山东体育学院学报
山東體育學院學報
산동체육학원학보
Journal of Shandong Institute Of Physical Education And Sports
2015年
3期
62~66
,共null页
剧烈运动 ROS/NF-κB信号通路 炎症因子 维生素C
劇烈運動 ROS/NF-κB信號通路 炎癥因子 維生素C
극렬운동 ROS/NF-κB신호통로 염증인자 유생소C
strenuous exercise; ROS / NF-κB signaling pathway; inflammatory factor; Vitamin C
目的：探讨补充维生素C对剧烈运动后人体血清炎症因子水平的影响及ROS/NF-κB信号通路在其中的作用。方法：随机选取20名健康男性在校大学生,分为实验组和对照组。运动开始前分别给予两组大学生维生素C片3.0 g和安慰剂口服。两组大学生分别按照75%VO2max进行跑台运动,持续时间为1 h,并于运动干预开始前10 min、结束后0 h和2 h分别采集静脉血,分离血清和血细胞,并利用ELISA法检测外周血IL-1、IL-6、IL-8含量;由血细胞中分离白细胞后,分别测定白细胞中活性氧（ROS）水平和IKKα激酶活性。结果：剧烈运动后,两组大学生外周血白细胞均明显升高,组间无明显差异;对照组外周血IL-1、IL-6、IL-8含量均表现出逐渐升高的趋势,而实验组未表现出此趋势,其中IL-1水平运动前后无明显变化。此外,两组大学生外周血白细胞中IKKα活性均表现出先升后降的变化趋势,其中实验组升高趋势明显低于对照组。而白细胞中ROS水平却表现出逐渐升高的趋势,其中以对照组升高趋势更明显,组间差异有统计学意义。结论：剧烈运动可以引起白细胞中ROS/NF-κB信号通路活化和血清炎症因子水平升高,而运动前补充维生素C可以有效抑制白细胞中ROS/NF-κB信号通路活化介导的血清炎症因子水平升高。
目的：探討補充維生素C對劇烈運動後人體血清炎癥因子水平的影響及ROS/NF-κB信號通路在其中的作用。方法：隨機選取20名健康男性在校大學生,分為實驗組和對照組。運動開始前分彆給予兩組大學生維生素C片3.0 g和安慰劑口服。兩組大學生分彆按照75%VO2max進行跑檯運動,持續時間為1 h,併于運動榦預開始前10 min、結束後0 h和2 h分彆採集靜脈血,分離血清和血細胞,併利用ELISA法檢測外週血IL-1、IL-6、IL-8含量;由血細胞中分離白細胞後,分彆測定白細胞中活性氧（ROS）水平和IKKα激酶活性。結果：劇烈運動後,兩組大學生外週血白細胞均明顯升高,組間無明顯差異;對照組外週血IL-1、IL-6、IL-8含量均錶現齣逐漸升高的趨勢,而實驗組未錶現齣此趨勢,其中IL-1水平運動前後無明顯變化。此外,兩組大學生外週血白細胞中IKKα活性均錶現齣先升後降的變化趨勢,其中實驗組升高趨勢明顯低于對照組。而白細胞中ROS水平卻錶現齣逐漸升高的趨勢,其中以對照組升高趨勢更明顯,組間差異有統計學意義。結論：劇烈運動可以引起白細胞中ROS/NF-κB信號通路活化和血清炎癥因子水平升高,而運動前補充維生素C可以有效抑製白細胞中ROS/NF-κB信號通路活化介導的血清炎癥因子水平升高。
목적：탐토보충유생소C대극렬운동후인체혈청염증인자수평적영향급ROS/NF-κB신호통로재기중적작용。방법：수궤선취20명건강남성재교대학생,분위실험조화대조조。운동개시전분별급여량조대학생유생소C편3.0 g화안위제구복。량조대학생분별안조75%VO2max진행포태운동,지속시간위1 h,병우운동간예개시전10 min、결속후0 h화2 h분별채집정맥혈,분리혈청화혈세포,병이용ELISA법검측외주혈IL-1、IL-6、IL-8함량;유혈세포중분리백세포후,분별측정백세포중활성양（ROS）수평화IKKα격매활성。결과：극렬운동후,량조대학생외주혈백세포균명현승고,조간무명현차이;대조조외주혈IL-1、IL-6、IL-8함량균표현출축점승고적추세,이실험조미표현출차추세,기중IL-1수평운동전후무명현변화。차외,량조대학생외주혈백세포중IKKα활성균표현출선승후강적변화추세,기중실험조승고추세명현저우대조조。이백세포중ROS수평각표현출축점승고적추세,기중이대조조승고추세경명현,조간차이유통계학의의。결론：극렬운동가이인기백세포중ROS/NF-κB신호통로활화화혈청염증인자수평승고,이운동전보충유생소C가이유효억제백세포중ROS/NF-κB신호통로활화개도적혈청염증인자수평승고。
Objective： To illustrate the effect of Vitamin C on the serum level of inflammatory factors and the role of ROS / NF- κB signaling pathway. Methods： 10 healthy male college students were randomly selected and equally divided into experiment group and control group. Vitamin C（ 3. 0 g）and placebo were given prior to treadmill running exercise for 1 h,the strength of which was equal to75% VO2 max. Venous blood was collected at 10 min before the beginning,0 h and 2 h after the end of the exercise,respectively. The levels of IL- 1,IL- 6 and IL- 8 were detected using ELISA method. The level of reactive oxygen species（ ROS） and the activity of IKKα in white blood cells were also measured. Results： After the strenuous exercise,the leukocyte number in peripheral blood was significantly increased in both groups,however,there was no significant differences. The levels of IL- 1,IL- 6 and IL- 8 in peripheral blood were gradually up- regulated in control group,but not in the experiment group. In addition,the level of IL- 1 was not changed by the exercise. The activity of IKKα elevated soon after the exercise but dropped finally in both groups. The intracellular ROS levels were both gradually raised in the two groups. Similarly,the activity of IKKα and the intracellular ROS levels in the experiment group were both significantly lower compared to the control group. Conclusion： The strenuous exercise could activate ROS / NF- κB signaling pathway and enhance the expression of serum inflammatory factor. Oral intake of Vitamin C prior to the exercise might suppress the expression of serum inflammatory factor induced by ROS / NF- κB signaling pathway.
