山东体育科技
山東體育科技
산동체육과기
Shandong Sports Science & Technology
2015年
3期
87~89
,共null页
耐力运动 氧化应激 心肌组织 SD大鼠
耐力運動 氧化應激 心肌組織 SD大鼠
내력운동 양화응격 심기조직 SD대서
endurance exercise training; oxidative stress; myocardial tissue; SD rats
目的:观察不同强度耐力训练对雄性大鼠的心肌氧化应激水平影响,探讨增强心肌抗氧化能力的适宜训练方式。方法:选用6周龄健康雄性大鼠40只,建立大鼠耐力跑台模型。将选取的大鼠随机分为四组:安静对照组(C组,10只);低强度组(L组,10只);中等强度组(M组,10只);高强度组(H组,10只)。四组进行为期6周的跑台实验后,用生化方法分别检测各组大鼠的氧化应激相关指标包括:心肌组织的超氧化物歧化酶(SOD)活力、谷胱甘肽过氧化物酶(GSH-Px)活力以及丙二醛(MDA)的含量。结果:四组不同强度耐力运动训练6周后,结果发现,与安静对照组相比,心肌组织的SOD活力在中强度组显著上升,而在高强度组显著下降(P〈0.01);GSH-Px活力在低强度组和中等强度组显著上升,而在高强度组显著下降(P〈0.05或P〈0.01);与安静对照组相比,高强度组的心肌组织MDA水平显著增高(P〈0.01)。结论:低中强度的耐力训练能有效提高心肌的抗氧化能力,尤其是中等强度的耐力训练。
目的:觀察不同彊度耐力訓練對雄性大鼠的心肌氧化應激水平影響,探討增彊心肌抗氧化能力的適宜訓練方式。方法:選用6週齡健康雄性大鼠40隻,建立大鼠耐力跑檯模型。將選取的大鼠隨機分為四組:安靜對照組(C組,10隻);低彊度組(L組,10隻);中等彊度組(M組,10隻);高彊度組(H組,10隻)。四組進行為期6週的跑檯實驗後,用生化方法分彆檢測各組大鼠的氧化應激相關指標包括:心肌組織的超氧化物歧化酶(SOD)活力、穀胱甘肽過氧化物酶(GSH-Px)活力以及丙二醛(MDA)的含量。結果:四組不同彊度耐力運動訓練6週後,結果髮現,與安靜對照組相比,心肌組織的SOD活力在中彊度組顯著上升,而在高彊度組顯著下降(P〈0.01);GSH-Px活力在低彊度組和中等彊度組顯著上升,而在高彊度組顯著下降(P〈0.05或P〈0.01);與安靜對照組相比,高彊度組的心肌組織MDA水平顯著增高(P〈0.01)。結論:低中彊度的耐力訓練能有效提高心肌的抗氧化能力,尤其是中等彊度的耐力訓練。
목적:관찰불동강도내력훈련대웅성대서적심기양화응격수평영향,탐토증강심기항양화능력적괄의훈련방식。방법:선용6주령건강웅성대서40지,건립대서내력포태모형。장선취적대서수궤분위사조:안정대조조(C조,10지);저강도조(L조,10지);중등강도조(M조,10지);고강도조(H조,10지)。사조진행위기6주적포태실험후,용생화방법분별검측각조대서적양화응격상관지표포괄:심기조직적초양화물기화매(SOD)활력、곡광감태과양화물매(GSH-Px)활력이급병이철(MDA)적함량。결과:사조불동강도내력운동훈련6주후,결과발현,여안정대조조상비,심기조직적SOD활력재중강도조현저상승,이재고강도조현저하강(P〈0.01);GSH-Px활력재저강도조화중등강도조현저상승,이재고강도조현저하강(P〈0.05혹P〈0.01);여안정대조조상비,고강도조적심기조직MDA수평현저증고(P〈0.01)。결론:저중강도적내력훈련능유효제고심기적항양화능력,우기시중등강도적내력훈련。
Objective: To analyze the effects of different types of endurance training on oxidative stress of myocardial tissue in Sprague-Dawley rats,and thereby provide rational movement scheme to improve the myocardial antioxidant capacity. Methods: A total of 40 male SD rats( 6weeks) were enrolled in the study. The study consisted of control group( n = 10),low intensity exercise group( n = 10),moderate intensity exercise group( n = 10) and highly intensity exercise group( n = 10). The endurance exercise training groups exercised for 6weeks and the control group maintained their daily eating habits. Suzperoxide dismutase( SOD) activity,glutathione peroxidase( GSH-Px) activity and malondialdehyde( MDA)content were measured after the 6 weeks experiment. Results: After 6 weeks endurance exercise training intervention,compared with control group,the SOD activity were higher in moderate intensity exercise group( P〈 0. 01),and lighter in highly intensity exercise group( P〈 0. 01),the GSH-Px activity were higher in low and moderate intensity exercise group( P〈 0. 05 or P〈 0. 01),and lighter in highly intensity exercise group( P〈 0. 01). Moreover,the levels of MDA significantly enhanced in highly intensity exercise group( P〈 0. 01). Conclusion:The low and moderate intensity exercise groups were effective in improving the myocardial antioxidant capacity of rats,particularly in moderate intensity exercise group.