北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2015年
1期
68~73
,共null页
游泳 大鼠 攻击行为 海马 5-HT1A-R 5-HT1B-R
遊泳 大鼠 攻擊行為 海馬 5-HT1A-R 5-HT1B-R
유영 대서 공격행위 해마 5-HT1A-R 5-HT1B-R
swimming; rats; aggressive behavior; hippocampus; 5-HT1A-R; 5-HT1B-R
目的：探讨游泳运动对攻击行为大鼠海马神经元5-HT1A-R、5-HT1B-R蛋白水平的表达变化,为运动改善大鼠攻击行为发生机制的阐明提供实验依据。方法：成年健康雄性SD大鼠36只,适应1周后随机分为：安静对照组、攻击模型组、攻击模型游泳组、入侵组。通过旷场实验检测大鼠的行为活动,采用Western-blot法及免疫组织化学法检测大鼠海马神经元5-HT1A受体、5-HT1B受体蛋白表达变化。结果：攻击模型建立成功后大鼠的总路程、平均速度、中央活动时间均较高,经过8周游泳运动干预后攻击模型大鼠的总路程、平均速度、中央活动时间均下降P〈0.05;攻击模型组大鼠海马神经元5-HT1A受体、5-HT1B受体蛋白表达明显下降（P〈0.05）,经过8周游泳运动干预后大鼠海马5-HT1A受体、5-HT1B受体蛋白表达明显升高（P〈0.05）。结论：游泳运动对攻击模型大鼠攻击行为具有改善作用,其作用机制可能与海马神经元5-HT1A受体、5-HT1B受体表达增强,使5-HT能系统功能提高,从而增强了中枢神经抑制作用有关。
目的：探討遊泳運動對攻擊行為大鼠海馬神經元5-HT1A-R、5-HT1B-R蛋白水平的錶達變化,為運動改善大鼠攻擊行為髮生機製的闡明提供實驗依據。方法：成年健康雄性SD大鼠36隻,適應1週後隨機分為：安靜對照組、攻擊模型組、攻擊模型遊泳組、入侵組。通過曠場實驗檢測大鼠的行為活動,採用Western-blot法及免疫組織化學法檢測大鼠海馬神經元5-HT1A受體、5-HT1B受體蛋白錶達變化。結果：攻擊模型建立成功後大鼠的總路程、平均速度、中央活動時間均較高,經過8週遊泳運動榦預後攻擊模型大鼠的總路程、平均速度、中央活動時間均下降P〈0.05;攻擊模型組大鼠海馬神經元5-HT1A受體、5-HT1B受體蛋白錶達明顯下降（P〈0.05）,經過8週遊泳運動榦預後大鼠海馬5-HT1A受體、5-HT1B受體蛋白錶達明顯升高（P〈0.05）。結論：遊泳運動對攻擊模型大鼠攻擊行為具有改善作用,其作用機製可能與海馬神經元5-HT1A受體、5-HT1B受體錶達增彊,使5-HT能繫統功能提高,從而增彊瞭中樞神經抑製作用有關。
목적：탐토유영운동대공격행위대서해마신경원5-HT1A-R、5-HT1B-R단백수평적표체변화,위운동개선대서공격행위발생궤제적천명제공실험의거。방법：성년건강웅성SD대서36지,괄응1주후수궤분위：안정대조조、공격모형조、공격모형유영조、입침조。통과광장실험검측대서적행위활동,채용Western-blot법급면역조직화학법검측대서해마신경원5-HT1A수체、5-HT1B수체단백표체변화。결과：공격모형건립성공후대서적총로정、평균속도、중앙활동시간균교고,경과8주유영운동간예후공격모형대서적총로정、평균속도、중앙활동시간균하강P〈0.05;공격모형조대서해마신경원5-HT1A수체、5-HT1B수체단백표체명현하강（P〈0.05）,경과8주유영운동간예후대서해마5-HT1A수체、5-HT1B수체단백표체명현승고（P〈0.05）。결론：유영운동대공격모형대서공격행위구유개선작용,기작용궤제가능여해마신경원5-HT1A수체、5-HT1B수체표체증강,사5-HT능계통공능제고,종이증강료중추신경억제작용유관。
Objective：The purpose of this study was to test the effect of swimming on hippocampus neurons 5-HT1A-R and 5-HT1B-R protein levels in rats with aggressive behavior,for providing references to explain the mechanism of exercise alleviating aggressive behavior.Methods：Thirty-six SD male rats were divided into following four groups after one-week adapted training,groups are：control group（C）,aggressive behavior group（A）,aggressive behavior and swimming group（AS）,and invasion group（I）.Rats＇ activity was tested by field experiment,hippocampus neurons 5-HT1A-R and 5-HT1B-R protein levels were measured by Western blotting and Immunohistochemistry.Results：The total distance,mean speed and central activity time in A group were higher than those in C group,but they were decreased in AS group（P 0.05） compared to A group.5-HT1A-R and 5-HT1B-R protein levels were lower in A group than those in C group（P 0.05）,but they were increased in AS group compared to A group（P 0.05）.Conclusion：Swimming can alleviate rats＇ aggressive behavior that is related to the increase of 5-HT1A-R and 5-HT1B-R after swimming that improves 5-HT system and enhances the inhibition effect of central nervous.
