CAJ | 학술논문

对短尾三齿线虫(Triodontophorus brevicauda)、锯齿三齿线虫(T.serratus)、小三齿线虫(T. minor)和日本三齿线虫 (T. nipponicus)核糖体内转录间隔区(ITS)序列进行扩增、测序和比较分析. 基于ITS序列,采用最大似然法(ML)构建系统发生树,分析4种三齿线虫与其他圆线虫的亲缘关系. 结果4种三齿线虫ITS1、5.8S和ITS2序列大小分别为370~376 bp、153 bp和322~334 bp;4种虫体ITS1核苷酸同源性为88.4%~100%,5.8S为100%,ITS2为82.9%~97.8%;进化树中盅口科线虫和圆形科线虫各形成一个大的分支,但盅口科中微小杯环线虫聚集在圆形科这一分支中;小三齿线虫与日本三齿线虫,短尾三齿线虫与锯齿三齿线虫各形成一小的分支,两两亲缘关系较近,4种三齿线虫与盆口属和食道齿属的关系较圆线属近研究结果可为三齿线虫的遗传进化和分子鉴定提供参考.
대단미삼치선충(Triodontophorus brevicauda)、거치삼치선충(T.serratus)、소삼치선충(T. minor)화일본삼치선충 (T. nipponicus)핵당체내전록간격구(ITS)서렬진행확증、측서화비교분석. 기우ITS서렬,채용최대사연법(ML)구건계통발생수,분석4충삼치선충여기타원선충적친연관계. 결과4충삼치선충ITS1、5.8S화ITS2서렬대소분별위370~376 bp、153 bp화322~334 bp;4충충체ITS1핵감산동원성위88.4%~100%,5.8S위100%,ITS2위82.9%~97.8%;진화수중충구과선충화원형과선충각형성일개대적분지,단충구과중미소배배선충취집재원형과저일분지중;소삼치선충여일본삼치선충,단미삼치선충여거치삼치선충각형성일소적분지,량량친연관계교근,4충삼치선충여분구속화식도치속적관계교원선속근연구결과가위삼치선충적유전진화화분자감정제공삼고.
The sequences of internal transcribed spacer (ITS) of ribosomal DNA of Triodontophorus brevicauda,T. serratus,T. minor and T. nipponicus were amplified,sequenced and analyzed. The phylogenetic relationships among four species of genus Triodontophorus and other Strongylids nematodes in the GenBank were re-constructed by using maximum likelihood(ML) based on the sequences of ITS. The results showed that the length of ITS1,5.8S and ITS2 of four nematodes of Triodontophorus sp. was 370-376 bp,153 bp,322-334 bp,while the nucleotide homology of ITS1,5.8S and ITS2 of these nematodes was 88.4%-100%,100%, 82.9%-97.8%,respectively. The phylogenetic tree was divided into two major branches,including Cyathostomidae and Strongylidae branches,but Cylicostephanus minutus in Cyathostomidae was located in the branch of Strongylidae nematode.T. minor and T. nipponicus were in one sister clade,and T. brevicauda and T. serratus were another one,which they had close relationships. The relationship of four nematodes with Craterostomum and Oesophagodontus was closer than that of Strongylus. These results laid a foundation for further study of the genetic evolution and molecular identification of the nematode of genus Triodontophorus.