CAJ | 학술논문

以精品肝素为原料,研究磁化水结合过氧化氢制备低分子肝素(LMWH,2~8 kDa),并优化了LMWH制备的工艺参数. 通过对pH、降解温度、降解时间和H2O2浓度四个因素的单因素试验和响应面分析,结果表明:当pH7.1、温度60 ℃、降解时间4.5 h、H2O2浓度6.4%时,LMWH的平均分子量为4 050 Da,2~8 kDa的含量为88.57%, 抗-FXa效价为103.42 IU·mg-1,抗-FXa/抗-FIIa值为3.34,符合市场对LMWH的要求. 为磁化水结合H2O2制备LMWH提供了良好的试验基础.
이정품간소위원료,연구자화수결합과양화경제비저분자간소(LMWH,2~8 kDa),병우화료LMWH제비적공예삼수. 통과대pH、강해온도、강해시간화H2O2농도사개인소적단인소시험화향응면분석,결과표명:당pH7.1、온도60 ℃、강해시간4.5 h、H2O2농도6.4%시,LMWH적평균분자량위4 050 Da,2~8 kDa적함량위88.57%, 항-FXa효개위103.42 IU·mg-1,항-FXa/항-FIIa치위3.34,부합시장대LMWH적요구. 위자화수결합H2O2제비LMWH제공료량호적시험기출.
The fine heparin as raw material was used to prepare low molecular weight heparin (LMWH,2-8 kDa) by hydrogen peroxide with magnetized water, and the process parameters for preparation of LMWH was optimized. Four factors of pH, degradation temperature, degradation time, concentration of H2O2 were tested by single factor and response surface experiments analysis. The results showed that the optimum condition of LMWH preparation were pH 7.1, degradation temperature 60 ℃, degradation time 4.5 h, and concentration of H2O2 6.4%. Under such conditions, the average molecular weight of LMWH was 4 050 Da, content of 2-8 kDa was 88.57%, titer of anti-FXa was 103.42 IU·mg-1, anti-FXa/anti-FIIa was 3.34. These indicators of LMWH met the requirements of mart, which provide an useful experimental foundation for preparation of LMWH by hydrogen peroxide with magnetized water.