体育科学
體育科學
체육과학
China Sport Science
2015年
6期
57~63
,共null页
鲁妮 陈渝 邱方 石丽君
魯妮 陳渝 邱方 石麗君
로니 진투 구방 석려군
运动 高血压 钙离子通道 肠系膜动脉 运动强度
運動 高血壓 鈣離子通道 腸繫膜動脈 運動彊度
운동 고혈압 개리자통도 장계막동맥 운동강도
exercise ; hypertension ; calcium channel ; mesenteric artery ; exercise intensity
目的:运动是一种简单、有效的高血压非药物辅助疗法,选择适宜的运动强度和运动量是十分重要的。位于VSMC细胞质膜上的L型钙通道(CaV1.2)对血管紧张度的调节具有关键作用,CaV1.2通道表达上调是高血压的标志特征。旨在探讨不同强度运动对自发性高血压大鼠(SHR)肠系膜动脉CaV1.2通道重构的影响。方法:54只SHR大鼠随机分为安静对照组(SHR-SED,n=18)、中等强度运动组(SHR-M,18~20 m/min,n=18)和大强度运动组(SHR-H,26~28m/min,n=18),运动组进行8周跑台运动(1h/d,5d/周)。另选用同龄Wistar-Kyoto大鼠(WKY)18只作为安静正常血压对照组。观察不同强度运动对肠系膜动脉舒缩特性、CaV1.2通道功能和蛋白表达的影响。结果:1)大鼠安静时收缩压和心率:SHR-SED组显著高于WKY组,SHR-M组较SHR-SED组显著降低,而SHR-H组明显升高;2)硝苯吡啶(CaV1.2通道阻断剂)诱发了大鼠肠系膜动脉剂量依赖性的血管舒张,各组大鼠对硝苯吡啶的敏感性依次为:SHR-H〉SHR-SED〉SHR-M〉WKY,中等强度运动使SHR肠系膜动脉对硝苯吡啶的敏感性明显减弱,相反,大强度运动使其明显增强;3)SHR-SED组肠系膜动脉平滑肌CaV1.2通道电流和CaV1.2通道α1c亚基的蛋白表达均高于WKY组,与SHR-SED组相比,SHR-M组CaV1.2通道电流和CaV1.2通道α1c亚基的蛋白表达降低,而SHR-H组显著增加。结论:高血压可引起肠系膜动脉平滑肌细胞CaV1.2通道功能和蛋白表达上调;中等强度的有氧运动能有效逆转高血压动脉CaV1.2通道的重构,对改善血管功能具有积极作用,而大强度运动通过加重CaV1.2通道的不良重构进一步损害血管功能。
目的:運動是一種簡單、有效的高血壓非藥物輔助療法,選擇適宜的運動彊度和運動量是十分重要的。位于VSMC細胞質膜上的L型鈣通道(CaV1.2)對血管緊張度的調節具有關鍵作用,CaV1.2通道錶達上調是高血壓的標誌特徵。旨在探討不同彊度運動對自髮性高血壓大鼠(SHR)腸繫膜動脈CaV1.2通道重構的影響。方法:54隻SHR大鼠隨機分為安靜對照組(SHR-SED,n=18)、中等彊度運動組(SHR-M,18~20 m/min,n=18)和大彊度運動組(SHR-H,26~28m/min,n=18),運動組進行8週跑檯運動(1h/d,5d/週)。另選用同齡Wistar-Kyoto大鼠(WKY)18隻作為安靜正常血壓對照組。觀察不同彊度運動對腸繫膜動脈舒縮特性、CaV1.2通道功能和蛋白錶達的影響。結果:1)大鼠安靜時收縮壓和心率:SHR-SED組顯著高于WKY組,SHR-M組較SHR-SED組顯著降低,而SHR-H組明顯升高;2)硝苯吡啶(CaV1.2通道阻斷劑)誘髮瞭大鼠腸繫膜動脈劑量依賴性的血管舒張,各組大鼠對硝苯吡啶的敏感性依次為:SHR-H〉SHR-SED〉SHR-M〉WKY,中等彊度運動使SHR腸繫膜動脈對硝苯吡啶的敏感性明顯減弱,相反,大彊度運動使其明顯增彊;3)SHR-SED組腸繫膜動脈平滑肌CaV1.2通道電流和CaV1.2通道α1c亞基的蛋白錶達均高于WKY組,與SHR-SED組相比,SHR-M組CaV1.2通道電流和CaV1.2通道α1c亞基的蛋白錶達降低,而SHR-H組顯著增加。結論:高血壓可引起腸繫膜動脈平滑肌細胞CaV1.2通道功能和蛋白錶達上調;中等彊度的有氧運動能有效逆轉高血壓動脈CaV1.2通道的重構,對改善血管功能具有積極作用,而大彊度運動通過加重CaV1.2通道的不良重構進一步損害血管功能。
목적:운동시일충간단、유효적고혈압비약물보조요법,선택괄의적운동강도화운동량시십분중요적。위우VSMC세포질막상적L형개통도(CaV1.2)대혈관긴장도적조절구유관건작용,CaV1.2통도표체상조시고혈압적표지특정。지재탐토불동강도운동대자발성고혈압대서(SHR)장계막동맥CaV1.2통도중구적영향。방법:54지SHR대서수궤분위안정대조조(SHR-SED,n=18)、중등강도운동조(SHR-M,18~20 m/min,n=18)화대강도운동조(SHR-H,26~28m/min,n=18),운동조진행8주포태운동(1h/d,5d/주)。령선용동령Wistar-Kyoto대서(WKY)18지작위안정정상혈압대조조。관찰불동강도운동대장계막동맥서축특성、CaV1.2통도공능화단백표체적영향。