韶关学院学报
韶關學院學報
소관학원학보
Journal of Shaoguan University(Social Science Edition)
2014年
8期
54~57
,共null页
溪黄草 多糖 微波提取 正交试验
溪黃草 多糖 微波提取 正交試驗
계황초 다당 미파제취 정교시험
Rabdosia serra （Maxim） Hara; polysaccharides; microwave; extraction; orthogonal experiment
以多糖提取率为评价指标，采用分光光度法测定溪黄草多糖的含量，在单因素试验的基础上，通过正交优化考察了微波功率、微波时间、料液比对多糖提取率的影响．结果表明，各因素对溪黄草多糖提取率的影响顺序由大到小依次为：微波功率，微波时间，料液比．最佳提取条件为：微波功率为中火，微波时间为25min，料液比为1：15（g／mL）．在此条件下．溪黄草多糖的提取率为15．32％．与传统的索氏提取法相比，微波提取法具有提取效率高、节省时间、操作简便、易工业化等优点．
以多糖提取率為評價指標，採用分光光度法測定溪黃草多糖的含量，在單因素試驗的基礎上，通過正交優化攷察瞭微波功率、微波時間、料液比對多糖提取率的影響．結果錶明，各因素對溪黃草多糖提取率的影響順序由大到小依次為：微波功率，微波時間，料液比．最佳提取條件為：微波功率為中火，微波時間為25min，料液比為1：15（g／mL）．在此條件下．溪黃草多糖的提取率為15．32％．與傳統的索氏提取法相比，微波提取法具有提取效率高、節省時間、操作簡便、易工業化等優點．
이다당제취솔위평개지표，채용분광광도법측정계황초다당적함량，재단인소시험적기출상，통과정교우화고찰료미파공솔、미파시간、료액비대다당제취솔적영향．결과표명，각인소대계황초다당제취솔적영향순서유대도소의차위：미파공솔，미파시간，료액비．최가제취조건위：미파공솔위중화，미파시간위25min，료액비위1：15（g／mL）．재차조건하．계황초다당적제취솔위15．32％．여전통적색씨제취법상비，미파제취법구유제취효솔고、절성시간、조작간편、역공업화등우점．
The optimal extraction technology of polysaccharides from Rabdosia serra （Maxim） Hara was studied by microwave method. The polysaccharides content was determined by visible spectrophotometry method. Based on the single factor experiment, the effects of microwave power, microwave treatment time and the ratio of solid to liquid on polysaccharides extraction efficiency were investigated further by orthogonal tests. The result showed that the effects of each factor on the extraction effects of polysaccharide in order were as follows： ex- traction power〉extraction time〉solid-liquid ratio. And the optimal conditions were microwave power of middle fire for 25 min with solid-liquid ratio 1：20 （g/mL）. Under these conditions, the extraction rate of polysaccharides from Rabdosia serra （Maxim） Hara is 15.32%. Compared to the Soxhlet extraction, Microwave extraction method has some advantages such as more efficient extracting, time saving, easy to control and easy to be industrialized.
