衡水学院学报
衡水學院學報
형수학원학보
Journal of Hengshui University
2015年
1期
33~35
,共null页
PHA 发酵 优化 正交实验
PHA 髮酵 優化 正交實驗
PHA 발효 우화 정교실험
PHA; fermentation; optimization; orthogonal experiment
以筛选出的生物环保材料PHA高产菌株SJ-9为初始菌株,对其发酵培养条件进行优化,以丁酸钠、碳氮比例、p H、温度、转速、装液量、误差7种因素,利用L18（37）正交试验设计进行条件优化,经实验得出适宜的发酵条件组合为：丁酸钠为2 g/L,碳氮比例为40,p H为7.5,温度为26℃,转速为150 r/min,装液量200 m L/L.此条件下,PHA产量为3.31 g/L.相对其未优化条件产量提高46.5%.
以篩選齣的生物環保材料PHA高產菌株SJ-9為初始菌株,對其髮酵培養條件進行優化,以丁痠鈉、碳氮比例、p H、溫度、轉速、裝液量、誤差7種因素,利用L18（37）正交試驗設計進行條件優化,經實驗得齣適宜的髮酵條件組閤為：丁痠鈉為2 g/L,碳氮比例為40,p H為7.5,溫度為26℃,轉速為150 r/min,裝液量200 m L/L.此條件下,PHA產量為3.31 g/L.相對其未優化條件產量提高46.5%.
이사선출적생물배보재료PHA고산균주SJ-9위초시균주,대기발효배양조건진행우화,이정산납、탄담비례、p H、온도、전속、장액량、오차7충인소,이용L18（37）정교시험설계진행조건우화,경실험득출괄의적발효조건조합위：정산납위2 g/L,탄담비례위40,p H위7.5,온도위26℃,전속위150 r/min,장액량200 m L/L.차조건하,PHA산량위3.31 g/L.상대기미우화조건산량제고46.5%.
With the selected high-producing strain S J-9 of the biological environmental protection material PHA as the initial strain, the fermentation and culture conditions are optimized with L18（37） orthogonal experiment, in terms of sodium butyrate, PH, temperature, rotate speed, loaded liquid and error. The experiment shows that the appropriate fermentation condition is got when sodium butyrate is 2 g/L, the ratio of carbon and nitrogen is 40, pH is 7.5, temperature is 26 ℃, rotate speed is 150 r/min and the loaded liquid is 200 mL/L. Under this optimized condition, the PHA production is 3.31 g/L which is increased by 46.5 % when compared to former conditions.
