CAJ | 학술논문

目的观察足三里穴位注射黄芪注射液对D-半乳糖致衰老小鼠皮肤超氧化物歧化酶(SOD)、羟脯氨酸(HYP)、丙二醇(MDA)含量的影响.方法将小鼠随机分为4组,即正常组、模型组、黄芪注射组和生理盐水注射组,每组15只.除正常组以外,剩余45只小鼠皮下注射D-半乳糖制作衰老模型.造模21 d后,黄芪组小鼠两侧足三里穴分别注射黄芪注射液0.01mL/d,生理盐水组小鼠在相同穴位注射等量生理盐水.进行21 d后,对全部小鼠血清和皮肤组织SOD活性、HYP含量、MDA含量进行测定.结果模型组小鼠血清与皮肤组织中的各项指标与对照组比较有显著性差异(P<0.01).与模型组相比,黄芪组小鼠血清与皮肤组织SOD活性、HYP含量显著升高,MDA含量显著降低(P<0.01);生理盐水组小鼠血清与皮肤组织中的各项指标也呈现显著性差异(P<0.05),但效果相比黄芪组稍差.结论黄芪穴位注射对D-半乳糖引起的皮肤衰老具有一定的预防与延缓作用.
목적 관찰족삼리혈위주사황기주사액대D-반유당치쇠로소서피부초양화물기화매(SOD)、간포안산(HYP)、병이순(MDA)함량적영향.방법 장소서수궤분위4조,즉정상조、모형조、황기주사조화생리염수주사조,매조15지.제정상조이외,잉여45지소서피하주사D-반유당제작쇠로모형.조모21 d후,황기조소서량측족삼리혈분별주사황기주사액0.01mL/d,생리염수조소서재상동혈위주사등량생리염수.진행21 d후,대전부소서혈청화피부조직SOD활성、HYP함량、MDA함량진행측정.결과 모형조소서혈청여피부조직중적각항지표여대조조비교유현저성차이(P<0.01).여모형조상비,황기조소서혈청여피부조직SOD활성、HYP함량현저승고,MDA함량현저강저(P<0.01);생리염수조소서혈청여피부조직중적각항지표야정현현저성차이(P<0.05),단효과상비황기조초차.결론 황기혈위주사대D-반유당인기적피부쇠로구유일정적예방여연완작용.
Objective To investigate the efficacy of acupoint injection of Astragalus on contents of SOD,HYP and MDA in skin of aging mice in-duced by D-galactose. Methods The mice were randomly divided into normal group,model group,Astragalus group,and saline group(n=15). Except for the normal control group,the mice of other groups were treated with continuous subcutaneous injection of D-galactose to establish mice ag-ing model. The Astragalus group mice was injected with 0.01mL Astragalus in Sanzuli acupoint per day,The saline group mice received an injection of same amount of saline. SOD,HYP and MDA changes in the skin tissue and serum were evaluated after 21 days. Results All the index in the skin tissue and serum showed significant difference(P<0.01)between model group mice and normal control group. Compared with model group,SOD activity and MDA content in the skin tissue and serum of the Astragalus group mice were significantly raised,HYP content was significantly reduced (P<0.01). There also were significant differences of these index between the saline group mice and the model group(P<0.05),but the effect was not as good as the Astragalus group mice. Conclusion Acupoint Injection of Astragalus can prevent and protect against skin aging induced by D-galactose.