北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2014年
9期
61~67
,共null页
水通道蛋白2 慢性心力衰竭 间歇有氧运动 肾脏功能 大鼠
水通道蛋白2 慢性心力衰竭 間歇有氧運動 腎髒功能 大鼠
수통도단백2 만성심력쇠갈 간헐유양운동 신장공능 대서
aquaporin 2; chronic heart failure; aerobic interval training; renal function; rats
目的:探讨运动干预对慢性心力衰竭(CHF)大鼠肾脏功能和肾脏AQP2表达的影响,揭示运动改善CHF肾脏功能的可能机制。方法:选取雄性SD大鼠36只,随机分为假手术组(Sham),慢性心力衰竭组(CHF),慢性心力衰竭+间歇有氧运动组(AIT-CHF),每组12只。Sham组大鼠常规饲养,CHF采用心脏左冠状动脉前降支(LADCA)结扎法,建立CHF模型。AIT-CHF组大鼠在术后4周开始运动。AIT-CHF组起始运动速度为10 m/min,运动时间为10 min,速度逐渐增至25 m/min,运动7 min后,间歇3 min,其速度为15 m/min,之后依次交替进行,运动总时间为60 min。以上训练方案每周5 d,训练至8周结束。训练结束后,采用血流动力学和紫外分光光度法测定各组大鼠心肾功能变化。利用天狼猩红染色和免疫组化方法分别检测肾脏间质胶原和AQP2表达定位。运用Western Blot和Real time-PCR法分别检测肾脏AQP2蛋白和mRNA表达变化。Western Blot法检测各组大鼠肾脏p-NF-κBp65蛋白含量。结果:与Sham组比较,CHF组大鼠LVEDP升高,LVSP和±dp/dt max显著降低;CVF升高,血清BUN和sCr表达增高,AVP活性显著增强;CHF后可见肾脏集合管主细胞膜和胞浆中AQP2阳性染色,并且AQP2蛋白和mRNA表达显著升高,同时肾脏中p-NF-κBp65蛋白表达增多。与CHF组比较,AIT可显著降低AIT-CHF组大鼠LVEDP,升高LVSP和±dp/dt max;降低CVF,减少血清BUN和sCr表达,降低AVP活性表达;显著减少肾脏AQP2蛋白和mRNA表达,同时降低肾脏p-NF-κBp65蛋白表达。结论:1)间歇有氧运动通过降低血液中BUN和sCr表达,减少肾脏间质胶原沉积,缓解CHF大鼠肾脏功能紊乱。2)间歇有氧运动通过降低AVP活性,减少AQP2基因和蛋白表达,减缓CHF大鼠肾脏水潴留。3)间歇有氧运动干预改善CHF大鼠肾脏功能的机制可能与NF-κB信号转导通路有关。
目的:探討運動榦預對慢性心力衰竭(CHF)大鼠腎髒功能和腎髒AQP2錶達的影響,揭示運動改善CHF腎髒功能的可能機製。方法:選取雄性SD大鼠36隻,隨機分為假手術組(Sham),慢性心力衰竭組(CHF),慢性心力衰竭+間歇有氧運動組(AIT-CHF),每組12隻。Sham組大鼠常規飼養,CHF採用心髒左冠狀動脈前降支(LADCA)結扎法,建立CHF模型。AIT-CHF組大鼠在術後4週開始運動。AIT-CHF組起始運動速度為10 m/min,運動時間為10 min,速度逐漸增至25 m/min,運動7 min後,間歇3 min,其速度為15 m/min,之後依次交替進行,運動總時間為60 min。以上訓練方案每週5 d,訓練至8週結束。訓練結束後,採用血流動力學和紫外分光光度法測定各組大鼠心腎功能變化。利用天狼猩紅染色和免疫組化方法分彆檢測腎髒間質膠原和AQP2錶達定位。運用Western Blot和Real time-PCR法分彆檢測腎髒AQP2蛋白和mRNA錶達變化。Western Blot法檢測各組大鼠腎髒p-NF-κBp65蛋白含量。結果:與Sham組比較,CHF組大鼠LVEDP升高,LVSP和±dp/dt max顯著降低;CVF升高,血清BUN和sCr錶達增高,AVP活性顯著增彊;CHF後可見腎髒集閤管主細胞膜和胞漿中AQP2暘性染色,併且AQP2蛋白和mRNA錶達顯著升高,同時腎髒中p-NF-κBp65蛋白錶達增多。與CHF組比較,AIT可顯著降低AIT-CHF組大鼠LVEDP,升高LVSP和±dp/dt max;降低CVF,減少血清BUN和sCr錶達,降低AVP活性錶達;顯著減少腎髒AQP2蛋白和mRNA錶達,同時降低腎髒p-NF-κBp65蛋白錶達。結論:1)間歇有氧運動通過降低血液中BUN和sCr錶達,減少腎髒間質膠原沉積,緩解CHF大鼠腎髒功能紊亂。2)間歇有氧運動通過降低AVP活性,減少AQP2基因和蛋白錶達,減緩CHF大鼠腎髒水潴留。3)間歇有氧運動榦預改善CHF大鼠腎髒功能的機製可能與NF-κB信號轉導通路有關。
목적:탐토운동간예대만성심력쇠갈(CHF)대서신장공능화신장AQP2표체적영향,게시운동개선CHF신장공능적가능궤제。방법:선취웅성SD대서36지,수궤분위가수술조(Sham),만성심력쇠갈조(CHF),만성심력쇠갈+간헐유양운동조(AIT-CHF),매조12지。Sham조대서상규사양,CHF채용심장좌관상동맥전강지(LADCA)결찰법,건립CHF모형。AIT-CHF조대서재술후4주개시운동。AIT-CHF조기시운동속도위10 m/min,운동시간위10 min,속도축점증지25 m/min,운동7 min후,간헐3 min,기속도위15 m/min,지후의차교체진행,운동총시간위60 min。이상훈련방안매주5 d,훈련지8주결속。훈련결속후,채용혈류동역학화자외분광광도법측정각조대서심신공능변화。이용천랑성홍염색화면역조화방법분별검측신장간질효원화AQP2표체정위。운용Western Blot화Real time-PCR법분별검측신장AQP2단백화mRNA표체변화。Western Blot법검측각조대서신장p-NF-κBp65단백함량。결과:여Sham조비교,CHF조대서LVEDP승고,LVSP화±dp/dt max현저강저;CVF승고,혈청BUN화sCr표체증고,AVP활성현저증강;CHF후가견신장집합관주세포막화포장중AQP2양성염색,병차AQP2단백화mRNA표체현저승고,동시신장중p-NF-κBp65단백표체증다。여CHF조비교,AIT가현저강저AIT-CHF조대서LVEDP,승고LVSP화±dp/dt max;강저CVF,감소혈청BUN화sCr표체,강저AVP활성표체;현저감소신장AQP2단백화mRNA표체,동시강저신장p-NF-κBp65단백표체。결론:1)간헐유양운동통과강저혈액중BUN화sCr표체,감소신장간질효원침적,완해CHF대서신장공능문란。2)간헐유양운동통과강저AVP활성,감소AQP2기인화단백표체,감완CHF대서신장수저류。3)간헐유양운동간예개선CHF대서신장공능적궤제가능여NF-κB신호전도통로유관。
