成都体育学院学报
成都體育學院學報
성도체육학원학보
Journal of Chengdu Physical Education Institute
2014年
6期
62~67
,共null页
林雪灵 李雪 邓文骞 王璐 杨澎湃 张业廷 袁琼嘉
林雪靈 李雪 鄧文鶱 王璐 楊澎湃 張業廷 袁瓊嘉
림설령 리설 산문건 왕로 양팽배 장업정 원경가
有氧运动 衰老 神经细胞粘附分子 额叶 反转录-聚合酶链反应 免疫印迹法 免疫组织化学 大鼠
有氧運動 衰老 神經細胞粘附分子 額葉 反轉錄-聚閤酶鏈反應 免疫印跡法 免疫組織化學 大鼠
유양운동 쇠로 신경세포점부분자 액협 반전록-취합매련반응 면역인적법 면역조직화학 대서
aerobic exercise ; aging; neural cell adhesion molecule ; frontal lobe ; real - time PCR ; Western blotting; immunohistochemistry ; rat
目的：探讨D-半乳糖致衰老的SD大鼠在衰老前和衰老过程中进行有氧运动对其脑衰老和额叶NCAM表达的影响。方法：3月龄雄性SD大鼠60只随机分成运动预处理组（SD组）、衰老组（ND组）、生理盐水对照组（NNa组）、衰老过程中有氧运动干预组（NDs组），n=15。取材后，测定大脑皮质的SOD、GSH—Px活性和MDA含量，Real—time PCR、Westernb lotting检测额叶NCAM基因的表达，免疫组织化学检测其表达部位。结果：ND组大鼠较NNa组出现了嗜睡、食欲不振、行动迟缓、毛色枯黄卷曲并大量脱落等衰老体征；ND组SOD、GSH—Px活性非常显著性低于NNa组（P〈0．01），MDA含量非常显著性多于NNa组（P〈0．01），SD组SOD、GSH—Px活性和MDA含量较ND组均无显著性差异（P〉0．05），NDs组SOD、GSH—Px活性非常显著性高于ND组（P〈O．01），MDA含量非常显著性少于ND组（P〈0．01）；ND组额叶NCAM基因的mRNA和蛋白质表达水平非常显著性低于NNa组（P〈0．01），SD组额叶NCAM基因的mRNA和蛋白质表达水平较ND组无显著性差异（P〉0．05），NDs组额叶NCAM基因的mRNA和蛋白质表达水平非常显著性高于ND组（P〈0．01），额叶NCAM的免疫产物呈棕褐色，阳性表达主要位于额叶颗粒层细胞周缘的神经元胞浆中。结论：D-半乳糖致大鼠脑衰老造模成功；运动预处理对大鼠脑衰老和额叶NCAM的表达均未产生积极作用；衰老过程中进行有氧运动有效地延缓脑衰老，显著上调额叶NCAM的表达。
目的：探討D-半乳糖緻衰老的SD大鼠在衰老前和衰老過程中進行有氧運動對其腦衰老和額葉NCAM錶達的影響。方法：3月齡雄性SD大鼠60隻隨機分成運動預處理組（SD組）、衰老組（ND組）、生理鹽水對照組（NNa組）、衰老過程中有氧運動榦預組（NDs組），n=15。取材後，測定大腦皮質的SOD、GSH—Px活性和MDA含量，Real—time PCR、Westernb lotting檢測額葉NCAM基因的錶達，免疫組織化學檢測其錶達部位。結果：ND組大鼠較NNa組齣現瞭嗜睡、食欲不振、行動遲緩、毛色枯黃捲麯併大量脫落等衰老體徵；ND組SOD、GSH—Px活性非常顯著性低于NNa組（P〈0．01），MDA含量非常顯著性多于NNa組（P〈0．01），SD組SOD、GSH—Px活性和MDA含量較ND組均無顯著性差異（P〉0．05），NDs組SOD、GSH—Px活性非常顯著性高于ND組（P〈O．01），MDA含量非常顯著性少于ND組（P〈0．01）；ND組額葉NCAM基因的mRNA和蛋白質錶達水平非常顯著性低于NNa組（P〈0．01），SD組額葉NCAM基因的mRNA和蛋白質錶達水平較ND組無顯著性差異（P〉0．05），NDs組額葉NCAM基因的mRNA和蛋白質錶達水平非常顯著性高于ND組（P〈0．01），額葉NCAM的免疫產物呈棕褐色，暘性錶達主要位于額葉顆粒層細胞週緣的神經元胞漿中。結論：D-半乳糖緻大鼠腦衰老造模成功；運動預處理對大鼠腦衰老和額葉NCAM的錶達均未產生積極作用；衰老過程中進行有氧運動有效地延緩腦衰老，顯著上調額葉NCAM的錶達。
목적：탐토D-반유당치쇠로적SD대서재쇠로전화쇠로과정중진행유양운동대기뇌쇠로화액협NCAM표체적영향。방법：3월령웅성SD대서60지수궤분성운동예처리조（SD조）、쇠로조（ND조）、생리염수대조조（NNa조）、쇠로과정중유양운동간예조（NDs조），n=15。취재후，측정대뇌피질적SOD、GSH—Px활성화MDA함량，Real—time PCR、Westernb lotting검측액협NCAM기인적표체，면역조직화학검측기표체부위。결과：ND조대서교NNa조출현료기수、식욕불진、행동지완、모색고황권곡병대량탈락등쇠로체정；ND조SOD、GSH—Px활성비상현저성저우NNa조（P〈0．01），MDA함량비상현저성다우NNa조（P〈0．01），SD조SOD、GSH—Px활성화MDA함량교ND조균무현저성차이（P〉0．05），NDs조SOD、GSH—Px활성비상현저성고우ND조（P〈O．01），MDA함량비상현저성소우ND조（P〈0．01）；ND조액협NCAM기인적mRNA화단백질표체수평비상현저성저우NNa조（P〈0．01），SD조액협NCAM기인적mRNA화단백질표체수평교ND조무현저성차이（P〉0．05），NDs조액협NCAM기인적mRNA화단백질표체수평비상현저성고우ND조（P〈0．01），액협NCAM적면역산물정종갈색，양성표체주요위우액협과립층세포주연적신경원포장중。결론：D-반유당치대서뇌쇠로조모성공；운동예처리대대서뇌쇠로화액협NCAM적표체균미산생적겁작용；쇠로과정중진행유양운동유효지연완뇌쇠로，현저상조액협NCAM적표체。
