北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2014年
6期
49~53
,共null页
有氧运动 自发性高血压大鼠 胸主动脉平滑肌 Kv通道
有氧運動 自髮性高血壓大鼠 胸主動脈平滑肌 Kv通道
유양운동 자발성고혈압대서 흉주동맥평활기 Kv통도
aerobic exercise;spontaneously hypertensive rat;thoracic aorta smooth muscle cell;Kv channel
目的：观察有氧运动对自发性高血压大鼠（SHR）离体胸主动脉反应性及电压门控钾通道（Kv）蛋白表达的影响，探讨运动降低血压的血管平滑肌钾通道机制。方法：24只雄性SHR随机平均分为安静组（SHR—SED）和运动组（SHR—EX），另选用同龄雄性WKY大鼠作为正常血压对照组。SHR—EX组进行8周有氧运动，坡度0°，20m/min，60min／d，5d／周。8周后，检验离体胸主动脉对去甲肾上腺素（NE）、4-氨基吡啶（4-AP）的反应性。另用免疫组化DAB法和Western blot法测定胸主动脉平滑肌Kv1．2和Kv1．5的表达情况。结果：1）SHR—EX组血压显著低于SHR-SED组1）SHR-EX组离体胸主动脉对NE的敏感性小于SHR-SED组。2）4-AP诱发的血管收缩反应在SHE—EX组中显著大于SHR．SED组。3）SHR—EX组Kv1．2和Kv1．5表达均显著高于SHE—SED组，有氧运动显著抑制Kv1．2和Kv1．5表达的下调。结论：长期规律的有氧运动训练可降低SHR的收缩压，减弱胸主动脉对缩血管物质的反应性，其重要机制之一为有氧运动显著抑制高血压诱导的平滑肌细胞Kv通道蛋白表达下调。
目的：觀察有氧運動對自髮性高血壓大鼠（SHR）離體胸主動脈反應性及電壓門控鉀通道（Kv）蛋白錶達的影響，探討運動降低血壓的血管平滑肌鉀通道機製。方法：24隻雄性SHR隨機平均分為安靜組（SHR—SED）和運動組（SHR—EX），另選用同齡雄性WKY大鼠作為正常血壓對照組。SHR—EX組進行8週有氧運動，坡度0°，20m/min，60min／d，5d／週。8週後，檢驗離體胸主動脈對去甲腎上腺素（NE）、4-氨基吡啶（4-AP）的反應性。另用免疫組化DAB法和Western blot法測定胸主動脈平滑肌Kv1．2和Kv1．5的錶達情況。結果：1）SHR—EX組血壓顯著低于SHR-SED組1）SHR-EX組離體胸主動脈對NE的敏感性小于SHR-SED組。2）4-AP誘髮的血管收縮反應在SHE—EX組中顯著大于SHR．SED組。3）SHR—EX組Kv1．2和Kv1．5錶達均顯著高于SHE—SED組，有氧運動顯著抑製Kv1．2和Kv1．5錶達的下調。結論：長期規律的有氧運動訓練可降低SHR的收縮壓，減弱胸主動脈對縮血管物質的反應性，其重要機製之一為有氧運動顯著抑製高血壓誘導的平滑肌細胞Kv通道蛋白錶達下調。
목적：관찰유양운동대자발성고혈압대서（SHR）리체흉주동맥반응성급전압문공갑통도（Kv）단백표체적영향，탐토운동강저혈압적혈관평활기갑통도궤제。방법：24지웅성SHR수궤평균분위안정조（SHR—SED）화운동조（SHR—EX），령선용동령웅성WKY대서작위정상혈압대조조。SHR—EX조진행8주유양운동，파도0°，20m/min，60min／d，5d／주。8주후，검험리체흉주동맥대거갑신상선소（NE）、4-안기필정（4-AP）적반응성。령용면역조화DAB법화Western blot법측정흉주동맥평활기Kv1．2화Kv1．5적표체정황。결과：1）SHR—EX조혈압현저저우SHR-SED조1）SHR-EX조리체흉주동맥대NE적민감성소우SHR-SED조。2）4-AP유발적혈관수축반응재SHE—EX조중현저대우SHR．SED조。3）SHR—EX조Kv1．2화Kv1．5표체균현저고우SHE—SED조，유양운동현저억제Kv1．2화Kv1．5표체적하조。결론：장기규률적유양운동훈련가강저SHR적수축압，감약흉주동맥대축혈관물질적반응성，기중요궤제지일위유양운동현저억제고혈압유도적평활기세포Kv통도단백표체하조。
Objectives： This paper aims to investigate the effect of aerobic exercise on the vascular reactivity of thoracic aorta and voltage-gated potassium channel （Kv ） expression in spontaneously hypertensive rats （SHR） ,and to explore the possible potassium channel mechanisms in vascular smooth muscle cells （VSMCs） underlying the beneficial effects of exercise training on hypertension. Methods ： Twenty-four male SHRs were randomly assigned to a sedentary group （SHR-SED） and an exercise training group （SHR-EX）. SHR-EX underwent treadmill training at 20 m/min for 60 min/d ,5 d/wk ,for 8 weeks. Age-matched male WKY rats were used as control. Vascular contractility of thoracic aortas in response to norepinephrine （NE） and 4 -aminopyridine （4 -AP, a selective Kv channel blocker） was measured. Immunohistochemistry and western blotting were performed to examine the expressions of Kv 1.2 and Kv 1.5. Results ： The NE sensitivity of aorta rings was SHR-SED 〉 SHR-EX 〉 WKY. 4 - AP induced a significant increase of vessel tension in all three groups. The maximal tension increased by 4 - AP was SHR-SED 〈 SHR-EX 〈 WKY. Immunohistochemistry and western blotting showed that Kv 1.2 and Kv 1.5 expressions were significantly decreased in hypertension and exercise training attenuated this reduction. Conclusion： Regular aerobic exercise training attenuates SHR-related down-regulation of Kvl. 2 and Kv1. 5 expression in VSMCs,which may be an important mechanism underlying the beneficial effects of exercise training on hypertension and arterial function.
