天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2014年
3期
193~196
,共null页
小胶质细胞 运动疲劳 海马 炎症反应 学习记忆功能
小膠質細胞 運動疲勞 海馬 炎癥反應 學習記憶功能
소효질세포 운동피로 해마 염증반응 학습기억공능
microglia;exercise-induced fatigue;hippocampus;inflammation;the function of learning and memory
目的：观察反复力竭运动对大鼠海马小胶质细胞形态及炎症因子白介素-1β（interleukin 1 beta,简称IL-1β）、肿瘤坏死因子（tumor necrosisfatctor-alpha,简称TNF-α）和诱导型一氧化氮合酶（inducer type Nitric-Oxide Synthase,简称iNOS）表达的影响。方法：雄性SD大鼠随机分为对照组（CG）和运动疲劳组（EG）,CG大鼠常规饲养不进行跑台运动,EG大鼠进行反复7天递增负荷的力竭跑台运动。采用免疫组化方法观察大鼠海马小胶质细胞形态和激活水平,通过半定量PCR技术检测海马组织IL-1β、TNF-α及iNOS mRNA的表达,并使用Y迷宫主动回避行为学检测学习和记忆能力。结果：与CG相比,EG大鼠海马小胶质细胞体积较大、胞体变圆、突起增多并且染色变深,统计学分析阳性细胞面积和染色消减灰度值均显著高于CG（P〈0.05,P〈0.01）,IL-1β、TNF-α及iNOSmRNA的表达水平也显著性高于CG（P〈0.01）,并且EG大鼠学习记忆能力较CG大鼠显下降（P〈0.05,P〈0.01）。结论：反复力竭运动后,大鼠海马小胶质细胞处于激活状态,海马内炎症因子释放增多,并伴随有学习记忆能力的下降。提示：运动疲劳所致海马小胶质细胞的大量激活可能是引发神经炎症反应,进而降低大鼠学习记忆能力的重要因素之一。
目的：觀察反複力竭運動對大鼠海馬小膠質細胞形態及炎癥因子白介素-1β（interleukin 1 beta,簡稱IL-1β）、腫瘤壞死因子（tumor necrosisfatctor-alpha,簡稱TNF-α）和誘導型一氧化氮閤酶（inducer type Nitric-Oxide Synthase,簡稱iNOS）錶達的影響。方法：雄性SD大鼠隨機分為對照組（CG）和運動疲勞組（EG）,CG大鼠常規飼養不進行跑檯運動,EG大鼠進行反複7天遞增負荷的力竭跑檯運動。採用免疫組化方法觀察大鼠海馬小膠質細胞形態和激活水平,通過半定量PCR技術檢測海馬組織IL-1β、TNF-α及iNOS mRNA的錶達,併使用Y迷宮主動迴避行為學檢測學習和記憶能力。結果：與CG相比,EG大鼠海馬小膠質細胞體積較大、胞體變圓、突起增多併且染色變深,統計學分析暘性細胞麵積和染色消減灰度值均顯著高于CG（P〈0.05,P〈0.01）,IL-1β、TNF-α及iNOSmRNA的錶達水平也顯著性高于CG（P〈0.01）,併且EG大鼠學習記憶能力較CG大鼠顯下降（P〈0.05,P〈0.01）。結論：反複力竭運動後,大鼠海馬小膠質細胞處于激活狀態,海馬內炎癥因子釋放增多,併伴隨有學習記憶能力的下降。提示：運動疲勞所緻海馬小膠質細胞的大量激活可能是引髮神經炎癥反應,進而降低大鼠學習記憶能力的重要因素之一。
목적：관찰반복력갈운동대대서해마소효질세포형태급염증인자백개소-1β（interleukin 1 beta,간칭IL-1β）、종류배사인자（tumor necrosisfatctor-alpha,간칭TNF-α）화유도형일양화담합매（inducer type Nitric-Oxide Synthase,간칭iNOS）표체적영향。방법：웅성SD대서수궤분위대조조（CG）화운동피로조（EG）,CG대서상규사양불진행포태운동,EG대서진행반복7천체증부하적력갈포태운동。채용면역조화방법관찰대서해마소효질세포형태화격활수평,통과반정량PCR기술검측해마조직IL-1β、TNF-α급iNOS mRNA적표체,병사용Y미궁주동회피행위학검측학습화기억능력。결과：여CG상비,EG대서해마소효질세포체적교대、포체변원、돌기증다병차염색변심,통계학분석양성세포면적화염색소감회도치균현저고우CG（P〈0.05,P〈0.01）,IL-1β、TNF-α급iNOSmRNA적표체수평야현저성고우CG（P〈0.01）,병차EG대서학습기억능력교CG대서현하강（P〈0.05,P〈0.01）。결론：반복력갈운동후,대서해마소효질세포처우격활상태,해마내염증인자석방증다,병반수유학습기억능력적하강。제시：운동피로소치해마소효질세포적대량격활가능시인발신경염증반응,진이강저대서학습기억능력적중요인소지일。
Objective：To observe the effects of microglia morphology and expression of inflammation factors include interleukin 1 beta（IL-1β）,tumor necrosis factor-alpha（TNF-α）and inducer type Nitric-Oxide Syntheses（iNOS）in SD rat hippocampus after repeated exhaustive exercise. Methods：Male SD rats were randomly divided into control group（CG）and exercise-induced fatigue group（EG）. Seven days of increasing load exercise to build exercise-induced fatigue model,CG group was not intervention. The immunohistochemistry were used to observe the morphological and the activation level of microglia. The expression of IL-1β,TNF-α and iNOS mRNA in hippocampus was detected with semi-quantitative RT-PCR,and the Y maze active avoidance behavior were used to detect the learning and memory ability. Results：Compared with CG group,the volume of microglia cells was larger,the soma became round,the protuberance was increased and the stained was darker. Statistical analysis that the area of positive cells and the staining gray level were significantly higher than in control group（P〈0.05,P〈0.01）,while the expression of IL-1β,TNF-α and iNOS mRNA was also significantly higher than the control group（P〈0.01）.And the learning and memory ability of rats in exercise-induced fatigue group was significantly lower than the control group（P〈0.05,P〈0.01）. Conclusions：After repeated exhaustive exercise,the microglia cells were activated,inflammation factors in rat hippocampus were increased and the learning and memory ability of rat was declined. The results indicate that the microglia activation caused by exercise-induced fatigue may induce neuroinflammation and it might be one of the important factors leading to the decline of learning and memory ability in rats.
