实用医学杂志
實用醫學雜誌
실용의학잡지
The Journal of Practical Medicine
2015年
16期
2598-2601
,共4页
大肠肿瘤%p21-activatedkinase1%5-氟尿嘧啶%化疗增敏
大腸腫瘤%p21-activatedkinase1%5-氟尿嘧啶%化療增敏
대장종류%p21-activatedkinase1%5-불뇨밀정%화료증민
Colon cancer%p21-activated kinase 1%5-fluorouracil%Chemotherapy sensitivity
目的:探讨大肠癌细胞中 p21-活化激酶 1 ( PAK1 )对 5-氟尿嘧啶( 5-FU )化疗敏感性的影响.方法:CCK8 法测定增殖;流式细胞仪测定凋亡率;Hoechst 染色法检测凋亡;Western Blot 检测Bcl-xl、Bcl-2、XIAP表达. 结果:敲除PAK1可抑制LoVo细胞的生长,而5-FU联合shRNA-PAK1 组(联合组)细胞增殖受到最显著抑制(P < 0.05). 各组凋亡率:联合组(53.36 ± 4.03)%、shRNA-PAK1 转染组(26.48 ± 2.78)%、5-FU 组(15.52 ± 1.30)%,联合组凋亡率显著增高(P < 0.05). 联合组凋亡核所占比率即凋亡率最高 (P <0.01).联合组Bcl-xl、Bcl-2、XIAP的表达较单独5-FU组及shRNA-PAK1组抑制更加明显.结论: RNA干扰抑制PAK1表达后LoVo细胞对5-FU的敏感性增加,更显著抑制增殖、诱导凋亡.
目的:探討大腸癌細胞中 p21-活化激酶 1 ( PAK1 )對 5-氟尿嘧啶( 5-FU )化療敏感性的影響.方法:CCK8 法測定增殖;流式細胞儀測定凋亡率;Hoechst 染色法檢測凋亡;Western Blot 檢測Bcl-xl、Bcl-2、XIAP錶達. 結果:敲除PAK1可抑製LoVo細胞的生長,而5-FU聯閤shRNA-PAK1 組(聯閤組)細胞增殖受到最顯著抑製(P < 0.05). 各組凋亡率:聯閤組(53.36 ± 4.03)%、shRNA-PAK1 轉染組(26.48 ± 2.78)%、5-FU 組(15.52 ± 1.30)%,聯閤組凋亡率顯著增高(P < 0.05). 聯閤組凋亡覈所佔比率即凋亡率最高 (P <0.01).聯閤組Bcl-xl、Bcl-2、XIAP的錶達較單獨5-FU組及shRNA-PAK1組抑製更加明顯.結論: RNA榦擾抑製PAK1錶達後LoVo細胞對5-FU的敏感性增加,更顯著抑製增殖、誘導凋亡.
목적:탐토대장암세포중 p21-활화격매 1 ( PAK1 )대 5-불뇨밀정( 5-FU )화료민감성적영향.방법:CCK8 법측정증식;류식세포의측정조망솔;Hoechst 염색법검측조망;Western Blot 검측Bcl-xl、Bcl-2、XIAP표체. 결과:고제PAK1가억제LoVo세포적생장,이5-FU연합shRNA-PAK1 조(연합조)세포증식수도최현저억제(P < 0.05). 각조조망솔:연합조(53.36 ± 4.03)%、shRNA-PAK1 전염조(26.48 ± 2.78)%、5-FU 조(15.52 ± 1.30)%,연합조조망솔현저증고(P < 0.05). 연합조조망핵소점비솔즉조망솔최고 (P <0.01).연합조Bcl-xl、Bcl-2、XIAP적표체교단독5-FU조급shRNA-PAK1조억제경가명현.결론: RNA간우억제PAK1표체후LoVo세포대5-FU적민감성증가,경현저억제증식、유도조망.
Objective To investigate the effect of p21-activated kinase 1 on chemotherapy sensitivity of 5-fluorouracil. Methods Cell proliferation was measured by CCK8 and apoptosis rate by flow cytometry or Hoechst staining; the expression of Bcl-xl, Bcl-2, XIAP were determined by Western Blot. Results 5-FU combined shRNA-Pak1 group (combination group) could be significantly inhibited in terms of proliferation (P <0.05). The percentage of apoptosis rate in combined group was the highest and the difference among groups indicated statistical significance (P < 0.05). The expression of Bcl-xl, Bcl-2, XIAP in combination group was significantly inhibited compared with 5-FU group or shRNA-Pak1 group. Conclusion PAK1 inhibited by RNA interference can enhance chemotherapy sensitivity of 5-Fu on growth inhibition and apoptosis induction in colon cancer significantly.
