石家庄学院学报
石傢莊學院學報
석가장학원학보
Journal of Shijiazhuang University
2014年
6期
28~30
,共null页
任蕾 冯海燕 杨晓辉 雷霓
任蕾 馮海燕 楊曉輝 雷霓
임뢰 풍해연 양효휘 뢰예
柿蒂 三萜酸 含量测定 分光光度法
柿蒂 三萜痠 含量測定 分光光度法
시체 삼첩산 함량측정 분광광도법
Kaki calyx; Triterpenoidic acid; content determination
测定中药柿蒂中总三萜酸的含量,以药材中指标性成分熊果酸为对照品,采用5%香草醛-冰醋酸、高氯酸显色后,分光光度法测定柿蒂提取液中总三萜酸的含量.结果显示：三萜酸含量在2.32-16.24μg/mL范围内呈良好线性关系,其回归方程为：A=0.056 7C＋0.000 286,r=0.998 5.柿蒂中总三萜酸含量为5.93%,加样回收率平均为103.09%.该法操作简便,重现性好,结果可靠,可用于柿蒂药材中三萜酸成分的质量控制。
測定中藥柿蒂中總三萜痠的含量,以藥材中指標性成分熊果痠為對照品,採用5%香草醛-冰醋痠、高氯痠顯色後,分光光度法測定柿蒂提取液中總三萜痠的含量.結果顯示：三萜痠含量在2.32-16.24μg/mL範圍內呈良好線性關繫,其迴歸方程為：A=0.056 7C＋0.000 286,r=0.998 5.柿蒂中總三萜痠含量為5.93%,加樣迴收率平均為103.09%.該法操作簡便,重現性好,結果可靠,可用于柿蒂藥材中三萜痠成分的質量控製。
측정중약시체중총삼첩산적함량,이약재중지표성성분웅과산위대조품,채용5%향초철-빙작산、고록산현색후,분광광도법측정시체제취액중총삼첩산적함량.결과현시：삼첩산함량재2.32-16.24μg/mL범위내정량호선성관계,기회귀방정위：A=0.056 7C＋0.000 286,r=0.998 5.시체중총삼첩산함량위5.93%,가양회수솔평균위103.09%.해법조작간편,중현성호,결과가고,가용우시체약재중삼첩산성분적질량공제。
The content of triterpenoidic acid in Kaki calyx is determined. With ursolic acid as the reference,5%vanillin-acetic acid and perchloric acid as the developer,the spectrophotometric method is used for determining the triterpenoidic acid in Kaki calyx. Results show that the calibration curve is linear（r=0.9985） in the range of 2.32~16.24 μg/mL,and that the regression equation is A=0.0567C＋0.000286. The content of triterpenoidic acid in Kaki calyx is about 5. 93% and the average recovery is 103.09%. Therefore,the method is simple,accurate and reproducible,and it could be used for monitoring the quality of triterpenoidic acid in Kaki calyx.
