CAJ | 학술논문

目的:检测食管鳞癌患者CD44v5表达情况并分析其与临床病理特征之间的关系,探讨血清中CD44v5含量检测用于食管鳞癌筛检的应用价值. 方法: 采用RT-PCR技术和Western-Blot法分别检测CD44v5基因在食管鳞癌组织及癌旁组织中的表达,并采用酶联免疫吸附法(ELISA法)测定该蛋白在100例食管鳞癌患者及60例健康体检者血清中的含量. 结果:(1)CD44v5在食管鳞癌组织中的相对表达量高于癌旁食管组织,且CD44v5表达在不同TNM分期、癌组织浸润深度、有无淋巴结转移及分化程度中存在差异(P < 0.05). (2)CD44v5蛋白在食管鳞癌患者血清中的含量(31.308 ± 10.123) μg/L高于健康体检者(19.364 ± 1.680) μg/L,差异具有统计学意义(P < 0.01);(3) ELISA法检测血清中CD44v5含量诊断食管鳞癌试验中,ROC曲线下面积0.865,以健康体检者血清CD44v5含量的95%可信区间上限值作为阳性判断标准诊断效果:灵敏度91.0%,特异度60.0%. 结论:CD44v5的表达与食管鳞癌的发生发展及浸润转移有关;ELISA 法检测血清中CD44v5蛋白含量用于诊断食管鳞癌灵敏度高,或许可以用于食管癌的筛检.
목적:검측식관린암환자CD44v5표체정황병분석기여림상병리특정지간적관계,탐토혈청중CD44v5함량검측용우식관린암사검적응용개치. 방법: 채용RT-PCR기술화Western-Blot법분별검측CD44v5기인재식관린암조직급암방조직중적표체,병채용매련면역흡부법(ELISA법)측정해단백재100례식관린암환자급60례건강체검자혈청중적함량. 결과:(1)CD44v5재식관린암조직중적상대표체량고우암방식관조직,차CD44v5표체재불동TNM분기、암조직침윤심도、유무림파결전이급분화정도중존재차이(P < 0.05). (2)CD44v5단백재식관린암환자혈청중적함량(31.308 ± 10.123) μg/L고우건강체검자(19.364 ± 1.680) μg/L,차이구유통계학의의(P < 0.01);(3) ELISA법검측혈청중CD44v5함량진단식관린암시험중,ROC곡선하면적0.865,이건강체검자혈청CD44v5함량적95%가신구간상한치작위양성판단표준진단효과:령민도91.0%,특이도60.0%. 결론:CD44v5적표체여식관린암적발생발전급침윤전이유관;ELISA 법검측혈청중CD44v5단백함량용우진단식관린암령민도고,혹허가이용우식관암적사검.
Objective To detect the expression of CD44v5 in esophageal squamous carcinoma (ESCC) and analyze its relationship with clinical pathological features,so as to explore the value of detecting CD44v5 in serum for screening ESCC. Methods The CD44v5mRNA and protein in ESCC tissues and its corresponding adjacent non-carcinomatous tissues were detected by RT-PCR and Western-Blot. Enzyme-linked immunosorbent assay (ELISA) was applied to detect the protein in serum of 100 ESCC patients and 60 healthy subjects. Results (1)The expression of CD44v5 in esophageal squamous carcinoma tissues is higher than the tissue adjacent to carcinoma, and CD44v5 expression in different TNM stages, invasion depth, presence of lymph node metastasis and differentiation is with statistical differences(P < 0.05). (2)CD44v5 proteins in patients with ESCC (31.308 ± 10.123) μg/L is higher than healthy subjects (19.364 ± 1.680) μg/L, the difference is statistically significant (P < 0.01). (3)In the trial of using ELISA method to detect CD44v5 content in serum in the diagnosis of ESCC, the area under the ROC curve is 0.865. If the healthy subjects' serum content of the upper limit of 95%confidence interval was taken as a positive judgment standard, the diagnosis effect: sensitivity is 91.0%and speciality is 60.0%. Conclusion The expression of CD44v5 was related to the occurrence and lymph node metastasis of ESCC. Using ELISA method to detect the contents of CD44v5 proteins in serum, with a high sensitivity, can be used to screen ESCC.