CAJ | 학술논문

坛紫菜是我国独有的栽培紫菜物种,而目前对坛紫菜分类学层面上仍缺乏有效的分子鉴定技术. 本研究通过PCR方法,对采自福建省莆田海区坛紫菜样品核糖体基因簇序列进行了测定,目的是为坛紫菜分类及种质鉴定提供分子生物学证据. 测定结果表明,坛紫菜样品的18S rDNA的全长序列长度为2953 bp;28S rDNA部分序列3603 bp,且不含内含子;5.8S rDNA长度为158 bp,该区域的5'端和3'端十分保守,在紫菜属中差异较小;ITS1的长度为334 bp;ITS2为678 bp. 已经测定IGS区的部分长度为3555 bp,该区域存在复杂二级结构. 通过对坛紫菜核糖体基因簇序列的分析表明,其能够成为紫菜分类鉴定与系统演化的分子标记.
단자채시아국독유적재배자채물충,이목전대단자채분류학층면상잉결핍유효적분자감정기술. 본연구통과PCR방법,대채자복건성보전해구단자채양품핵당체기인족서렬진행료측정,목적시위단자채분류급충질감정제공분자생물학증거. 측정결과표명,단자채양품적18S rDNA적전장서렬장도위2953 bp;28S rDNA부분서렬3603 bp,차불함내함자;5.8S rDNA장도위158 bp,해구역적5'단화3'단십분보수,재자채속중차이교소;ITS1적장도위334 bp;ITS2위678 bp. 이경측정IGS구적부분장도위3555 bp,해구역존재복잡이급결구. 통과대단자채핵당체기인족서렬적분석표명,기능구성위자채분류감정여계통연화적분자표기.
Pyropia haitanensis is a unique cultivated laver in China, while the lack of effective molecular identifi?cation technology affects the taxonomy of P. haitanensis. The study sequenced the nuclear ribosomal DNA (nrD?NA) cistron sequence of P. haitanensis from Putian, Fujiang Province by polymerase chain reaction (PCR), in or?der to provide molecular biological evidence for classification and germplasm identification of P. haitanensis. The results show that each unit is composed of the small-subunit (SSU) rRNA gene of which the full length is 2953bp, that the partial sequence of the large-subunit (LSU) rRNA gene of which the length is 3603bp without any introns, that the length of the 5.8S rRNA gene is 158bp, and the 5'and 3'terminal sequences of the genic regions of the ribosomal cistron are extremely conservative in the case of the genus of Pyropia, that the length of ITS1 is 334bp and ITS2 is 678bp and that the partial sequence of IGS is 3555bp, due to a complicated second?ary structure existing in IGS region, which makes sequencing untoward. Though the analysis of nuclear ribosom?al DNA sequence, it can be a molecular maker for the taxonomic identification of Pyropia.