体育科学
體育科學
체육과학
China Sport Science
2014年
9期
39~43
,共null页
胡玉龙 徐慧 王永梅 昝销 李宁川
鬍玉龍 徐慧 王永梅 昝銷 李寧川
호옥룡 서혜 왕영매 잠소 리저천
心肌肥大 运动 能量代谢 AMPK PPAR-α
心肌肥大 運動 能量代謝 AMPK PPAR-α
심기비대 운동 능량대사 AMPK PPAR-α
cardiac hypertrophy; exercise; energy metabolism; AMPK; PPAR-α
目的：探讨运动性和病理性心肌肥大模型在能量代谢方面的差异及其调控因素。方法：采用游泳训练构建运动性心肌肥大模型,用主动脉弓缩窄术（TAC）构建病理性心肌肥大模型。12周龄雄性C57Bl/6J小鼠随机分为4组：对照组（Control）,运动组（Swim）,假手术组（Sham）,手术组（TAC）,每组10只。实验结束,采用二维超声心动图检测小鼠心室壁肥厚程度和心功能;对心肌组织切片进行Masson染色检测心肌组织纤维化程度;比色法检测心肌组织中游离脂肪酸（FFA）和葡萄糖（Glu）含量;应用实时定量PCR（RT-PCR）检测心肌组织AMPK和PPAR-αmRNA的水平。结果：1）游泳运动和TAC手术两种方法均能使小鼠产生明显的心肌肥大。与相应的对照组相比,两种肥大模型的心脏室间隔厚度（IVS）和左室后壁厚度（LVPW）之和均明显升高（P〈0.05）,心重/体重比值,左心室/胫骨长度比值与相应的对照组相比也都明显增加（P〈0.05）;TAC组ANP、BNP的mRNA水平比Swim组明显升高（P〈0.01）,心肌组织纤维化程度比Swim组明显加重。2）与Sham组相比,TAC组心肌中FFA含量明显增高（P〈0.05）,且显著高于Swim组（P〈0.01）;而Swim组心肌的Glu含量比Control组明显升高（P〈0.05）,且高于TAC组（P〈0.05）。3）与相应的对照组相比,Swim组AMPK mRNA表达水平明显升高（P〈0.05）,且显著高于TAC组（P〈0.01）;TAC组PPAR-αmRNA表达水平明显降低（P〈0.05）,也明显低于Swim组（P〈0.05）。结论：运动能改善肥大心肌的能量代谢,减小＂能量代谢胚胎型再演＂的程度,其机制可能是通过激活AMPK的表达来进行调控的。
目的：探討運動性和病理性心肌肥大模型在能量代謝方麵的差異及其調控因素。方法：採用遊泳訓練構建運動性心肌肥大模型,用主動脈弓縮窄術（TAC）構建病理性心肌肥大模型。12週齡雄性C57Bl/6J小鼠隨機分為4組：對照組（Control）,運動組（Swim）,假手術組（Sham）,手術組（TAC）,每組10隻。實驗結束,採用二維超聲心動圖檢測小鼠心室壁肥厚程度和心功能;對心肌組織切片進行Masson染色檢測心肌組織纖維化程度;比色法檢測心肌組織中遊離脂肪痠（FFA）和葡萄糖（Glu）含量;應用實時定量PCR（RT-PCR）檢測心肌組織AMPK和PPAR-αmRNA的水平。結果：1）遊泳運動和TAC手術兩種方法均能使小鼠產生明顯的心肌肥大。與相應的對照組相比,兩種肥大模型的心髒室間隔厚度（IVS）和左室後壁厚度（LVPW）之和均明顯升高（P〈0.05）,心重/體重比值,左心室/脛骨長度比值與相應的對照組相比也都明顯增加（P〈0.05）;TAC組ANP、BNP的mRNA水平比Swim組明顯升高（P〈0.01）,心肌組織纖維化程度比Swim組明顯加重。2）與Sham組相比,TAC組心肌中FFA含量明顯增高（P〈0.05）,且顯著高于Swim組（P〈0.01）;而Swim組心肌的Glu含量比Control組明顯升高（P〈0.05）,且高于TAC組（P〈0.05）。3）與相應的對照組相比,Swim組AMPK mRNA錶達水平明顯升高（P〈0.05）,且顯著高于TAC組（P〈0.01）;TAC組PPAR-αmRNA錶達水平明顯降低（P〈0.05）,也明顯低于Swim組（P〈0.05）。結論：運動能改善肥大心肌的能量代謝,減小＂能量代謝胚胎型再縯＂的程度,其機製可能是通過激活AMPK的錶達來進行調控的。
목적：탐토운동성화병이성심기비대모형재능량대사방면적차이급기조공인소。방법：채용유영훈련구건운동성심기비대모형,용주동맥궁축착술（TAC）구건병이성심기비대모형。12주령웅성C57Bl/6J소서수궤분위4조：대조조（Control）,운동조（Swim）,가수술조（Sham）,수술조（TAC）,매조10지。실험결속,채용이유초성심동도검측소서심실벽비후정도화심공능;대심기조직절편진행Masson염색검측심기조직섬유화정도;비색법검측심기조직중유리지방산（FFA）화포도당（Glu）함량;응용실시정량PCR（RT-PCR）검측심기조직AMPK화PPAR-αmRNA적수평。결과：1）유영운동화TAC수술량충방법균능사소서산생명현적심기비대。여상응적대조조상비,량충비대모형적심장실간격후도（IVS）화좌실후벽후도（LVPW）지화균명현승고（P〈0.05）,심중/체중비치,좌심실/경골장도비치여상응적대조조상비야도명현증가（P〈0.05）;TAC조ANP、BNP적mRNA수평비Swim조명현승고（P〈0.01）,심기조직섬유화정도비Swim조명현가중。2）여Sham조상비,TAC조심기중FFA함량명현증고（P〈0.05）,차현저고우Swim조（P〈0.01）;이Swim조심기적Glu함량비Control조명현승고（P〈0.05）,차고우TAC조（P〈0.05）。3）여상응적대조조상비,Swim조AMPK mRNA표체수평명현승고（P〈0.05）,차현저고우TAC조（P〈0.01）;TAC조PPAR-αmRNA표체수평명현강저（P〈0.05）,야명현저우Swim조（P〈0.05）。결론：운동능개선비대심기적능량대사,감소＂능량대사배태형재연＂적정도,기궤제가능시통과격활AMPK적표체래진행조공적。
