黑龙江八一农垦大学学报
黑龍江八一農墾大學學報
흑룡강팔일농은대학학보
Journal of Heilongjiang August First Land Reclamation University
2015年
4期
72-76
,共5页
王昊乾%孔令祖%申贵男%韩英浩
王昊乾%孔令祖%申貴男%韓英浩
왕호건%공령조%신귀남%한영호
萘醌类衍生物%活性氧%细胞衰老%鼠胚胎成纤维细胞
萘醌類衍生物%活性氧%細胞衰老%鼠胚胎成纖維細胞
내곤류연생물%활성양%세포쇠로%서배태성섬유세포
naphthoquinone derivative%reactive oxygen species%cellular senescence%mouse embryonic fibroblast
通过对萘醌类化合物的化学修饰,研究2-取代5,8-二甲氧基-1,4-萘醌类衍生物对鼠胚胎成纤维细胞(MEF)衰老的抑制作用. 应用噻唑蓝(MTT)、流式细胞术(FCM)、SA-β-gal染色等方法检测多种萘醌衍生物对细胞毒性、细胞内活性氧(ROS)水平和细胞衰老的作用,实验结果显示:2-苄硫基-5,8-二甲氧基-1,4-萘醌在降低其细胞毒性作用的同时,具有清除细胞内活性氧从而抑制细胞衰老的作用.
通過對萘醌類化閤物的化學脩飾,研究2-取代5,8-二甲氧基-1,4-萘醌類衍生物對鼠胚胎成纖維細胞(MEF)衰老的抑製作用. 應用噻唑藍(MTT)、流式細胞術(FCM)、SA-β-gal染色等方法檢測多種萘醌衍生物對細胞毒性、細胞內活性氧(ROS)水平和細胞衰老的作用,實驗結果顯示:2-芐硫基-5,8-二甲氧基-1,4-萘醌在降低其細胞毒性作用的同時,具有清除細胞內活性氧從而抑製細胞衰老的作用.
통과대내곤류화합물적화학수식,연구2-취대5,8-이갑양기-1,4-내곤류연생물대서배태성섬유세포(MEF)쇠로적억제작용. 응용새서람(MTT)、류식세포술(FCM)、SA-β-gal염색등방법검측다충내곤연생물대세포독성、세포내활성양(ROS)수평화세포쇠로적작용,실험결과현시:2-변류기-5,8-이갑양기-1,4-내곤재강저기세포독성작용적동시,구유청제세포내활성양종이억제세포쇠로적작용.
To study inhibitory effect of novel naphthoquinone derivative on cellular senescence,the chemical structure of naphthoquinone compounds was modified to study the inhibitory effect of 2-substitude 5,8-dimethoxy-1,4-naphthoquinone derivatives on mouse embryonic fibroblast(MEF) senescence. The cytotoxicity of a variety of naphthoquinone derivatives were tested by MTT assay,and were detected the intracellular ROS level and cellular senescence situation by flow cytometry (FCM) and SA-β-gal staining. All of results indicated that 2-benzylthio-5,8-dimethoxy-1,4naphthoquinone not only had the lower cytotoxicity,but also had the role to prevent cellular senescence by intracellular ROS.
