CAJ | 학술논문

目的探讨唑来膦酸干预原发性骨质疏松( OP)模型鼠后骨髓脂肪含量变化规律. 方法 27只3月龄雌性SD大鼠随机均分为假手术组( SHAM)、去卵巢组( OVX)及OVX+唑来膦酸干预组( ZOL). 分别在术后0、6、12周行MRI水-脂分离成像及BMD扫描,获取骨髓脂肪分数( MFF)及BMD. 实验结束行骨髓脂肪细胞定量分析. 结果 OVX组MFF呈时序性增高而BMD呈时序性降低. 第6、12周时,OVX组MFF较基线时分别增高40.0%、69.4%( P<0.001 ). 第6周始组间MFF有显著性差异,而组间BMD至第12周时才有统计学差异(P<0.001). OVX组骨髓脂肪细胞密度、直径及面积百分比较SHAM组分别增高57.3%、29.5%、163%,较ZOL分别增高65%、22.2%、144%(P<0.001). ZOL组MFF及脂肪细胞定量参数与SHAM组无明显差异. 结论 ZOL保护骨受损同时,可能通过抑制骨髓脂肪含量而起作用.
목적 탐토서래련산간예원발성골질소송( OP)모형서후골수지방함량변화규률. 방법 27지3월령자성SD대서수궤균분위가수술조( SHAM)、거란소조( OVX)급OVX+서래련산간예조( ZOL). 분별재술후0、6、12주행MRI수-지분리성상급BMD소묘,획취골수지방분수( MFF)급BMD. 실험결속행골수지방세포정량분석. 결과 OVX조MFF정시서성증고이BMD정시서성강저. 제6、12주시,OVX조MFF교기선시분별증고40.0%、69.4%( P<0.001 ). 제6주시조간MFF유현저성차이,이조간BMD지제12주시재유통계학차이(P<0.001). OVX조골수지방세포밀도、직경급면적백분비교SHAM조분별증고57.3%、29.5%、163%,교ZOL분별증고65%、22.2%、144%(P<0.001). ZOL조MFF급지방세포정량삼수여SHAM조무명현차이. 결론 ZOL보호골수손동시,가능통과억제골수지방함량이기작용.
Objective To explore the effect of zoledronic acid ( ZA) in restoring marrow adipogenesis in estrogen-deficient rats. Methods Twenty-seven female SD rats were randomly divided equally into SHAM, ovariectomized ( OVX) , and OVX+ZOL groups.Marrow fat fraction (MFF) and bone mineral density (BMD) were measured at 0, 6, and 12 weeks.Marrow adipocytes were quantitatively evaluated by histopathology.Results OVX rats exhibited time-depend increase in MFF but decrease in BMD. The increase of MFF above baseline was 40.0% at week 6 and 69.4% at week 12, respectively (P<0.001).Compared with SHAM rats, MFF in OVX rats markedly elevated at week 6 but a reduction in BMD was first detected at week 12 ( P<0.001) .The adipocyte density, diameter, and volume in OVX rats increased by 57.3%, 29.5%, and 163%, respectively, compared with those in SHAM rats, and by 65%, 22.2%, and 144%, respectively, compared with those in ZOL rats (P<0.001).MFF and adipocyte parameters were not different between ZOL rats and SHAM rats.Conclusion A single dose of ZOL is able to reverse the marrow adiposity of OVX-induced osteoporosis.