CAJ | 학술논문

目的：研究TGFβ（经典和非经典）通路在运动缓解衰老性肌肉流失（少肌症）中的作用。方法：24只雄性Wistar大鼠分为3组,C组（青年安静组）、S组（40dD-半乳糖注射致衰老组）、E组（40dD-半乳糖注射＋6wk跑台运动的衰老运动组）。检测各组大鼠体重、腓肠肌重量、TGFβ（经典与非经典）通路因子——TGFβ1、MSTN、Phospho-smad2/3、PhosphoMAPKs（p38、JNK1/2、ERK1/2）及通路效应因子——p21、Pax7（WB法）、MyoD mRNA、MyoG mRNA（RT-PCR法）。结果：与C组相比,S组腓肠肌比重（腓肠肌重量/体重）降低,TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs（Phospho-p38、Phospho-JNK1/2、PhosphoERK1/2）、p21、MyoD mRNA、MyoG mRNA升高,Pax7降低;与S组相比,E组腓肠肌比重升高,TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs（Phospho-JNK1/2、Phospho-ERK1/2）降低,p21、Pax7、MyoD mRNA、MyoG mRNA升高。结论：运动训练抑制了TGFβ经典及非经典通路并缓解了衰老肌肉的流失,Pax7、p21、MyoD、MyoG可能作为TGFβ通路的效应器介导了该过程。
목적：연구TGFβ（경전화비경전）통로재운동완해쇠로성기육류실（소기증）중적작용。방법：24지웅성Wistar대서분위3조,C조（청년안정조）、S조（40dD-반유당주사치쇠로조）、E조（40dD-반유당주사＋6wk포태운동적쇠로운동조）。검측각조대서체중、비장기중량、TGFβ（경전여비경전）통로인자——TGFβ1、MSTN、Phospho-smad2/3、PhosphoMAPKs（p38、JNK1/2、ERK1/2）급통로효응인자——p21、Pax7（WB법）、MyoD mRNA、MyoG mRNA（RT-PCR법）。결과：여C조상비,S조비장기비중（비장기중량/체중）강저,TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs（Phospho-p38、Phospho-JNK1/2、PhosphoERK1/2）、p21、MyoD mRNA、MyoG mRNA승고,Pax7강저;여S조상비,E조비장기비중승고,TGFβ1、MSTN、Phospho-smad2/3、Phospho-MAPKs（Phospho-JNK1/2、Phospho-ERK1/2）강저,p21、Pax7、MyoD mRNA、MyoG mRNA승고。결론：운동훈련억제료TGFβ경전급비경전통로병완해료쇠로기육적류실,Pax7、p21、MyoD、MyoG가능작위TGFβ통로적효응기개도료해과정。
Objectvie：To explore the functions of（canonical and uncanonical）TGFβpathway in exercise-induced improvement of sarcopenia.Method：24 male wistar rats were divided into 3groups：C group（quiet young group）,S group（40day D-Glactose injection-induced senecence）,E group（40days D-Glactose injection-induced senecence＋6weeks exercise trainning on treadmill）.To detect body wet weight,gastrocnemius wet weight（by weighment）,TGFβpathway component-TGFβ1,MSTN,Phospho-smad2/3,Phospho-MAPK（p38,JNK1/2,ERK1/2）,TGFβpathway effector-p21,Pax7 by WB and MyoD mRNA,MyoG mRNA by RT-PCR in each group.Result：The ratio of gastrocnemius weight to body weight decreased,but TGFβ1,MSTN,Phospho-smad2,3,Phospho-p38,Phospho-JNK1,2,Phospho-ERK1,2,p21,MyoD mRNA,MyoG mRNA increased and Pax7 decreased in S group vs C group.The ratio of gastrocnemius weight to body weight increased,but TGFβ1,MSTN,Phospho-smad2,3,Phospho-JNK1,2,Phospho-ERK1,2decreased,Phospho-p38 unchanged,and p21,Pax7,MyoD mRNA,MyoG mRNA increased in E group vs S group.Conclusion：Exericse trainning can improve senescene-induced muscle loss by inhibiting TGFβcanonical and uncanonical pathway with the mediation of Pax7,p21,MyoD and MyoG.