武汉体育学院学报
武漢體育學院學報
무한체육학원학보
Journal of Wuhan Institute of Physical Education
2015年
2期
72~77
,共null页
王富鸿 李雪 邓文骞 张金梅 王璐 袁琼嘉
王富鴻 李雪 鄧文鶱 張金梅 王璐 袁瓊嘉
왕부홍 리설 산문건 장금매 왕로 원경가
过度负荷 学习记忆 GAP-43 海马 real—time PCR Western—blotting
過度負荷 學習記憶 GAP-43 海馬 real—time PCR Western—blotting
과도부하 학습기억 GAP-43 해마 real—time PCR Western—blotting
overload exercise;learning and memorizing;hippocampus;GAP-43; real time PCR;Western - blotting
目的：建立过度负荷运动模型，探讨过度负荷运动对大鼠空间学习记忆能力和海马的生长相关蛋白（GAP-43）的影响。方法：32只3月龄雄性SD大鼠，随机分4组（n=8）：空白组（CN）、水迷宫训练组（CM）、过度负荷运动组（ON）、过度负荷＋水迷宫训练组（OM）。ON、OM组进行过度负荷游泳训练，期间CN、CM组自然喂养；7周后CN、ON组即刻取材，CM、OM组进行10天的水迷宫训练，之后取材。采用HE染色法观察海马的形态结构，应用real—timePCR、Western—blotting定量分析海马的GAP-43表达情况。结果：①Morris水迷宫训练结果显示：OM组第2—7天较CM组同期的潜伏期非常显著增长（P〈0．01）；OM组第4-9天较第1—3天显著缩短（P〈0．05，P〈0．01），第6-9天较第4、5天显著缩短（P〈0．05，P〈0．01）；CM组的第4-9天各天较第1—3天均显著缩短（P〈O．05，P〈0．01）。OM细在空间探索实验中穿越平台的次数-9CM组相比没有显著差异（P〉O．05）。②HE染色观察结果：ON、OM组大鼠海马出现神经元排列较紊乱，组织结构疏松等现象。③GAP-43检测结果：ON组GAP-43表达非常显著上调于CN组（P〈0．01），OM组非常显著上调于CM、ON组（P〈0．01）。结论：空间-9习记忆能力的形成与GAP一43的基因上调有关，过度负荷运动造成海马神经元结构的轻微损伤，导致大鼠空间学习记忆能力的下降。过度负荷运动后GAP-43的基因表达上调主要参与修复损伤的海马神终元。
目的：建立過度負荷運動模型，探討過度負荷運動對大鼠空間學習記憶能力和海馬的生長相關蛋白（GAP-43）的影響。方法：32隻3月齡雄性SD大鼠，隨機分4組（n=8）：空白組（CN）、水迷宮訓練組（CM）、過度負荷運動組（ON）、過度負荷＋水迷宮訓練組（OM）。ON、OM組進行過度負荷遊泳訓練，期間CN、CM組自然餵養；7週後CN、ON組即刻取材，CM、OM組進行10天的水迷宮訓練，之後取材。採用HE染色法觀察海馬的形態結構，應用real—timePCR、Western—blotting定量分析海馬的GAP-43錶達情況。結果：①Morris水迷宮訓練結果顯示：OM組第2—7天較CM組同期的潛伏期非常顯著增長（P〈0．01）；OM組第4-9天較第1—3天顯著縮短（P〈0．05，P〈0．01），第6-9天較第4、5天顯著縮短（P〈0．05，P〈0．01）；CM組的第4-9天各天較第1—3天均顯著縮短（P〈O．05，P〈0．01）。OM細在空間探索實驗中穿越平檯的次數-9CM組相比沒有顯著差異（P〉O．05）。②HE染色觀察結果：ON、OM組大鼠海馬齣現神經元排列較紊亂，組織結構疏鬆等現象。③GAP-43檢測結果：ON組GAP-43錶達非常顯著上調于CN組（P〈0．01），OM組非常顯著上調于CM、ON組（P〈0．01）。結論：空間-9習記憶能力的形成與GAP一43的基因上調有關，過度負荷運動造成海馬神經元結構的輕微損傷，導緻大鼠空間學習記憶能力的下降。過度負荷運動後GAP-43的基因錶達上調主要參與脩複損傷的海馬神終元。
목적：건립과도부하운동모형，탐토과도부하운동대대서공간학습기억능력화해마적생장상관단백（GAP-43）적영향。방법：32지3월령웅성SD대서，수궤분4조（n=8）：공백조（CN）、수미궁훈련조（CM）、과도부하운동조（ON）、과도부하＋수미궁훈련조（OM）。ON、OM조진행과도부하유영훈련，기간CN、CM조자연위양；7주후CN、ON조즉각취재，CM、OM조진행10천적수미궁훈련，지후취재。채용HE염색법관찰해마적형태결구，응용real—timePCR、Western—blotting정량분석해마적GAP-43표체정황。결과：①Morris수미궁훈련결과현시：OM조제2—7천교CM조동기적잠복기비상현저증장（P〈0．01）；OM조제4-9천교제1—3천현저축단（P〈0．05，P〈0．01），제6-9천교제4、5천현저축단（P〈0．05，P〈0．01）；CM조적제4-9천각천교제1—3천균현저축단（P〈O．05，P〈0．01）。OM세재공간탐색실험중천월평태적차수-9CM조상비몰유현저차이（P〉O．05）。②HE염색관찰결과：ON、OM조대서해마출현신경원배렬교문란，조직결구소송등현상。③GAP-43검측결과：ON조GAP-43표체비상현저상조우CN조（P〈0．01），OM조비상현저상조우CM、ON조（P〈0．01）。결론：공간-9습기억능력적형성여GAP일43적기인상조유관，과도부하운동조성해마신경원결구적경미손상，도치대서공간학습기억능력적하강。과도부하운동후GAP-43적기인표체상조주요삼여수복손상적해마신종원。
