成都体育学院学报
成都體育學院學報
성도체육학원학보
Journal of Chengdu Physical Education Institute
2014年
10期
60~63
,共null页
龚晓明 郑澜 瞿树林 雷志军 凡婷 陈伊琳
龔曉明 鄭瀾 瞿樹林 雷誌軍 凡婷 陳伊琳
공효명 정란 구수림 뢰지군 범정 진이림
有氧运动 内皮祖细胞 骨髓 细胞培养 增殖
有氧運動 內皮祖細胞 骨髓 細胞培養 增殖
유양운동 내피조세포 골수 세포배양 증식
aerobic exercise：endothelial progenitor cells：bone marrow;cell culture;proliferation
目的：探讨有氧运动对中老年大鼠骨髓来源内皮祖细胞增殖能力的影响。方法：健康中年雄性SD大鼠12只，按体重随机分为两组，即空白对照组和有氧运动组。采用乳酸阈测定法和递增负荷跑台训练建立有氧运动模型，每周训练6天，为期16周。方案结束后12h提取大鼠骨髓，运用密度梯度离心法分离得到单个核细胞，以EGM-2培养诱导其分化获取内皮祖细胞，通过双荧光染色法观察细胞对乙酰化低密度脂蛋白的摄取和与荆豆凝集素1的结合，并结合形态学特征鉴定内皮祖细胞。倒置显微镜下计数贴壁细胞数量和计算集落形成率以反映细胞增殖情况。结果：各组贴壁细胞数量第7d多于第48h（P〈0.01）；与对照组相比，有氧运动组贴壁细胞数量明显增加（P〈0.01），且集落形成率大（P〈0.01）。结论：有氧运动能促进中老年大鼠骨髓来源的内皮祖细胞增殖。
目的：探討有氧運動對中老年大鼠骨髓來源內皮祖細胞增殖能力的影響。方法：健康中年雄性SD大鼠12隻，按體重隨機分為兩組，即空白對照組和有氧運動組。採用乳痠閾測定法和遞增負荷跑檯訓練建立有氧運動模型，每週訓練6天，為期16週。方案結束後12h提取大鼠骨髓，運用密度梯度離心法分離得到單箇覈細胞，以EGM-2培養誘導其分化穫取內皮祖細胞，通過雙熒光染色法觀察細胞對乙酰化低密度脂蛋白的攝取和與荊豆凝集素1的結閤，併結閤形態學特徵鑒定內皮祖細胞。倒置顯微鏡下計數貼壁細胞數量和計算集落形成率以反映細胞增殖情況。結果：各組貼壁細胞數量第7d多于第48h（P〈0.01）；與對照組相比，有氧運動組貼壁細胞數量明顯增加（P〈0.01），且集落形成率大（P〈0.01）。結論：有氧運動能促進中老年大鼠骨髓來源的內皮祖細胞增殖。
목적：탐토유양운동대중노년대서골수래원내피조세포증식능력적영향。방법：건강중년웅성SD대서12지，안체중수궤분위량조，즉공백대조조화유양운동조。채용유산역측정법화체증부하포태훈련건립유양운동모형，매주훈련6천，위기16주。방안결속후12h제취대서골수，운용밀도제도리심법분리득도단개핵세포，이EGM-2배양유도기분화획취내피조세포，통과쌍형광염색법관찰세포대을선화저밀도지단백적섭취화여형두응집소1적결합，병결합형태학특정감정내피조세포。도치현미경하계수첩벽세포수량화계산집락형성솔이반영세포증식정황。결과：각조첩벽세포수량제7d다우제48h（P〈0.01）；여대조조상비，유양운동조첩벽세포수량명현증가（P〈0.01），차집락형성솔대（P〈0.01）。결론：유양운동능촉진중노년대서골수래원적내피조세포증식。
Objective： The paper is to explore the effects of aerobics on proliferative capacity in endothelial progeni-tor cells from bone marrow of old male rats. Method： 12 old male Sprague - Dawley rats are randomly and averagely di-vided into 2 groups, the control group and the aerobics group. The aerobic exercise mode is established by using the methods of lactic acid threshold and incremental treadmill exercise. Mononuclear cells from bone marrow are obtained by density gradient centrifugation 12 hours after the scheme has ended up. The cells are suspended in conditioned medium EGM - 2 for culturing in vitro. Their phenotypes are confirmed by uptake of acetylated LDL and binding of fluorescein isothiocyanate （FITC） - labeled Ulex europaeus agglutinin 1 （UEA - 1） lectin. Inverted microscope observation is a-dopted to understand the morphological changes of endothelial progenitor cells and to calculate cells count and colony forming efficiency, so that the proliferation situation of endothelial progenitor cells is obtained. Results： Adherent cells increase more obviously than group control both at 48h and day 7（P 〈 0. 01）. The number of adherent cells and colony forming efficiency in aerobics group is more than that in the control groups（P 〈 0. 01）. Conclusion： Proliferative capaci-ty of endothelial progenitor cells from bone marrow in old male rats can be developed by aerobic exercise.
