体育科学
體育科學
체육과학
China Sport Science
2014年
9期
31~38
,共null页
刘泽广 潘珊珊 郝喆 陆矫 苑建齐
劉澤廣 潘珊珊 郝喆 陸矯 苑建齊
류택엄 반산산 학철 륙교 원건제
B型钠尿肽 运动预适应 心肌保护效应 心肌损伤 大鼠
B型鈉尿肽 運動預適應 心肌保護效應 心肌損傷 大鼠
B형납뇨태 운동예괄응 심기보호효응 심기손상 대서
B-type natriuretic peptide; exercise preconditioning; cardioprotection; myocardial injury; rat
目的：探讨运动预适应（EP）对心肌BNP表达变化的影响以及在运动预适应保护效应期间BNP是否发挥了保护效应。方法：SD大鼠分为对照组（C组）、大强度运动组（HE组）、早期运动预适应组（EEP组）、晚期运动预适应组（LEP组）、早期运动预适应＋大强度运动组（EEP＋HE组）、晚期运动预适应＋大强度运动组（LEP＋HE组）。在EP动物模型基础上,通过大强度运动致大鼠运动性心肌损伤。用免疫化学发光法检测血浆cTnI含量,HE染色、HBFP染色法和变色酸2R-亮绿染色法检测心肌形态及缺血缺氧改变。分别通过免疫组织化学法和酶联免疫吸附法检测心肌组织和血浆中的BNP表达变化。结果：与C组相比,HE组cTnI和缺血缺氧水平显著升高,而EEP组和LEP组无显著差异;HE组、EEP组和LEP组心肌组织BNP均呈显著性升高,而血浆BNP仅HE组和EEP组有显著性升高,LEP组却无明显差异。与HE组相比,EEP＋HE组和LEP＋HE组cTnI和缺血缺氧水平明显下降,心肌组织BNP均呈显著性升高,而血浆BNP仅LEP＋HE组有显著升高,EEP＋HE组无显著性差异。结论：EP可有效促进心肌分泌BNP,且BNP参与了EP对运动性心肌损伤的保护效应。
目的：探討運動預適應（EP）對心肌BNP錶達變化的影響以及在運動預適應保護效應期間BNP是否髮揮瞭保護效應。方法：SD大鼠分為對照組（C組）、大彊度運動組（HE組）、早期運動預適應組（EEP組）、晚期運動預適應組（LEP組）、早期運動預適應＋大彊度運動組（EEP＋HE組）、晚期運動預適應＋大彊度運動組（LEP＋HE組）。在EP動物模型基礎上,通過大彊度運動緻大鼠運動性心肌損傷。用免疫化學髮光法檢測血漿cTnI含量,HE染色、HBFP染色法和變色痠2R-亮綠染色法檢測心肌形態及缺血缺氧改變。分彆通過免疫組織化學法和酶聯免疫吸附法檢測心肌組織和血漿中的BNP錶達變化。結果：與C組相比,HE組cTnI和缺血缺氧水平顯著升高,而EEP組和LEP組無顯著差異;HE組、EEP組和LEP組心肌組織BNP均呈顯著性升高,而血漿BNP僅HE組和EEP組有顯著性升高,LEP組卻無明顯差異。與HE組相比,EEP＋HE組和LEP＋HE組cTnI和缺血缺氧水平明顯下降,心肌組織BNP均呈顯著性升高,而血漿BNP僅LEP＋HE組有顯著升高,EEP＋HE組無顯著性差異。結論：EP可有效促進心肌分泌BNP,且BNP參與瞭EP對運動性心肌損傷的保護效應。
목적：탐토운동예괄응（EP）대심기BNP표체변화적영향이급재운동예괄응보호효응기간BNP시부발휘료보호효응。방법：SD대서분위대조조（C조）、대강도운동조（HE조）、조기운동예괄응조（EEP조）、만기운동예괄응조（LEP조）、조기운동예괄응＋대강도운동조（EEP＋HE조）、만기운동예괄응＋대강도운동조（LEP＋HE조）。재EP동물모형기출상,통과대강도운동치대서운동성심기손상。용면역화학발광법검측혈장cTnI함량,HE염색、HBFP염색법화변색산2R-량록염색법검측심기형태급결혈결양개변。분별통과면역조직화학법화매련면역흡부법검측심기조직화혈장중적BNP표체변화。결과：여C조상비,HE조cTnI화결혈결양수평현저승고,이EEP조화LEP조무현저차이;HE조、EEP조화LEP조심기조직BNP균정현저성승고,이혈장BNP부HE조화EEP조유현저성승고,LEP조각무명현차이。여HE조상비,EEP＋HE조화LEP＋HE조cTnI화결혈결양수평명현하강,심기조직BNP균정현저성승고,이혈장BNP부LEP＋HE조유현저승고,EEP＋HE조무현저성차이。결론：EP가유효촉진심기분비BNP,차BNP삼여료EP대운동성심기손상적보호효응。
Objective：To view the change of B-type natriuretic peptide（BNP）expression during exercise preconditioning and determine whether BNP participated incardioprotective effect of exercise preconditioning.Methods：SD rats were divided into control group（C）,HE group,EEP group,LEP group,EEP＋HE group,and LEP＋HE group.After establishing of EP animal model,high-intensity exercise on treadmill for inducing myocardial injury.Then the lever of cTnI was detected by chemiluminescent immunoassay.HE staining was used to observe the changes in ventricular structure.Hematoxylin-basic-fuchsin-picric acid（HBFP）staining and Chromotrope 2R-brilliant green（C-2R）staining were evaluated the degree of myocardial ischemia/hypoxia.The change of BNP in myocardium and plasma were detected by immunohistoChemistry and enzyme inked immunosorbent assay respectively.Results：As compared with the group C,there was no significant difference in the level of cTnI and the degree of myocardial ischemia/hypoxia,but it has a significant increase in group HE.Compared with the group C,the expression of BNP in myocardium was increased significantly in group HE,group EEP and group LEP,but the level of BNP in plasma significantly increase only in group HE and group EEP,group LEP has no significant difference.As compared with group HE,there was a significant decrease in the level of cTnI and the degree of myocardial ischemia/hypoxia in group EEP＋HE and group LEP＋HE.Compared with group HE,the expression of BNP in myocardium was increased significantly in group EEP＋HE and group LEP＋HE,but the level of BNP in plasma significantly increase only in group LEP＋HE,group EEP＋HE has no significant difference.Conclusion：exercise preconditioning can induce myocardium to produce BNP,and BNP participated in cardioprotective effect of exercise preconditioning.