Objective: This paper aims at exploring the effects of aerobic interval training( AIT) on renal function and renal aquaporin- 2( AQP2) expression in rats with chronic heart failure( CHF) and investigating the possible mechanism of AIT improving renal dysfunction in CHF rats. Methods: Thirty-six male Sprague Dawley rats were randomly divided into three groups: Sham,CHF,and CHF combined AIT( AIT-CHF) groups( n = 12).The CHF model was established by ligating the left anterior descending coronary artery( LADCA) of rats. Four weeks after surgery,rats in AIT-CHF group underwent 8 weeks treadmill exercise training. Initially,rats in AITCHF group were exposed to adaptive exercise training at 10 m /min for 10 min,and then the exercise speed gradually increased to 25 m /min,and the time was 7 min,the interval rest was 3 min and rest speed was 15 m /min,both of exercise and interval rest changed alternately,60 min in total. This exercise training was performed 5 days per week,8 weeks in total. Rats' cardiac and renal functions were tested by haemodynamic parameters and spec-trophotometry. Pathological change in renal tissues was evaluated after Sirius red staining,and the collagen volume fraction( CVF) was calculated. The localizations of AQP2 expression was determined by immunohistochemistry.The protein and mRNA expression of AQP2 were detected by Western blot and quantitative real time-PCR,respectively. The protein expression of p-NF-κBp65 was examined by Western blot. Results: Compared with Sham group,left ventricular end-diastolic pressure( LVEDP) in CHF group increased while LVSP and ± dp /dt max decreased. When CVF increased,blood urea nitrogen( BUN),creatinine( sCr) in serum,and activity of arginine vasopressin( AVP) increased. AQP2 in renal collecting duct chief cell membrane and endochylema was positively stained. The protein and mRNA expressions of AQP2 increased. p-NF-κBp65 expression increased. Compared with CHF group,rats' LVEDP in AIT-CHF group decreased while their LVSP and ± dp /dt max increased. When CVF decreased,serum BUN,sCr and AVP activity decreased. Also after exercise,protein and mRNA expressions of AQP2,and protein expression of p-NF-κBp65 in renal tissue decreased. Conclusions: 1) Aerobic interval training can significantly improve the renal dysfunction in CHF rats by decreasing the expression of serum BUN and sCr,as well as renal interstitial collagen deposition. 2) Aerobic interval training significantly ameliorates water retention by reducing AVP activity and decreasing the gene and protein expression of AQP2. 3) The underlying mechanism of exercise improving renal function may be related to NF-κB signaling pathway.