北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2015年
2期
72~78
,共null页
田旭 郑澜 夏晓璇 文登台 陈珏君 奉悦 王卉
田旭 鄭瀾 夏曉璇 文登檯 陳玨君 奉悅 王卉
전욱 정란 하효선 문등태 진각군 봉열 왕훼
果蝇 Ca^2＋调控基因 心脏功能 基因敲减 运动抗衰老
果蠅 Ca^2＋調控基因 心髒功能 基因敲減 運動抗衰老
과승 Ca^2＋조공기인 심장공능 기인고감 운동항쇠로
drosophila; Ca^2＋ regulation gene; cardiac function; gene knockout; exercise anti-aging
目的：研究果蝇心肌细胞Ca^2＋调控相关基因敲减对増龄果蝇心脏功能及寿命的影响,筛选与心脏功能密切相关的Ca^2＋调控基因作为候选基因,为研究増龄果蝇基于Ca^2＋调控基因功能改变的心脏运动适应机制提供依据。方法：Hand-GAL4品系雄蝇、Ca^2＋调控相关基因UAS-RNAi品系雄蝇与野生型W1118处女蝇杂交,获得基因型为Hand/＋和calcium regulation gene RNAi/＋的F1代果蝇作为对照组果蝇;HandGAL4品系雄蝇与Ca^2＋调控相关基因UAS-RNAi品系处女蝇杂交,获得F1代基因型为Hand〉calcium regulation gene RNAi的果蝇为心肌组织Ca^2＋调控基因RNAi组果蝇。采用M-mode方法对1周龄（约为人类青少年时期）和5周龄（约为人类中老年时期）成体果蝇心脏功能衰退相关FS、AI、FL等指标进行检测,并统计各组果蝇存活情况,制作存活曲线。结果：Ca MKII基因心肌敲减果蝇与对照组相比：存活率明显下降,1周龄时表现为FS下降显著,FL明显增高,5周龄时表现为DI,FS明显下降,SI,AI和FL显著上升;5周龄Ca-α1D基因心肌敲减果蝇对比同龄对照组,DI、SI明显降低,DD、SD明显增高;5周龄Anx B9基因敲减果蝇AI较同龄对照组显著上升。结论：Ca MKII基因在维持増龄果蝇心脏功能和寿命中发挥着重要作用,Ca MKII、Ca-α1D和Anx B9可作为候选基因研究増龄果蝇心脏功能运动适应的潜在机制。
目的：研究果蠅心肌細胞Ca^2＋調控相關基因敲減對増齡果蠅心髒功能及壽命的影響,篩選與心髒功能密切相關的Ca^2＋調控基因作為候選基因,為研究増齡果蠅基于Ca^2＋調控基因功能改變的心髒運動適應機製提供依據。方法：Hand-GAL4品繫雄蠅、Ca^2＋調控相關基因UAS-RNAi品繫雄蠅與野生型W1118處女蠅雜交,穫得基因型為Hand/＋和calcium regulation gene RNAi/＋的F1代果蠅作為對照組果蠅;HandGAL4品繫雄蠅與Ca^2＋調控相關基因UAS-RNAi品繫處女蠅雜交,穫得F1代基因型為Hand〉calcium regulation gene RNAi的果蠅為心肌組織Ca^2＋調控基因RNAi組果蠅。採用M-mode方法對1週齡（約為人類青少年時期）和5週齡（約為人類中老年時期）成體果蠅心髒功能衰退相關FS、AI、FL等指標進行檢測,併統計各組果蠅存活情況,製作存活麯線。結果：Ca MKII基因心肌敲減果蠅與對照組相比：存活率明顯下降,1週齡時錶現為FS下降顯著,FL明顯增高,5週齡時錶現為DI,FS明顯下降,SI,AI和FL顯著上升;5週齡Ca-α1D基因心肌敲減果蠅對比同齡對照組,DI、SI明顯降低,DD、SD明顯增高;5週齡Anx B9基因敲減果蠅AI較同齡對照組顯著上升。結論：Ca MKII基因在維持増齡果蠅心髒功能和壽命中髮揮著重要作用,Ca MKII、Ca-α1D和Anx B9可作為候選基因研究増齡果蠅心髒功能運動適應的潛在機製。
목적：연구과승심기세포Ca^2＋조공상관기인고감대증령과승심장공능급수명적영향,사선여심장공능밀절상관적Ca^2＋조공기인작위후선기인,위연구증령과승기우Ca^2＋조공기인공능개변적심장운동괄응궤제제공의거。방법：Hand-GAL4품계웅승、Ca^2＋조공상관기인UAS-RNAi품계웅승여야생형W1118처녀승잡교,획득기인형위Hand/＋화calcium regulation gene RNAi/＋적F1대과승작위대조조과승;HandGAL4품계웅승여Ca^2＋조공상관기인UAS-RNAi품계처녀승잡교,획득F1대기인형위Hand〉calcium regulation gene RNAi적과승위심기조직Ca^2＋조공기인RNAi조과승。채용M-mode방법대1주령（약위인류청소년시기）화5주령（약위인류중노년시기）성체과승심장공능쇠퇴상관FS、AI、FL등지표진행검측,병통계각조과승존활정황,제작존활곡선。결과：Ca MKII기인심기고감과승여대조조상비：존활솔명현하강,1주령시표현위FS하강현저,FL명현증고,5주령시표현위DI,FS명현하강,SI,AI화FL현저상승;5주령Ca-α1D기인심기고감과승대비동령대조조,DI、SI명현강저,DD、SD명현증고;5주령Anx B9기인고감과승AI교동령대조조현저상승。결론：Ca MKII기인재유지증령과승심장공능화수명중발휘착중요작용,Ca MKII、Ca-α1D화Anx B9가작위후선기인연구증령과승심장공능운동괄응적잠재궤제。
Objective： The purposes of this study were to explore the effects of cardiac-specific Ca^2 ＋regulation genes knockout on cardiac function and lifespan in aging drosophila,screen Ca^2 ＋regulation genes as candidate genes of exercise anti-cardiac aging,and provide references for the mechanism of cardiac function adapting to exercise based on Ca^2 ＋regulation genes in aging drosophila. Methods： Male Hand-Cal4 and UAS-RNAi drosophila crossed with wild-type strain w1118 females respectively,and their filial generations were Hand / ＋,calcium regulation gene RNAi / ＋,and were collected as control group. Hand-Cal4 males and UAS-RNAi females were crossed and their filial generations were Hand calcium regulation gene RNAi,which were collected as cardiac specific Ca^2 ＋regulation gene knockout group. Heart function indicators,FS,AI and FL,of young drosophila（ 1- week old） or middle-age and old drosophila（ 5- week old） were examined by M-mode methods,and the survival rate of drosophila was recorded. Results： Compared with control group,survival rate of Ca MKII gene knockout group decreased significantly,FS of 1- week old drosophila decreased while FL increased,DI and FS of 5- week old drosophila decreased while their SI,AI and FL increased. Compared with control group with the same age,DI and SI of 5- week old drosophila with Ca-al D knockout decreased significantly while DD and SD increased,AI of 5-week old drosophila with Anx B9 knockout increased. Conclusion： Ca MKII plays an important role in heart function of aging drosophila and lifespan. Ca MKII,Ca-α1D and Anx B9 can be candidate genes for the mechanism of cardiac function adapting to exercise in aging drosophila.
