CAJ | 학술논문

目的:评价半导体激光对感染根管内粪肠球菌的杀菌效果. 方法:成功建立粪肠球菌感染人单根管恒牙模型50个,用ProTaper F3锉预备根管后随机分为5组(n=10),A组: 空白对照,不照射激光;另4组分别用不同功率、时间半导体激光照射根管内壁, B组:1. 0 W,10 s;C组:1. 0 W,20 s;D组:2. 0 W, 10 s;E组:2. 0 W,20 s. 各组标本均于处理前、后在根管内壁表面取样并进行细菌培养计数;然后再分别用F4、F5锉依次预备并收集两种深度的牙本质碎屑,经厌氧培养后采用麦氏比浊法进行菌落计数. 结果:①各组处理后的根管内壁细菌量均较处理前下降(P<0. 05),各激光组细菌清除率均高于对照组(P<0. 05),而各激光组之间两两相比差异无统计学意义(P>0. 05); ②各激光组均能使两种深度牙本质中的细菌量明显减少(P<0. 05);其中照射10 s时, 2. 0 W比1. 0 W的杀菌效果好(P<0. 05),而照射20 s时,不同功率的杀菌效果无显著差异(P>0. 05);用1. 0 W时,照射20 s比10 s的杀菌效果好(P<0. 05),而用2. 0 W时,照射10、20 s的杀菌效果无差异(P>0. 05). 结论:半导体激光对根管内壁及深层牙本质的粪肠球菌均有杀菌作用,其对牙本质深层的杀菌效果随着照射时间延长、功率增大而增强.
목적:평개반도체격광대감염근관내분장구균적살균효과. 방법:성공건립분장구균감염인단근관항아모형50개,용ProTaper F3촤예비근관후수궤분위5조(n=10),A조: 공백대조,불조사격광;령4조분별용불동공솔、시간반도체격광조사근관내벽, B조:1. 0 W,10 s;C조:1. 0 W,20 s;D조:2. 0 W, 10 s;E조:2. 0 W,20 s. 각조표본균우처리전、후재근관내벽표면취양병진행세균배양계수;연후재분별용F4、F5촤의차예비병수집량충심도적아본질쇄설,경염양배양후채용맥씨비탁법진행균락계수. 결과:①각조처리후적근관내벽세균량균교처리전하강(P<0. 05),각격광조세균청제솔균고우대조조(P<0. 05),이각격광조지간량량상비차이무통계학의의(P>0. 05); ②각격광조균능사량충심도아본질중적세균량명현감소(P<0. 05);기중조사10 s시, 2. 0 W비1. 0 W적살균효과호(P<0. 05),이조사20 s시,불동공솔적살균효과무현저차이(P>0. 05);용1. 0 W시,조사20 s비10 s적살균효과호(P<0. 05),이용2. 0 W시,조사10、20 s적살균효과무차이(P>0. 05). 결론:반도체격광대근관내벽급심층아본질적분장구균균유살균작용,기대아본질심층적살균효과수착조사시간연장、공솔증대이증강.
AIM:To evaluate the antibacterial efficacy of mechanical preparation and diode laser on entero-coccus faecalis in infected root canals in vitro. METHODS:50 single-rooted teeth of in vitro root canal enterococcus faecalis infection model were randomly divided into 5 groups (n=10). The root canals of the teeth in the control group were prepared by Protaper F3. After F3 preparation, the tooth canals in the other 4 groups were treated with diode laser for 10 s and 20 s with 1. 0 W or 2. 0 W, respectively. Microbiological samples were collected from the root canals be-fore and after irrigation. The dentin debris from two depths of the root canals were collected and cultured for turbidimet-ric count. A one-way ANOVA with the Tukey Test was conducted for the comparisons among the groups. RESULTS:The numbers of E. faecalis in all the groups decreased after treatment (P<0. 05). The bacterieidal ration (BR) of the treatment groups was higher than that of the control(P<0. 05). Between each 2 laser groups, P>0. 05. The number of E. faecalis from two different depths of dentin reduced in all the laser groups(P<0. 05). Under the condition of 10 s jrradiation, the disinfection was more effective with 2. 0 W than that with 1. 0 W, and there was no significant difference between 10 s irradiation and 20 s irradiation under the conditions of 2. 0 W irradiation. With 1. 0 W the dis-infection was more effective by 20 s irradiation than by 10 s, and with 2. 0 W there was no significant difference be-tween 10 s and 20 s irradiation. CONCLUSION:Diode laser is effective on root canal disinfection due to its effective penetration in dentins. The bactericidal effect enhances with increase of irradiation time and energy.