目的:通过大鼠骨骼肌急性损伤模型,研究跑台运动训练与按摩联合作用对损伤组织炎症发展过程中NADH还原酶,及肌卫星细胞增殖激活因子nNOS、HGF mRNA表达的影响,探讨跑台运动训练与按摩联合作用对骨骼肌急性损伤修复的作用机制。方法:选取76只SD雄性大鼠,随机分为5组,正常对照组(A组,n=4)、自然恢复组(B组,n=24)、按摩组(C组,n=16)、跑台组(D组,n=16),混合组(E组,n=16)。A组不做任何处理,B、C、D、E组采用自制改良打击器制备大鼠右侧腓肠肌急性损伤模型。B组不给予任何干预,C组于造模后第3天介入按摩,D组于第3天介入跑台运动训练,E组于第3天介入按摩、跑台运动训练。B、C、D、E组分别于制模后7d、14d、21d、28d采取实验大鼠腓肠肌样本,以HE染色观察组织形态学变化,Western Blot检测肌组织NADH还原酶蛋白量,实时荧光PCR检测肌卫星细胞中nNOS、HGF mRNA表达量。结果:肌运动跑台训练与按摩联合干预后,C、D、E组与B组比较,NADH表达量、nNOS与HGF mRNA表达量均高于B组,且具有显著性差异(P〈0.01);E组与C、D组比较,NADH表达量、nNOS与HGF mRNA表达量均高于C、D组,且具有极显著差异(P〈0.01);C组与D组比较,NADH表达量、nNOS与HGF mRNA表达量低于D组,且具有显著差异(P〈0.05)。HE染色显示,B组与A组比较,B组肌节紊乱,肌纤维碎裂,炎症细胞浸润,A组肌纤维结构完整,肌细胞排列规则,无炎症细胞。C、D、E组与B组比较,损伤部位血供更丰富,成肌细胞增殖明显,肌丝更多,恢复速度更快。结论:跑台运动训练与按摩联合治疗能明显提高骨骼肌急性损伤的组织中NADH还原酶蛋白量,nNOS、HGF mRNA表达量,促进炎症的消退和肌卫星细胞的增殖,提高骨骼肌急性损伤修复的速度。
目的:通過大鼠骨骼肌急性損傷模型,研究跑檯運動訓練與按摩聯閤作用對損傷組織炎癥髮展過程中NADH還原酶,及肌衛星細胞增殖激活因子nNOS、HGF mRNA錶達的影響,探討跑檯運動訓練與按摩聯閤作用對骨骼肌急性損傷脩複的作用機製。方法:選取76隻SD雄性大鼠,隨機分為5組,正常對照組(A組,n=4)、自然恢複組(B組,n=24)、按摩組(C組,n=16)、跑檯組(D組,n=16),混閤組(E組,n=16)。A組不做任何處理,B、C、D、E組採用自製改良打擊器製備大鼠右側腓腸肌急性損傷模型。B組不給予任何榦預,C組于造模後第3天介入按摩,D組于第3天介入跑檯運動訓練,E組于第3天介入按摩、跑檯運動訓練。B、C、D、E組分彆于製模後7d、14d、21d、28d採取實驗大鼠腓腸肌樣本,以HE染色觀察組織形態學變化,Western Blot檢測肌組織NADH還原酶蛋白量,實時熒光PCR檢測肌衛星細胞中nNOS、HGF mRNA錶達量。結果:肌運動跑檯訓練與按摩聯閤榦預後,C、D、E組與B組比較,NADH錶達量、nNOS與HGF mRNA錶達量均高于B組,且具有顯著性差異(P〈0.01);E組與C、D組比較,NADH錶達量、nNOS與HGF mRNA錶達量均高于C、D組,且具有極顯著差異(P〈0.01);C組與D組比較,NADH錶達量、nNOS與HGF mRNA錶達量低于D組,且具有顯著差異(P〈0.05)。HE染色顯示,B組與A組比較,B組肌節紊亂,肌纖維碎裂,炎癥細胞浸潤,A組肌纖維結構完整,肌細胞排列規則,無炎癥細胞。C、D、E組與B組比較,損傷部位血供更豐富,成肌細胞增殖明顯,肌絲更多,恢複速度更快。結論:跑檯運動訓練與按摩聯閤治療能明顯提高骨骼肌急性損傷的組織中NADH還原酶蛋白量,nNOS、HGF mRNA錶達量,促進炎癥的消退和肌衛星細胞的增殖,提高骨骼肌急性損傷脩複的速度。
목적:통과대서골격기급성손상모형,연구포태운동훈련여안마연합작용대손상조직염증발전과정중NADH환원매,급기위성세포증식격활인자nNOS、HGF mRNA표체적영향,탐토포태운동훈련여안마연합작용대골격기급성손상수복적작용궤제。방법:선취76지SD웅성대서,수궤분위5조,정상대조조(A조,n=4)、자연회복조(B조,n=24)、안마조(C조,n=16)、포태조(D조,n=16),혼합조(E조,n=16)。A조불주임하처리,B、C、D、E조채용자제개량타격기제비대서우측비장기급성손상모형。B조불급여임하간예,C조우조모후제3천개입안마,D조우제3천개입포태운동훈련,E조우제3천개입안마、포태운동훈련。B、C、D、E조분별우제모후7d、14d、21d、28d채취실험대서비장기양본,이HE염색관찰조직형태학변화,Western Blot검측기조직NADH환원매단백량,실시형광PCR검측기위성세포중nNOS、HGF mRNA표체량。결과:기운동포태훈련여안마연합간예후,C、D、E조여B조비교,NADH표체량、nNOS여HGF mRNA표체량균고우B조,차구유현저성차이(P〈0.01);E조여C、D조비교,NADH표체량、nNOS여HGF mRNA표체량균고우C、D조,차구유겁현저차이(P〈0.01);C조여D조비교,NADH표체량、nNOS여HGF mRNA표체량저우D조,차구유현저차이(P〈0.05)。HE염색현시,B조여A조비교,B조기절문란,기섬유쇄렬,염증세포침윤,A조기섬유결구완정,기세포배렬규칙,무염증세포。C、D、E조여B조비교,손상부위혈공경봉부,성기세포증식명현,기사경다,회복속도경쾌。결론:포태운동훈련여안마연합치료능명현제고골격기급성손상적조직중NADH환원매단백량,nNOS、HGF mRNA표체량,촉진염증적소퇴화기위성세포적증식,제고골격기급성손상수복적속도。
