CAJ | 학술논문

目的:探讨色素上皮衍生因子(PEDF)下调缺氧诱导因子-1α(HIF-1α)抑制前列腺癌细胞侵袭转移的机制. 方法:培养人前列腺癌上皮细胞株(PC3),实验组和对照组中分别加入PEDF和PBS 溶液,通过划痕实验和 transwell 实验验证两组细胞侵袭转移的能力;RT-QPCR 检测两组细胞中 HIF-1α 的表达水平. 结果: 划痕实验和transwell实验结果表明PEDF抑制前列腺癌侵袭转移能力,PEDF下调HIF-1α的表达. 结论:PEDF通过下调HIF-1α的表达抑制前列腺癌细胞的侵袭转移能力,揭示其可能为肿瘤干预治疗的新靶点, 为治疗前列腺癌等恶性肿瘤提供充分的科学依据和高效候选药物.
목적:탐토색소상피연생인자(PEDF)하조결양유도인자-1α(HIF-1α)억제전렬선암세포침습전이적궤제. 방법:배양인전렬선암상피세포주(PC3),실험조화대조조중분별가입PEDF화PBS 용액,통과화흔실험화 transwell 실험험증량조세포침습전이적능력;RT-QPCR 검측량조세포중 HIF-1α 적표체수평. 결과: 화흔실험화transwell실험결과표명PEDF억제전렬선암침습전이능력,PEDF하조HIF-1α적표체. 결론:PEDF통과하조HIF-1α적표체억제전렬선암세포적침습전이능력,게시기가능위종류간예치료적신파점, 위치료전렬선암등악성종류제공충분적과학의거화고효후선약물.
Objective To explore the mechanism that PDEF inhibition the invasion and metastasis of prostate cancer cells by down-reg uLated HIF-1α. Methods PEDF and PBS as the experimental group and control group were add to the c uLture medium of human prostate cancer cell line (PC3), the wound healing and transwell experiment were carried out to observed the ability of invasion and metastasis. And HIF-1α expression was detect by RT-QPCR. ResuLts The res uLts of wound healing and transwell showed that PEDF inhibit the ability of invasion and metastasis of prostate cancer. PEDF down-reg uLated the expression of HIF-1α. Conclusions PEDF inhibit the invasion and metastasis of prostate cancer by down-reg uLated the expression of HIF-1α.It will be the new target of tumor intervention and for the treatment of prostate cancer and other malignant tumors to provide adequate scientific basis and efficient drug candidates.