浙江创伤外科
浙江創傷外科
절강창상외과
Zhejiang Journal of Traumatic Surgery
2015年
4期
623-625
,共3页
大肠癌%CD4+IL-9+T细胞%IL-9%IL-10
大腸癌%CD4+IL-9+T細胞%IL-9%IL-10
대장암%CD4+IL-9+T세포%IL-9%IL-10
Rectal cancer%CD4+IL-9+T cell%IL-9%IL-10
目的 研究CD4+IL-9+T细胞因子IL-9、IL-10在大肠癌肿瘤免疫微环境中的表达水平,探讨其与临床病理的相关性. 方法 采集2014年6月1日至2014年10月8日,本院初次行手术治疗的45例大肠癌患者的肿瘤组织、对应癌旁组织及外周血(术前和术后),同时取15例健康志愿者外周血液.运用液相蛋白芯片检测技术检测上述组织和外周血中CD4+IL-9+T细胞主要细胞因子IL-9、IL-10的浓度.结果 细胞因子IL-9在大肠癌患者微环境中的浓度为(0.82±0.51)pg/ml,高于癌旁(0.56±0.52)pg/ml,P=0.0318;同时,它也高于术前、术后及健康人外周血中IL-9的浓度,P<0.05;细胞因子IL-10在大肠癌患者微环境中的浓度高于癌旁,分别为(0.87±0.43),(0.52±0.49)pg/ml,P<0.05,也高于术前、术后及健康人外周血中IL-10的浓度,P<0.05. 结论 大肠癌肿瘤微环境中的CD4+IL-9+T细胞高表达细胞因子IL-9、IL-10,且细胞因子IL-9表达水平与肿瘤Duke' s分期存在相关性,分期越高IL-9的蛋白水平越高.由此可见干扰IL-9、IL-10的信号通路或活性或许能够成为治疗大肠肿瘤的新靶点.
目的 研究CD4+IL-9+T細胞因子IL-9、IL-10在大腸癌腫瘤免疫微環境中的錶達水平,探討其與臨床病理的相關性. 方法 採集2014年6月1日至2014年10月8日,本院初次行手術治療的45例大腸癌患者的腫瘤組織、對應癌徬組織及外週血(術前和術後),同時取15例健康誌願者外週血液.運用液相蛋白芯片檢測技術檢測上述組織和外週血中CD4+IL-9+T細胞主要細胞因子IL-9、IL-10的濃度.結果 細胞因子IL-9在大腸癌患者微環境中的濃度為(0.82±0.51)pg/ml,高于癌徬(0.56±0.52)pg/ml,P=0.0318;同時,它也高于術前、術後及健康人外週血中IL-9的濃度,P<0.05;細胞因子IL-10在大腸癌患者微環境中的濃度高于癌徬,分彆為(0.87±0.43),(0.52±0.49)pg/ml,P<0.05,也高于術前、術後及健康人外週血中IL-10的濃度,P<0.05. 結論 大腸癌腫瘤微環境中的CD4+IL-9+T細胞高錶達細胞因子IL-9、IL-10,且細胞因子IL-9錶達水平與腫瘤Duke' s分期存在相關性,分期越高IL-9的蛋白水平越高.由此可見榦擾IL-9、IL-10的信號通路或活性或許能夠成為治療大腸腫瘤的新靶點.
목적 연구CD4+IL-9+T세포인자IL-9、IL-10재대장암종류면역미배경중적표체수평,탐토기여림상병리적상관성. 방법 채집2014년6월1일지2014년10월8일,본원초차행수술치료적45례대장암환자적종류조직、대응암방조직급외주혈(술전화술후),동시취15례건강지원자외주혈액.운용액상단백심편검측기술검측상술조직화외주혈중CD4+IL-9+T세포주요세포인자IL-9、IL-10적농도.결과 세포인자IL-9재대장암환자미배경중적농도위(0.82±0.51)pg/ml,고우암방(0.56±0.52)pg/ml,P=0.0318;동시,타야고우술전、술후급건강인외주혈중IL-9적농도,P<0.05;세포인자IL-10재대장암환자미배경중적농도고우암방,분별위(0.87±0.43),(0.52±0.49)pg/ml,P<0.05,야고우술전、술후급건강인외주혈중IL-10적농도,P<0.05. 결론 대장암종류미배경중적CD4+IL-9+T세포고표체세포인자IL-9、IL-10,차세포인자IL-9표체수평여종류Duke' s분기존재상관성,분기월고IL-9적단백수평월고.유차가견간우IL-9、IL-10적신호통로혹활성혹허능구성위치료대장종류적신파점.
Objective To investigate the expression of CD4+IL-9+T cytokines IL-9 and IL-10 in colorectal tumor immune micro-environment, and the relationship with clinical pathology. Methods Collected 45 cases of colorectal cancer tumor tissues and corresponding para cancer tissues and peripheral blood(before and after surgery, initial surgery treatement in our hospital between June 1 and October 8, 2014),and 15 healthy volunteers in pe-ripheral blood. The concentration of main cytokines IL-9 and IL-10 of CD4+IL-9+T cells in the tissue and peripheral blood were detected using liquid pro-tein chip detection technology. Results The concentration of IL-9 in the tumor micro-environment (0.82±0.51)pg/ml was higher than that in the adja-cent tissue (0.56±0.52)pg/ml, P=0.0318, it was also higher than that in the before or after operation,and healthy human peripheral blood, P<0.05. IL-10 in the tumor micro-environment was higher than that in the adjacent tissue, P<0.05,was also higher than that in the before or after operation and healthy human peripheral blood,P<0.05. Conclusion The cytokines IL-9, IL-10 of CD4+IL-9+T cells was highly expressed in the tumor micro-environment, correla-tion test showed that protein level of IL-9 was correlated with Duke's stage, higher stages with higher IL-9 level. Interference signal pathway or activity of IL-9, IL-10 may become a new target in the treatment of rectal cancer.