The experiment built rates＇ excessive load to discuss the effect of excessive load on the memory abilities and the expres- sion of GAP-43 in hippocampus. 32 there month male SD rats were divided into 4 groups at random,8 rats every group in conTrol group （CN）, water maze training group （CM）, excessive load exercise group （ON）, water maze training -t- excessive load exer- cise group （OM）. The CN, CM group underwent natural feeding, ON and OM underwent excessive load swimming training. Af- ter 7 weeks CN and ON were collected materials immediately, CM and OM groups were collected materials after 10 days water maze behavior training. HE staining was used to observe the morphology of hippocampal structure, and the expression of GAP-43 gene in hippocampus was tested by Real-time PCR and Western blotting. Results： 1） The result of Morris water maze belmvior training, the inculmtion period of OM group was very significantly longer than CM group in 2-7 days（P〈0. 01） ; 4th -gth days of OM group was significantly shorter than its lst-3rd days（P〈0. 05,P〈0. 01）, its 6th -gth days were significantly shorter than4th -5th day（P〈0. 05, P〈0. 01 ）. The latent period of 4th - 9th days of CM group were significantly shorter than 1st -3rd days（P〈0. 05, P〈0. 01 ） ; the space exploration experiment showed that the time of going through platform of OM group has no significant difference with CM gmup（P〉0. 05）. 2. HE staining research results showed that the rats hippocanlpal neu-rons of ON and OM group were in disorder and the organization structure was loose etc. 3. The genetic tests of GAP-43 showed that ON group was very significantly higher than CN group（P〈0. 0l ）, OM group was very significantly higher than CM,ON group（P〈0. 01 ）. The formation of spatial learning memory ability was related in the raised GAP - 43. The excess load movement caused the hippocampus structure damage and led to a decline in spatial learning and memory ability of rats. The raised GAP-43 gene was main＇ly involved in repairing injury of hippocampalneurons after excess load movement.