결과:1)대서안정시수축압화심솔:SHR-SED조현저고우WKY조,SHR-M조교SHR-SED조현저강저,이SHR-H조명현승고;2)초분필정(CaV1.2통도조단제)유발료대서장계막동맥제량의뢰성적혈관서장,각조대서대초분필정적민감성의차위:SHR-H〉SHR-SED〉SHR-M〉WKY,중등강도운동사SHR장계막동맥대초분필정적민감성명현감약,상반,대강도운동사기명현증강;3)SHR-SED조장계막동맥평활기CaV1.2통도전류화CaV1.2통도α1c아기적단백표체균고우WKY조,여SHR-SED조상비,SHR-M조CaV1.2통도전류화CaV1.2통도α1c아기적단백표체강저,이SHR-H조현저증가。결론:고혈압가인기장계막동맥평활기세포CaV1.2통도공능화단백표체상조;중등강도적유양운동능유효역전고혈압동맥CaV1.2통도적중구,대개선혈관공능구유적겁작용,이대강도운동통과가중CaV1.2통도적불량중구진일보손해혈관공능。
Almtract: Objective: Exercise can be considered as a simple and effective non-drug treatment of hypertension. Choosing appropriate intensity and volume is supposed to be of great importance. L-type Ca^2+ (Car 1.2) channels on the plasma membrane of vascular smooth muscle cells play a key role in modulating vascular tone. Cav 1.2 channels upregulation is an ionic feature of hypertension. This study aimed to explore the effects of different intensity of exercise on Cav 1.2 channel remodeling of mesenteric artery (MA) from SHR. Methods.. Twelve-week-old male SHR rats were randomly divided into SHR sedentary group (SHR-SED, n= 18), SHR Moderate-(SHR-M, n= 18,18-20 m/rain) and high-intensity ( SHR-H, n= 18,26 - 28 m/rain) of exercise training exercise group. Eighteen age-matched WKY rats were used as normotensive control. Mesenteric arterial mechanical and functional properties were evaluated. Results:Moderate-intensity of exercise training induced lower systolic blood pressure and heart rate than those of SHR-SED. Moderate-intensity of exercise training significantly suppressed tissue sensitivity to nifedipine, Cavl. 2 channel currents density, and Cavl. 2-α1C subunit protein expression in MAs. However, high-intensity of exercise aggravated all of these hypertension-associated functional and molecular alterations of Car 1. 2 channel. Conclusion: Remodeling of MAs contributes to the development and complications of hypertension. The moderate-intensity exercise can effectively reverse the remodeling of Car 1.2 channels in mesenteric artery, which has a positive effect on improving vascular function. High-intensity exercise would exaggerate the adverse remodeling of Cav 1.2 channel which impairs vascular function further.