CAJ | 학술논문

目的探讨雷尼酸锶( strontium ranelate,SR)对大鼠股骨骨质疏松性骨折愈合的影响. 方法 6个月龄Wistar雌性处鼠66只,切除双侧卵巢制作骨质疏松动物模型,模型建立成功后,在大鼠股骨中段横行截骨,制作大鼠股骨骨质疏松性骨折动物模型,术后死亡6只,然后将剩余60只大鼠随机分为实验组( SR干预治疗组)和空白对照组,每组30只. 骨折术后第一天起,实验组给予SR 400 mg/( kg· d)灌胃,对照组给予同等体积的生理盐水灌胃. 通过影像学、组织学和免疫组织化学(骨形态发生蛋白-2,BMP-2)观察,骨组织形态计量学、骨密度(bone mineral density,BMD)和生物力学测量,观察骨折愈合情况. 结果实验组腰4、5椎体BMD、BMP-2阳性表达细胞数、骨小梁面积比、骨小梁平均宽度、最大负荷和最大桡度高于同时期对照组,差异有统计学意义( P<0.01);骨小梁平均间隔小于同期对照组,差异有统计学意义( P<0.01). 结论 SR具有抑制骨吸收和促进骨形成的双重作用,能增加BMD,加快骨痂形成,促进骨质疏松性骨折愈合,改善骨的显微结构,提高骨折愈合后骨的生物力学特性.
목적 탐토뢰니산송( strontium ranelate,SR)대대서고골골질소송성골절유합적영향. 방법 6개월령Wistar자성처서66지,절제쌍측란소제작골질소송동물모형,모형건립성공후,재대서고골중단횡행절골,제작대서고골골질소송성골절동물모형,술후사망6지,연후장잉여60지대서수궤분위실험조( SR간예치료조)화공백대조조,매조30지. 골절술후제일천기,실험조급여SR 400 mg/( kg· d)관위,대조조급여동등체적적생리염수관위. 통과영상학、조직학화면역조직화학(골형태발생단백-2,BMP-2)관찰,골조직형태계량학、골밀도(bone mineral density,BMD)화생물역학측량,관찰골절유합정황. 결과실험조요4、5추체BMD、BMP-2양성표체세포수、골소량면적비、골소량평균관도、최대부하화최대뇨도고우동시기대조조,차이유통계학의의( P<0.01);골소량평균간격소우동기대조조,차이유통계학의의( P<0.01). 결론 SR구유억제골흡수화촉진골형성적쌍중작용,능증가BMD,가쾌골가형성,촉진골질소송성골절유합,개선골적현미결구,제고골절유합후골적생물역학특성.
Objective To investigate the effect of strontium ranelate ( SR) on fracture healing of the femurs in osteoporotic rats. Methods Sixty-six 6-month-old female virgin Wistar rats were bilateral ovariectomized and cut the middle shaft of the femurs to create osteoporosis fracture animal model.Six rats died after surgery.The rest 60 rats were randomly divided into experimental group and control group, with 30 rats in each group.From the first day after fracture rats in experimental group received SR 400 mg/( kg· d) by intragastric administration, and rats in control group received the same volume of normal saline.The fracture healing was observed using imaging, histology, immunohistochemistry (bone morphogenetic protein-2, BMP-2), bone mineral density (BMD), and biomechanics.Results BMD of lumbar vertebrae 4 and 5, number of BMP-2 positive cells, trabecular area, trabecular width, and the biomechanical property in experimental group were higher than those in the control group ( P <0.01 ) , and the trabecular separation in the experimental group was smaller than that in the control group (P<0.01).Conclusion SR has inhibiting effect on bone resorption and stimulating effect on bone formation.It can increase BMD, promote the osteoporosis fracture healing, improve the microstructure of the bone, and enhance the biomechanical properties.