CAJ | 학술논문

目的:研究肺炎链球菌脑膜炎中紧密连接的主要结构蛋白咬合蛋白(Occludin)在脑组织内表达的变化特点,探讨细菌性脑膜炎中血脑屏障的变化.方法:采用肺炎链球菌Ⅲ型菌株和等量的生理盐水脑池内注射,建立3周龄大鼠细菌性脑膜炎模型和生理盐水对照组,用免疫组化染色、Western blot技术,分别于感染后24 h、48 h、5 d后检测Occludin在脑组织内的表达. 结果:(1)Loeffler的神经行为学评分,24 h、48 h与5 d时造模组评分与生理盐水对照组比较差异有统计学意义(P < 0.05). (2)Occludin 的表达,肺炎链球菌感染后24 h 大鼠脑组织Occludin 的表达明显降低, 与生理盐水对照组相比, 差异有统计学意义 (P <0.05);48 h 后降至最低,与生理盐水对照组相比差异有统计学意义(P < 0.05);5 d 表达较 48 h 上升,但仍低于生理盐水对照组(P < 0.05). 结论:细菌感染中枢神经系统后,Occludin表达下降且有先下降后增高规律,提示Occludin参与了感染性脑水肿的发生、发展过程.
목적:연구폐염련구균뇌막염중긴밀련접적주요결구단백교합단백(Occludin)재뇌조직내표체적변화특점,탐토세균성뇌막염중혈뇌병장적변화.방법:채용폐염련구균Ⅲ형균주화등량적생리염수뇌지내주사,건립3주령대서세균성뇌막염모형화생리염수대조조,용면역조화염색、Western blot기술,분별우감염후24 h、48 h、5 d후검측Occludin재뇌조직내적표체. 결과:(1)Loeffler적신경행위학평분,24 h、48 h여5 d시조모조평분여생리염수대조조비교차이유통계학의의(P < 0.05). (2)Occludin 적표체,폐염련구균감염후24 h 대서뇌조직Occludin 적표체명현강저, 여생리염수대조조상비, 차이유통계학의의 (P <0.05);48 h 후강지최저,여생리염수대조조상비차이유통계학의의(P < 0.05);5 d 표체교 48 h 상승,단잉저우생리염수대조조(P < 0.05). 결론:세균감염중추신경계통후,Occludin표체하강차유선하강후증고규률,제시Occludin삼여료감염성뇌수종적발생、발전과정.
Objective To investigate the expressions of Occludin in brain after bacterial meningitis and to discuss possible molecular mechanism of bacterial meningitis when brain edema occurs. Methods The models of bacterial meningitis and normal control were constructed via inoculating intracisternally with strain Ⅲ streptococcus pneumoniae and the same volume of normal saline solution, respectively. The expression of Occludin in brain was detected by immunohistochemistry and Western blot methods respectively and 24 h, 48 h and 5 days after inoculation. Results (1) Loeffler neurologic deficit score (NDS) in 24 h, 48 h and 5 d decreased significantly when compared with that of control group (P < 0.05). (2) After the brain received streptococcus pneumoniae injection, expression of Occludin began to decrease at 24 h and touch the bottom at 48 h,then increase at the 5th day, but still remained lower than that in control group, which indicated statistical difference (P < 0.05). Conclusions Expression of Occludin in the models of bacterial meningitis decreased firstly and then increased regularly. It suggests that Occludin plays a protective role during the development of infectious brain edema.