CAJ | 학술논문

杜氏盐藻通过水热液化制备得到的生物油,先采用溶剂分割分别得到正己烷相、二氯甲烷相和乙醚相,再对二氯甲烷相进行柱层析分离纯化.正己烷相和乙醚相直接通过 GC-MS 和 FT-IR 进行分析,二氯甲烷相先经柱层析分离后结合 GC-MS、二次质谱和 FT-IR 等确认不同馏分的产物组成.研究结果表明:二氯甲烷相经柱层析分离可得到 16 个馏分,分别是石油醚馏分(A1),主要是烯烃类;石油醚:乙酸乙酯馏分(A2),主要是酸类化合物;石油醚:丙酮馏分(A3),主要是酰胺类;石油醚:甲醇馏分(A4),主要是烷烃类;甲醇馏分(A5),主要是十八碳烯酰胺.经柱层析分离纯化后,生物油的回收率高达 91.38%;获取较全的生物油组分信息,为藻类液化机理的分析和生物油的改质提供了依据.
두씨염조통과수열액화제비득도적생물유,선채용용제분할분별득도정기완상、이록갑완상화을미상,재대이록갑완상진행주층석분리순화.정기완상화을미상직접통과 GC-MS 화 FT-IR 진행분석,이록갑완상선경주층석분리후결합 GC-MS、이차질보화 FT-IR 등학인불동류분적산물조성.연구결과표명:이록갑완상경주층석분리가득도 16 개류분,분별시석유미류분(A1),주요시희경류;석유미:을산을지류분(A2),주요시산류화합물;석유미:병동류분(A3),주요시선알류;석유미:갑순류분(A4),주요시완경류;갑순류분(A5),주요시십팔탄희선알.경주층석분리순화후,생물유적회수솔고체 91.38%;획취교전적생물유조분신식,위조류액화궤리적분석화생물유적개질제공료의거.
In the present paper, the bio-oil produced from hydrothermal liquefaction of D. tertiolecta was divided into n-hexane, chloroform and diethyl ether fractions via solvent division at first, and then chloroform fraction was purified by column chromatography. The n-hexane and diethyl ether fractions were directly analyzed by GC-MS and FT-IR, while the separation products obtained from column chromatography of chloroform fraction were combined with secondary mass spectrometry and FT-IR to confirm the product information. The results showed that chloroform fraction can be divided into sixteen components including the petroleum ether fraction (A1) mainly in alkenes, petroleum ether:ethyl acetate fraction (A2) mainly in carboxylic acid, petroleum ether:acetone fraction (A3) mainly in amides, petroleum ether:methanol fraction (A4) mainly in higher alkanes, and methanol fraction (A5) mainly in amides. In addition, the product recovery was reached up to 91.38% after the purification via column chromatography. Meanwhile, some components, which cannot be directly detected by GC-MS, were identified after column chromatography. The results gave a comprehensive insight in the compositions of product obtained from microalgal liquefaction, which provided the basis on further understanding of HTL mechanism and bio-oil upgrading.