CAJ | 학술논문

目的：观察一次性离心运动及针刺对大鼠股四头肌MyoD与Myogenin的影响,探讨针刺在骨骼肌损伤修复中的作用。方法：96只雄性SD大鼠随机编为安静（C）、安静针刺（CA）、运动（E）与运动针刺4组（EA）,E与EA组完成一次性跑台运动（坡度为-16°）;采用实时荧光定量PCR与免疫印迹法检测MyoD和Myogenin mRNA与蛋白表达。结果：CA组MyoD mRNA表达120 h显著高于C组（P〈0.05）,蛋白表达24 h、48 h显著高于C组（P〈0.05）;CA组Myogenin mRNA表达48 h、120 h显著高于C组（P〈0.05）,蛋白表达48 h后均显著高于C组（P〈0.05）。EA组MyoD mRNA表达12 h至72 h显著高于E组（P〈0.05）,蛋白表达12 h、24 h显著高于E组（P〈0.05）;EA组Myogenin mRNA表达均显著高于C组（P〈0.05）,12 h、48 h显著高于E组（P〈0.05）;蛋白表达12 h、120 h显著高于E组（P〈0.05）。结论：针刺正常骨骼肌、一次性离心运动及运动后针刺均能上调MyoD、Myogenin mRNA与蛋白表达,且针刺能快速增高运动后骨骼肌MyoD和Myogenin的蛋白表达。
목적：관찰일차성리심운동급침자대대서고사두기MyoD여Myogenin적영향,탐토침자재골격기손상수복중적작용。방법：96지웅성SD대서수궤편위안정（C）、안정침자（CA）、운동（E）여운동침자4조（EA）,E여EA조완성일차성포태운동（파도위-16°）;채용실시형광정량PCR여면역인적법검측MyoD화Myogenin mRNA여단백표체。결과：CA조MyoD mRNA표체120 h현저고우C조（P〈0.05）,단백표체24 h、48 h현저고우C조（P〈0.05）;CA조Myogenin mRNA표체48 h、120 h현저고우C조（P〈0.05）,단백표체48 h후균현저고우C조（P〈0.05）。EA조MyoD mRNA표체12 h지72 h현저고우E조（P〈0.05）,단백표체12 h、24 h현저고우E조（P〈0.05）;EA조Myogenin mRNA표체균현저고우C조（P〈0.05）,12 h、48 h현저고우E조（P〈0.05）;단백표체12 h、120 h현저고우E조（P〈0.05）。결론：침자정상골격기、일차성리심운동급운동후침자균능상조MyoD、Myogenin mRNA여단백표체,차침자능쾌속증고운동후골격기MyoD화Myogenin적단백표체。
Purpose： This study examined the effect of acute eccentric exercise and acupuncture on MyoD and Myogenin of quadriceps muscle in rats,and investigated the effect of acupuncture on injury repair in skeletal muscle.Methods： Ninety-six male SD rats were divided into four groups： control（ C）,control plus acupuncture（ CA）,exercise（E）,exercise plus acupuncture（EA） groups. E and EA groups underwent a single bout of eccentric exercise on a treadmill with- 16 o. mRNA and protein expression of MyoD and Myogenin were detected by real-time quantitative PCR and Western blot. Results： The mRNA expression of MyoD in CA group was significantly higher than that in C group at 120 h（ P〈0. 05）,and its protein expression in CA group was significantly higher than that in C goup at 24 h and 48 h（P〈0. 05）. Compared with C group,mRNA expression of Myogenin in CA group increased significantly at 48 h and 120 h（P〈0. 05）,and protein expresson in CA group increasd significantly at48 h（P〈0. 05）. Compared with E group,mRNA expression of MyoD in EA group was significantly higher from12 h to 72 h（ P〈0. 05）,and protein expresson in EA group was significantly higher at 12 h and 24 h（ P〈0. 05）. mRNA expression of Myogenin in EA group was higher than that in C group（P〈0. 05）,and higher than in E group at 12 h and 48 h（P〈0. 05）. Protein expression of Myogenin in EA group was higher than that in Egroup at 12 h and 120 h（P〈0. 05）. Conclusion： A single bout of eccentric exercise,acupuncture before and after exercise can up-regulate mRNA and protein expression of MyoD and Myogenin. Acupuncture after exercise can quickly raise MyoD and Myogenin protein expression in skeletal muscle.