周口师范学院学报
週口師範學院學報
주구사범학원학보
Journal of Zhoukou Normal University
2015年
2期
94~96
,共null页
甘薯 豫薯12 茎尖分生组织 快繁
甘藷 豫藷12 莖尖分生組織 快繁
감서 예서12 경첨분생조직 쾌번
sweet potato; yu shu 12; stem tip meristem; rapid reproduction
以甘薯品种豫薯12的茎尖分生组织为材料,研究了不同培养基对其组培快繁的影响.结果表明：豫薯12最适基本培养基为MS培养基;在诱导茎尖成苗单因子实验中,最适宜的6-BA浓度为0.5 mg/L,最适宜的NAA浓度为0.01mg/L;在双因子实验中,诱导成苗最适宜培养基是MS＋6-BA 0.3 mg/L＋NAA 0.01mg/L;在茎段增殖实验中,最适宜的增殖培养基是MS＋GA30.1mg/L＋NAA 0.5mg/L.
以甘藷品種豫藷12的莖尖分生組織為材料,研究瞭不同培養基對其組培快繁的影響.結果錶明：豫藷12最適基本培養基為MS培養基;在誘導莖尖成苗單因子實驗中,最適宜的6-BA濃度為0.5 mg/L,最適宜的NAA濃度為0.01mg/L;在雙因子實驗中,誘導成苗最適宜培養基是MS＋6-BA 0.3 mg/L＋NAA 0.01mg/L;在莖段增殖實驗中,最適宜的增殖培養基是MS＋GA30.1mg/L＋NAA 0.5mg/L.
이감서품충예서12적경첨분생조직위재료,연구료불동배양기대기조배쾌번적영향.결과표명：예서12최괄기본배양기위MS배양기;재유도경첨성묘단인자실험중,최괄의적6-BA농도위0.5 mg/L,최괄의적NAA농도위0.01mg/L;재쌍인자실험중,유도성묘최괄의배양기시MS＋6-BA 0.3 mg/L＋NAA 0.01mg/L;재경단증식실험중,최괄의적증식배양기시MS＋GA30.1mg/L＋NAA 0.5mg/L.
With the stem tip meristem of sweet potato＂yu shu 12＂as test material,it was examined the effect of different medium on the tissue culture and rapid reproduction.The results showed as follows：the most suitable medium for yu shu 12 stem tip meristem is MS medium;In the reproducing experiments with single factor,the most suitable concentration of 6-BA is 0.5mg/L,the most suitable concentration of NAA is 0.01mg/L;In the double factor experiment,plantlet most suitable medium is MS＋6-BA 0.3mg/L＋NAA 0.01mg/L;In the shoots proliferation experiment,the most suitable medium is MS＋GA30.1mg/L ＋NAA 0.5mg/L.
