CAJ | 학술논문

目的：观察中药苁蓉菟丝子煎剂对长期大强度运动大鼠卵巢组织形态和细胞超微结构的影响，探讨该方剂对运动性月经失调的预防效果及作用机制。方法：将36只3月龄健康雌性SD大鼠随机分为三组：安静对照组（Q 组）、运动对照组（S 组）和中药干预组（M 组），每组12只。S组和 M 组大鼠每日进行一次大强度游泳运动，持续5周，M 组同期每日灌喂中药苁蓉菟丝子煎剂。训练5周后取大鼠卵巢组织，利用 HE染色、透射电镜等技术观察卵巢组织形态和卵巢细胞超微结构。结果：与 Q组相比，S组大鼠卵巢的生长卵泡、成熟卵泡和新鲜黄体数量都显著性减少（ P ＜0.05／ P ＜0.01）而闭锁卵泡增多（P ＜0.05），其颗粒细胞内线粒体、滑面内质网以及高尔基体等细胞器明显减少并含有大量脂滴；M 组大鼠卵巢的生长卵泡、成熟卵泡和新鲜黄体数量则比 S组增加（P ＜0.05／ P ＜0.01）而闭锁卵泡减少（P ＜0.05），其颗粒细胞内线粒体、滑面内质网以及高尔基体等细胞器明显增加而脂滴减少。结论：中药苁蓉菟丝子煎剂可有效预防和改善长期大强度运动对卵巢卵泡发育、成熟以及排卵的抑制作用，改善卵巢颗粒细胞的超微结构，促进其内分泌活动。
목적：관찰중약종용토사자전제대장기대강도운동대서란소조직형태화세포초미결구적영향，탐토해방제대운동성월경실조적예방효과급작용궤제。방법：장36지3월령건강자성SD대서수궤분위삼조：안정대조조（Q 조）、운동대조조（S 조）화중약간예조（M 조），매조12지。S조화 M 조대서매일진행일차대강도유영운동，지속5주，M 조동기매일관위중약종용토사자전제。훈련5주후취대서란소조직，이용 HE염색、투사전경등기술관찰란소조직형태화란소세포초미결구。결과：여 Q조상비，S조대서란소적생장란포、성숙란포화신선황체수량도현저성감소（ P ＜0.05／ P ＜0.01）이폐쇄란포증다（P ＜0.05），기과립세포내선립체、활면내질망이급고이기체등세포기명현감소병함유대량지적；M 조대서란소적생장란포、성숙란포화신선황체수량칙비 S조증가（P ＜0.05／ P ＜0.01）이폐쇄란포감소（P ＜0.05），기과립세포내선립체、활면내질망이급고이기체등세포기명현증가이지적감소。결론：중약종용토사자전제가유효예방화개선장기대강도운동대란소란포발육、성숙이급배란적억제작용，개선란소과립세포적초미결구，촉진기내분비활동。
Objective：To observe the effect of cistanche and dodder decoction of traditional Chinese medicine on the tissue morphology and cell ultrastructure of ovaries of rats that are engaged in long - term high intensity exercise so as to explore the protective effect of the prescription on athletic menstrual disorder and mechanism of action. Methods：36（3 - month - old）female Sprague - Dawley （SD）rats are divided randomly into three groups：qui-et control group（Q）,sport model group（S）and medicine intervention group（M）with12 rats in each group. The rats in the group S and M perform long - term high intensity swimming and the rats in the group M fed with cistanche and dodder decoction daily. Ovarian samples of those rats are obtained after sports training for 5 weeks. Then the techniques of HE straining and transmission electron are used to observe the changes of tissue morphology and cell ultrastructure of ovaries. Results：Compared with group Q,less growing follicles,mature follicles and fresh luteum corpus （P 〈 0. 05;P 〈 0. 01）and more atresia follicle （P 〈 0. 05）can be observed in the group S,but more growing follicles,more mature follicles,more fresh luteum corpus and less atresia follicle are witnesses in the ovaries of group M than those of group S （P 〈 0. 05;P 〈 0. 01）. Under Transmission Elec-tron Microscope,abundant organelles are found such as mitochondria and endoplasmic reticulum and many lipid droplets in granulose cell in the group Q and M,but in group M,less organelle and more lipid droplets can be observed. Conclusion：The cistanche and dodder decoction can effectively prevent the inhibition of development and maturation of ovarian follicle due to long - term high intensity exercise,improve the ultrastructures of ovari-an cells and promote the secretion activity.