南方医科大学学报
南方醫科大學學報
남방의과대학학보
Journal of Southern Medical University
2015年
8期
1211-1214,1221
,共5页
卵巢癌%腹腔转移%survivin%反义寡核苷酸%IL-6/STAT3信号通路
卵巢癌%腹腔轉移%survivin%反義寡覈苷痠%IL-6/STAT3信號通路
란소암%복강전이%survivin%반의과핵감산%IL-6/STAT3신호통로
ovarian cancer%peritoneal metastasis%survivin%oligonucleotides%IL-6/STAT3 signaling pathway
目的 探讨survivin反义寡核苷酸(ASODN)在活体内对人卵巢癌细胞种植性转移能力和腹腔移植瘤形成的影响,分析其可能的作用机制.方法 构建人卵巢癌SKOV3细胞株裸鼠腹腔移植瘤模型,实验组给予survivin ASODN腹腔注射,同时对照组给予生理盐水腹腔注射,比较两组动物腹腔移植瘤形成和生长情况的改变,并通过蛋白印迹法检测两组腹腔移植瘤组织中白细胞介素6(IL-6)、信号转导与活化因子3(STAT3)、磷酸化的信号转导与活化因子3(p-STAT3)、survivin蛋白表达的改变.结果 Survivin ASODN组人卵巢癌细胞裸鼠腹腔移植瘤数量和重量都较对照组明显减小(P<0.05),IL-6、STAT3、p-STAT3、survivin蛋白表达均明显下降(P<0.05).结论 Survivin ASODN在活体内能有效降低人卵巢癌细胞侵袭和种植性转移能力,能有效抑制卵巢癌腹腔移植瘤的生长,这些作用是通过抑制IL-6/STAT3信号通路的活性来完成的.靶向survivin基因的反义核苷酸治疗将成为临床防治卵巢癌复发和转移重要手段,有重要的临床价值.
目的 探討survivin反義寡覈苷痠(ASODN)在活體內對人卵巢癌細胞種植性轉移能力和腹腔移植瘤形成的影響,分析其可能的作用機製.方法 構建人卵巢癌SKOV3細胞株裸鼠腹腔移植瘤模型,實驗組給予survivin ASODN腹腔註射,同時對照組給予生理鹽水腹腔註射,比較兩組動物腹腔移植瘤形成和生長情況的改變,併通過蛋白印跡法檢測兩組腹腔移植瘤組織中白細胞介素6(IL-6)、信號轉導與活化因子3(STAT3)、燐痠化的信號轉導與活化因子3(p-STAT3)、survivin蛋白錶達的改變.結果 Survivin ASODN組人卵巢癌細胞裸鼠腹腔移植瘤數量和重量都較對照組明顯減小(P<0.05),IL-6、STAT3、p-STAT3、survivin蛋白錶達均明顯下降(P<0.05).結論 Survivin ASODN在活體內能有效降低人卵巢癌細胞侵襲和種植性轉移能力,能有效抑製卵巢癌腹腔移植瘤的生長,這些作用是通過抑製IL-6/STAT3信號通路的活性來完成的.靶嚮survivin基因的反義覈苷痠治療將成為臨床防治卵巢癌複髮和轉移重要手段,有重要的臨床價值.
목적 탐토survivin반의과핵감산(ASODN)재활체내대인란소암세포충식성전이능력화복강이식류형성적영향,분석기가능적작용궤제.방법 구건인란소암SKOV3세포주라서복강이식류모형,실험조급여survivin ASODN복강주사,동시대조조급여생리염수복강주사,비교량조동물복강이식류형성화생장정황적개변,병통과단백인적법검측량조복강이식류조직중백세포개소6(IL-6)、신호전도여활화인자3(STAT3)、린산화적신호전도여활화인자3(p-STAT3)、survivin단백표체적개변.결과 Survivin ASODN조인란소암세포라서복강이식류수량화중량도교대조조명현감소(P<0.05),IL-6、STAT3、p-STAT3、survivin단백표체균명현하강(P<0.05).결론 Survivin ASODN재활체내능유효강저인란소암세포침습화충식성전이능력,능유효억제란소암복강이식류적생장,저사작용시통과억제IL-6/STAT3신호통로적활성래완성적.파향survivin기인적반의핵감산치료장성위림상방치란소암복발화전이중요수단,유중요적림상개치.
Objective To observe the effect of anti-survivin oligonucleotides (ASODN) on the invasion and growth of peritoneally implanted ovarian cancer cell xenografts in nude mice. Methods Nude mouse models bearing peritoneally implanted ovarian cancer cell (SKOV3) xenografts were established and subjected to intraperitoneal injection of survivin ASODN or saline (control). The number and weight of the intraperitoneal xenografts were compared between the two groups. The expressions of interieukin (IL-6), signal transducer and activator of transcription3 (STAT3), phosphorylated STAT3 (p-STAT3), and survivin protein in the tumor tissues were detected with Western blotting in both groups. Results Compared with those in the control group, the number and weight of the intraperitoneal xenografts were significantly reduced in ASODN group (P<0.05). ASODN treatment also resulted in significantly lowered protein levels of IL-6, STAT3, p-STAT3, and survivin in the tumor tissues (P<0.05). Conclusion Survivin ASODN can suppress the invasion and migration capacity of ovarian cancer cells and inhibit peritoneal metastasis of the tumor in nude mice possibly though down-regulation of IL-6/STAT3 signaling pathway.
