CAJ | 학술논문

目的利用量子点(QDs)免疫荧光技术对卵巢癌SKOV3细胞上叶酸受体(FR)及人附睾蛋白(HE)4 进行定位,初步揭示 QDs多重染色在卵巢癌细胞体外成像的应用价值. 方法采用活泼酯法,构建不同发射波长的 QDs-链霉亲和素( SA)复合物,分别与生物素化-叶酸( FA)及生物素化-抗HE4抗体结合,形成特异性荧光探针,同时靶向结合SKOV3细胞,实现细胞双重染色. 结果构建的QD-FA及QD-抗 HE4 抗体靶向探针具有极强的特异性,量子点多重染色的荧光强度与单重染色相比差异无统计学意义(P>0.05). 量子点具有极强的光学稳定性. 结论量子点多重染色在卵巢癌细胞体外成像中具有一定的应用价值,可为卵巢癌早期检测提供一种新思路.
목적 이용양자점(QDs)면역형광기술대란소암SKOV3세포상협산수체(FR)급인부고단백(HE)4 진행정위,초보게시 QDs다중염색재란소암세포체외성상적응용개치. 방법 채용활발지법,구건불동발사파장적 QDs-련매친화소( SA)복합물,분별여생물소화-협산( FA)급생물소화-항HE4항체결합,형성특이성형광탐침,동시파향결합SKOV3세포,실현세포쌍중염색. 결과 구건적QD-FA급QD-항 HE4 항체파향탐침구유겁강적특이성,양자점다중염색적형광강도여단중염색상비차이무통계학의의(P>0.05). 양자점구유겁강적광학은정성. 결론 양자점다중염색재란소암세포체외성상중구유일정적응용개치,가위란소암조기검측제공일충신사로.
Objective To detect the folate receptor (FR) and human epididymis protein 4 (HE4) receptor localization in ovarian cancer SKOV3 cells simultaneously by quantum dots ( QDs) immunofluorescence technique, and validate the application value of multiplexed QDs staining.Methods The streptavidin ( SA)-QDs compounds with different emission wavelength was established by active ester method. These compounds could be coupled with biotinylated folate and biotinylated anti-HE4 antibody, forming specific fluorescent probes to target bind SKOV3 cells simultaneously, to realize cells double staining.Results (1) The QD-FA and QD-HE4 target probes had strong specifici-ty.(2) There was no significant difference in fluorescence intensity between QDs multiple staining and single staining.(3) QDs had strong optical stability.Conclusions Multiplexed QDs staining has its application value in ovarian cancer in vitro imaging and could be a new thinking in ovarian cancer early detection.