Objective： Aging SD rats induced by injection of D - Galactose were intervened by aerobic exercise ahead of aging and during aging to explore the effect of aerobic exercise on brain aging and expression level of NCAM in frontal lobe. Methods： 60 3 -month -old male SD rats were randomly divided into groups SD （aerobic exercise ahead of aging group）, ND（aging group）, NNa（saline control group） and NDs（ aerobic exercise during aging group）, n = 15. SOD activity, GSH- Px activity and MDA contents in cerebral cortex were determined. Expression level of NCAM in frontal lobe was determined by real -time PCR, western blotting and immunohistochemistry. Results： Group ND, compared with group NNa rats, revealed a lot of obvious aging signs, such as lethargy, loss of appetite, slowness, curly brown hair and shedding; SOD and GSH -Px activity of group ND rats were significantly lower than group NNa（ P 〈 0. 01 ）, MDA content was significantly more than that of group NNa（ P 〈 0.01 ）, SOD and GSH - Px activity and MDA content of group ND rats had no significant difference from group ND （ P 〉 0.05 ） , SOD and GSH - Px activity of group NDs rats were very significantly higher than those of group ND （P 〈 0.01 ） , MDA content was significantly less than that of group ND（ P 〈 0.01 ） ; mRNA and protein expression level of NCAM gene in frontal lobe of group ND rats were significantly lower than those of group NNa（ P 〈 0.01 ）. Compared with group NO, mRNA and protein expression level of NCAM gene in frontal lobe of group SD rats had no significant difference （ P 〉 0.05 ） while mRNA and protein expression level of NCAM gene in frontal lobe of group NDs rats were significantly higher than those of group ND（ P 〈 0.01）.Immunoreactivities of NCAM in frontal lobe were brown, mainly located in neuronal cytoplasm beside the granular cells. Conclusion ： Injection of D - galactose induced brain aging model rats successful; Aerobic exercise ahead of aging did not have a favorable impact on brain aging and aerobic exercise during aging showed an effective impact on delaying brain aging; Aerobic exercise ahead of aging did not have a favorable impact on expression level of NCAM in frontal lobe and aerobic exercise during aging could significantly increase expression level of NCAM in frontal lobe.
