in SC group. Conclusion ： The mechanism of prolonged exercise endurance by phosphocreatine supplement is that it can regulate the metabolism of monoamine neurotransmitter in cerebral cortex and hypothalamus and increase DA/5 -HT ratio.
