中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2015年
8期
570-574
,共5页
何如%李骁扬%孙锐%田野
何如%李驍颺%孫銳%田野
하여%리효양%손예%전야
X射线%神经元%神经突起%海马%离体
X射線%神經元%神經突起%海馬%離體
X사선%신경원%신경돌기%해마%리체
X-rays%Neuron%Neurite%Hippocampus%In vitro
目的 探讨X射线照射对离体培养原代海马神经元突起生长的作用.方法 采用离体的方式培养原代海马神经元,分别给予0、2、4、8、10和12 Gy剂量照射,在照射后第1天和第3天,采用MTT法检测电离辐射对原代海马神经元死亡的影响,免疫荧光染色检测电离辐射对原代海马神经元神经突起的影响.结果 在照射后第1天和第3天,随着剂量的增加,海马神经元细胞死亡增多,差异有统计学意义(F=123.068、43.370,P＜0.05),但4、8、10和12 Gy照射组间差异无统计学意义(P＞0.05).不同剂量照射后第1天,神经突起长度、总树突分支长度(TDBL)差异均有统计学意义(F=9.169、7.856,P＜0.05),照后第3天神经突起长度、TDBL和总树突分支点数(TDBTN)差异均有统计学意义(F=23.797、6.565、6.021,P＜0.05).结论 X射线照射对离体培养的原代海马神经元突起的生长具有抑制作用.
目的 探討X射線照射對離體培養原代海馬神經元突起生長的作用.方法 採用離體的方式培養原代海馬神經元,分彆給予0、2、4、8、10和12 Gy劑量照射,在照射後第1天和第3天,採用MTT法檢測電離輻射對原代海馬神經元死亡的影響,免疫熒光染色檢測電離輻射對原代海馬神經元神經突起的影響.結果 在照射後第1天和第3天,隨著劑量的增加,海馬神經元細胞死亡增多,差異有統計學意義(F=123.068、43.370,P＜0.05),但4、8、10和12 Gy照射組間差異無統計學意義(P＞0.05).不同劑量照射後第1天,神經突起長度、總樹突分支長度(TDBL)差異均有統計學意義(F=9.169、7.856,P＜0.05),照後第3天神經突起長度、TDBL和總樹突分支點數(TDBTN)差異均有統計學意義(F=23.797、6.565、6.021,P＜0.05).結論 X射線照射對離體培養的原代海馬神經元突起的生長具有抑製作用.
목적 탐토X사선조사대리체배양원대해마신경원돌기생장적작용.방법 채용리체적방식배양원대해마신경원,분별급여0、2、4、8、10화12 Gy제량조사,재조사후제1천화제3천,채용MTT법검측전리복사대원대해마신경원사망적영향,면역형광염색검측전리복사대원대해마신경원신경돌기적영향.결과 재조사후제1천화제3천,수착제량적증가,해마신경원세포사망증다,차이유통계학의의(F=123.068、43.370,P＜0.05),단4、8、10화12 Gy조사조간차이무통계학의의(P＞0.05).불동제량조사후제1천,신경돌기장도、총수돌분지장도(TDBL)차이균유통계학의의(F=9.169、7.856,P＜0.05),조후제3천신경돌기장도、TDBL화총수돌분지점수(TDBTN)차이균유통계학의의(F=23.797、6.565、6.021,P＜0.05).결론 X사선조사대리체배양적원대해마신경원돌기적생장구유억제작용.
Objective To investigate the effect of X-ray irradiation on neurites growth of primary hippocampal neurons in vitro.Methods Primary hippocampal neuron culture in vitro were irradiated with 0,2,4,8,10,12 Gy of X-rays.In the first day and the third day after irradiation,the cell death of primary hippocampal neurons was detected by MTT method,and the morphological changes of primary hippocampal neuronal neurites were detected with immunofluorescence staining method.Results In the first day and third day after irradiation,the cell death of hippocampal neurons increased significantly (F =123.068,43.370,P ＜0.05),but there were no significant difference among 4,8,10,and 12 Gy irradiation groups.Immunofluorescence staining showed that,in the first day after irradiation,the neurite length and total dendritic branch length (TDBL) were significantly changed (F =9.169,7.856,P ＜0.05),and in the third day after radiation,the neurite length,TDBL and total dendritic branch tip number (TDBTN) were also altered (F =23.797,6.565,6.021,P ＜ 0.05).Conclusion X-ray irradiation can inhibit the growth of neurites in the primary hippocampal neurons in vitro.