Objective: To investigate the effect of the combination of treadmill exercise and mas- sage on the inflammation NADH of the damage tissue and the expression of nNOS. HGF mR- NA in the satellite cell proliferation on the rehabilitation process of rats acute skeletal muscle injury so that to explore the role of massage on the rehabilitation to acute skeletal muscle. Methods:76 adult male SD rats were randomly divided into 5 groups: normal control (group A, n = 4 ), self- healing group ( group B, n = 20 ), control group of massage ( group C, n = 16 ), control of treadmill exercise ( group D, n = 16 ), control of mixture ( group E, n = 16 ). In group A, nothing were done ; In group B, C, D and E, rats were made to the model of rats with acute muscle injury by the improved traumatizing unit. Group t3 were not given the treatment, mas- sage were given to the group C, E and treadmill exercise were given to the group D, E after three days of the modeling. At 7, 14, 21,28days after the modeling, animal gastrocnemius samples were token in group B, C, D and E respectively, HE staining was employed to inspect pathological changes, the inflammation NADH was detected by the Western Blot, Real-time PCR was applied to detect the expression of nNOS, HGF mRNA in muscle satellite cells. Re- suits:After the combination interventionof massage and treadmill exercise, the expression of NADH, nNOS, HGF mRNA was higher in group C, D, E than the group B, and it has highly significant differences (P〈0.01). Compare to the group C and D, the expression of NADH, nNOS, HGF mRNA was more in the group E, and it has highly significant differences (P〈0. 01). Compare to the group C, the expression of nNOS, HGF mRNA of in Group D was high er,and it has significant differences (P〈0. 05). HE staining shows that myofilaments were disorder and ruptured, inflammatory cells were infiltrated, groud A was not. Compare to the group B, group C, D, E has more blood supply, more myofilaments, more activated muscle sat- ellite cells, necrotic tissue was rehabilitated faster compare to group B. Conclusion:The combi- nation of treadmill exercise can effectively improve the expression level of the NADH, nNOS, HGF mRNA in the muscle satellite cells, promote the inflammation to fade, the proliferation of satellite cell, provide the speed of the rehabilitation and regeneration in the damaged skeletal muscle.