CAJ | 학술논문

用荧光光谱法研究了模拟生理条件下磺脲类降糖药物格列美脲、格列本脲与牛血清白蛋白（BSA）的相互作用.结果表明：格列美脲、格列本脲均对BSA的荧光有猝灭作用,其猝灭机理均为静态猝灭.根据不同温度（298/297,302,310K）下格列美脲、格列本脲与BSA作用的荧光强度,由修正的Stern-Volmer方程计算了二者与BSA反应的结合常数,其值分别为2.239×105,1.857×105,1.247×105 L·mol-1和1.549×105,0.825 2×105,0.386 5×105 L·mol-1.根据van’t Hoff方程计算了反应的热力学参数,并讨论了格列美脲、格列本脲与BSA的主要作用力类型,其结果表明二者与BSA间的作用主要为氢键和范德华力.
용형광광보법연구료모의생리조건하광뇨류강당약물격렬미뇨、격렬본뇨여우혈청백단백（BSA）적상호작용.결과표명：격렬미뇨、격렬본뇨균대BSA적형광유졸멸작용,기졸멸궤리균위정태졸멸.근거불동온도（298/297,302,310K）하격렬미뇨、격렬본뇨여BSA작용적형광강도,유수정적Stern-Volmer방정계산료이자여BSA반응적결합상수,기치분별위2.239×105,1.857×105,1.247×105 L·mol-1화1.549×105,0.825 2×105,0.386 5×105 L·mol-1.근거van’t Hoff방정계산료반응적열역학삼수,병토론료격렬미뇨、격렬본뇨여BSA적주요작용력류형,기결과표명이자여BSA간적작용주요위경건화범덕화력.
The interactions of glimepiride/glibenclamide and bovine serum albumin（BSA）in physiological conditions solution（Tris-HCl buffer,pH 7.40）have been studied by fluorescence spectrometry.The results showed that the fluorescence intensity of BSA at 343 nm was quenched intensively by glimepiride/glibenclamide.According to the quenching effects of glimepiride/glibenclamide on the fluorescence intensity of BSA under different temperatures,and the quenching mechanisms were confirmed to be a static one.The binding constants and thermodynamic parameters were calculated.In the meantime,the main acting force between glimepiride/glibenclamide and bovine serum albumin（BSA）was discussed.The results showed the interaction force between glimepiride/glibenclamide and BSA belongs to hydrogen bond and Van der Waals force.