广州体育学院学报
廣州體育學院學報
엄주체육학원학보
Journal of Guangzhou Physical Education Institute
2015年
3期
100~104
,共null页
骨骼肌减少症 抗阻训练 衰老 股四头肌 总蛋白质 腺苷酸活化蛋白激酶
骨骼肌減少癥 抗阻訓練 衰老 股四頭肌 總蛋白質 腺苷痠活化蛋白激酶
골격기감소증 항조훈련 쇠로 고사두기 총단백질 선감산활화단백격매
sarcopenia; resistance training; aging; quadriceps ; total protein content; AMPK
目的：探讨不同负重抗阻训练对老年大鼠股四头肌总蛋白质含量和AMPK含量的影响.方法：以40只健康18月龄SD大鼠为研究对象,随机分为安静组、0％、30％、50％、70％最大负重组进行跑台抗阻训练,坡度35°,跑速15m/min,隔天一次,共8周.记录大鼠体重、摄食量和饮水量变化,用考马斯亮蓝蛋白测定法和酶联免疫吸附法分别测定大鼠股四头肌总蛋白含量和AMPK含量.结果：（1）与安静组和0％最大负重训练组相比,70％最大负重组大鼠体重出现了显著性下降（P＜0.01）;（2）与安静组相比,30％和50％最大负重训练组的股四头肌总蛋白质含量升高具有差异性（P＜0.05）;与0％最大负重训练组相比,30％最大负重训练组的股四头肌总蛋白质含量升高具有差异性（P＜0.05）;（3）与安静组和0％最大负重训练组相比,30％和50％最大负重训练组的股四头肌AMPK含量升高具有差异性（P＜0.01）.结论：8周不同负重量抗阻训练可提高老年大鼠股四头肌总蛋白质和AMPK的含量,且30％和50％最大负重量抗阻训练的效果更为明显,而运动对骨骼肌蛋白质合成及降解的调控表现出一定的矛盾性,具体机制需进一步探讨.
目的：探討不同負重抗阻訓練對老年大鼠股四頭肌總蛋白質含量和AMPK含量的影響.方法：以40隻健康18月齡SD大鼠為研究對象,隨機分為安靜組、0％、30％、50％、70％最大負重組進行跑檯抗阻訓練,坡度35°,跑速15m/min,隔天一次,共8週.記錄大鼠體重、攝食量和飲水量變化,用攷馬斯亮藍蛋白測定法和酶聯免疫吸附法分彆測定大鼠股四頭肌總蛋白含量和AMPK含量.結果：（1）與安靜組和0％最大負重訓練組相比,70％最大負重組大鼠體重齣現瞭顯著性下降（P＜0.01）;（2）與安靜組相比,30％和50％最大負重訓練組的股四頭肌總蛋白質含量升高具有差異性（P＜0.05）;與0％最大負重訓練組相比,30％最大負重訓練組的股四頭肌總蛋白質含量升高具有差異性（P＜0.05）;（3）與安靜組和0％最大負重訓練組相比,30％和50％最大負重訓練組的股四頭肌AMPK含量升高具有差異性（P＜0.01）.結論：8週不同負重量抗阻訓練可提高老年大鼠股四頭肌總蛋白質和AMPK的含量,且30％和50％最大負重量抗阻訓練的效果更為明顯,而運動對骨骼肌蛋白質閤成及降解的調控錶現齣一定的矛盾性,具體機製需進一步探討.
목적：탐토불동부중항조훈련대노년대서고사두기총단백질함량화AMPK함량적영향.방법：이40지건강18월령SD대서위연구대상,수궤분위안정조、0％、30％、50％、70％최대부중조진행포태항조훈련,파도35°,포속15m/min,격천일차,공8주.기록대서체중、섭식량화음수량변화,용고마사량람단백측정법화매련면역흡부법분별측정대서고사두기총단백함량화AMPK함량.결과：（1）여안정조화0％최대부중훈련조상비,70％최대부중조대서체중출현료현저성하강（P＜0.01）;（2）여안정조상비,30％화50％최대부중훈련조적고사두기총단백질함량승고구유차이성（P＜0.05）;여0％최대부중훈련조상비,30％최대부중훈련조적고사두기총단백질함량승고구유차이성（P＜0.05）;（3）여안정조화0％최대부중훈련조상비,30％화50％최대부중훈련조적고사두기AMPK함량승고구유차이성（P＜0.01）.결론：8주불동부중량항조훈련가제고노년대서고사두기총단백질화AMPK적함량,차30％화50％최대부중량항조훈련적효과경위명현,이운동대골격기단백질합성급강해적조공표현출일정적모순성,구체궤제수진일보탐토.
Objective：To discuss different weight resistance training on the content of total protein and AMPK in the aged rats quadriceps. Methods：The male SD rats as the research object, after feed to 18 months, they were assigned as 0% ,30% ,50% , 70% maximum weight resistance training intervention. The quadriceps content of total protein and AMPK were tested after a period of quote of the day for 8 weeks treadmill training with the speed of 15 m/min ,slope as 35°. Results： （1） Compared with quiet group and 0% maximum weighe training group, the weight of 70% maximum weighe training group decreased extremely significantly （P 〈 0.01 ） ; （2）Compared with quiet group, the quadriceps content of total protein of 30% and 50% maximum weight training groups increased significantly （ P 〈 0.05 ） ; Compared with 0% maximum weight training group, the quadriceps content of total protein of 30% maximum weight training group increased significantly （ P 〈 0.05 ） ; （3） Compared with quiet group and 0% maximum weight training group, the quadriceps AMPK content of 30% and 50% maximum weight training groups increased extremely significantly （P 〈 0.01 ）. Conclusion：8 weeks different weight resistance training can improve the content of total protein and AMPK in the aged rats quadriceps, especially 30% and 50% maximum weight resistance training. The specific mechanisms that exercise show some contradiction on skeletal muscle protein synthesis and degradation need to be further discussed.