To explore the differences of energy metabolism in exercise-induced and pathological cardiac hypertrophy and its regulation factor.Methods：The exercise-induced cardiac hypertrophy model was made by swim training,and the pathological model was made by transverse aortic constriction（TAC）.12-week-old C57Bl/6J mice were divided randomly into control group,swim group,sham group and TAC group.By the end of experiments,cardiac hypertrophy,contractility were evaluated by echocardiography,and myocardial fibrosis were detected by Masson staining.Myocardial free fatty acid（FFA）and glucose levels were measured by colorimetric detection,AMPK and PPAR-αmRNA expression were detected by real time PCR（RTPCR）.Results：1）Significant cardiac hypertrophy could both produced by swim training and TAC in mice.Compared with corresponding control groups,interventricular septum thickness（IVS）and left ventricular posterior wall thickness（LVPW）of two cardiac hypertrophy models were significantly increased（P〈0.05）,heart weight/body weight ratio,left ventricular weight/tibia length ratio were also increased significantly（P〈0.05）compared with corresponding control groups.ANP and BNP mRNA expression of TAC group were higher than those of swim group obviously（P〈0.01）,and its＇ myocardial fibrosis was significantly heavier than swim group.2）FFA in myocardium of TAC group were significantly increased compared with those of sham（P〈0.05）and swim group（P〈0.01）,Glu in myocardial of swim group were significantly higher than those of control（P〈0.05）and TAC group（P〈0.05）.（3）AMPK mRNA expression levels were significantly increased of swim group compared with93control（P〈0.05）and TAC group（P〈0.01）,while PPAR-αmRNA expression levels of TAC group were significantly lower than those of sham（P〈0.05）and swim group（P〈0.05）.Conclusion：Exercise could improve energy metabolism in cardiac hypertrophy,and reduce energy metabolism embryonic recapitulation through activating of AMPK expression.
