中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
Chinese Journal of Osteoporosis
2015年
8期
990-996
,共7页
王玉明%张晓阳%赵宏斌%胡敏%白松%韩雨杉%伍雪%钱传云
王玉明%張曉暘%趙宏斌%鬍敏%白鬆%韓雨杉%伍雪%錢傳雲
왕옥명%장효양%조굉빈%호민%백송%한우삼%오설%전전운
Runx2基因%骨质疏松%骨密度%基因多态性%等位基因
Runx2基因%骨質疏鬆%骨密度%基因多態性%等位基因
Runx2기인%골질소송%골밀도%기인다태성%등위기인
Runx2 gene%Osteoporosis%Bone mineral density%Polymorphism%Allele
目的 比较云南红河地区哈尼族与昆明地区汉族Runx2基因多态性. 方法 将昆明医科大学第一附属医院120名汉族健康体检者作为汉族人群,云南省红河地区126名哈尼族作为哈尼族人群. 应用聚合酶链反应-限制性片断长度多态性( PCR-RFLP)方法分析Runx2基因第一启动子P1-330G/T位点等位基因多态性,应用RT-PCR TaqMan SNP基因分型技术分析Runx2基因第二启动子P2-1025 T/C位点等位基因多态性,并测序验证基因型. 采用超声测量仪测量研究人群右足跟骨的骨密度( BMD). 比较汉族人群和哈尼族人群在基因型分布和等位基因频率之间的差异,分析哈尼族人群BMD与影响因素之间的关系. 结果 Runx2基因P1-330G/T位点GG、GT、TT基因型分布以及G、T等位基因频率,Runx2基因P2-1025T/C位点TT、TC、CC基因型分布以及T、C等位基因频率在云南昆明地区汉族、云南红河地区哈尼族人群间无统计学差异( P>0.05 ).Runx2基因P1-330G/T位点GG和GT基因型,Runx2 基因P2-1025T/C位点TC和TT基因型在哈尼族人群BMD正常和减低组间的差异有统计学意义(P<0.05). Logistic回归分析显示,BMD与哈尼族人群Runx2基因P1-330 G/T位点、P2-1025T/C位点、以及年龄因素的OR值和95%CI分别为:1.337,0.649~2.756;0.132,0.024~0.710;1.101, 1.041~1.163. 结论 云南红河地区哈尼族与昆明地区汉族Runx2基因P1-330G/T位点和P2-1025T/C位点的基因型分布以及等位基因频率无人群间差异和地域性差异. Runx2基因P1-330G/T位点和P2-1025T/C位点的基因型分布以及等位基因频率在云南省红河地区哈尼族人群BMD正常和减低组间存在显著差异. Runx2基因P1-330G/T位点与BMD无关,年龄是BMD的危险因素,Runx2基因P2-1025T/C位点可能是BMD的保护因素,该位点的T等位基因可能是BMD的保护基因.
目的 比較雲南紅河地區哈尼族與昆明地區漢族Runx2基因多態性. 方法 將昆明醫科大學第一附屬醫院120名漢族健康體檢者作為漢族人群,雲南省紅河地區126名哈尼族作為哈尼族人群. 應用聚閤酶鏈反應-限製性片斷長度多態性( PCR-RFLP)方法分析Runx2基因第一啟動子P1-330G/T位點等位基因多態性,應用RT-PCR TaqMan SNP基因分型技術分析Runx2基因第二啟動子P2-1025 T/C位點等位基因多態性,併測序驗證基因型. 採用超聲測量儀測量研究人群右足跟骨的骨密度( BMD). 比較漢族人群和哈尼族人群在基因型分佈和等位基因頻率之間的差異,分析哈尼族人群BMD與影響因素之間的關繫. 結果 Runx2基因P1-330G/T位點GG、GT、TT基因型分佈以及G、T等位基因頻率,Runx2基因P2-1025T/C位點TT、TC、CC基因型分佈以及T、C等位基因頻率在雲南昆明地區漢族、雲南紅河地區哈尼族人群間無統計學差異( P>0.05 ).Runx2基因P1-330G/T位點GG和GT基因型,Runx2 基因P2-1025T/C位點TC和TT基因型在哈尼族人群BMD正常和減低組間的差異有統計學意義(P<0.05). Logistic迴歸分析顯示,BMD與哈尼族人群Runx2基因P1-330 G/T位點、P2-1025T/C位點、以及年齡因素的OR值和95%CI分彆為:1.337,0.649~2.756;0.132,0.024~0.710;1.101, 1.041~1.163. 結論 雲南紅河地區哈尼族與昆明地區漢族Runx2基因P1-330G/T位點和P2-1025T/C位點的基因型分佈以及等位基因頻率無人群間差異和地域性差異. Runx2基因P1-330G/T位點和P2-1025T/C位點的基因型分佈以及等位基因頻率在雲南省紅河地區哈尼族人群BMD正常和減低組間存在顯著差異. Runx2基因P1-330G/T位點與BMD無關,年齡是BMD的危險因素,Runx2基因P2-1025T/C位點可能是BMD的保護因素,該位點的T等位基因可能是BMD的保護基因.
목적 비교운남홍하지구합니족여곤명지구한족Runx2기인다태성. 방법 장곤명의과대학제일부속의원120명한족건강체검자작위한족인군,운남성홍하지구126명합니족작위합니족인군. 응용취합매련반응-한제성편단장도다태성( PCR-RFLP)방법분석Runx2기인제일계동자P1-330G/T위점등위기인다태성,응용RT-PCR TaqMan SNP기인분형기술분석Runx2기인제이계동자P2-1025 T/C위점등위기인다태성,병측서험증기인형. 채용초성측량의측량연구인군우족근골적골밀도( BMD). 비교한족인군화합니족인군재기인형분포화등위기인빈솔지간적차이,분석합니족인군BMD여영향인소지간적관계. 결과 Runx2기인P1-330G/T위점GG、GT、TT기인형분포이급G、T등위기인빈솔,Runx2기인P2-1025T/C위점TT、TC、CC기인형분포이급T、C등위기인빈솔재운남곤명지구한족、운남홍하지구합니족인군간무통계학차이( P>0.05 ).Runx2기인P1-330G/T위점GG화GT기인형,Runx2 기인P2-1025T/C위점TC화TT기인형재합니족인군BMD정상화감저조간적차이유통계학의의(P<0.05). Logistic회귀분석현시,BMD여합니족인군Runx2기인P1-330 G/T위점、P2-1025T/C위점、이급년령인소적OR치화95%CI분별위:1.337,0.649~2.756;0.132,0.024~0.710;1.101, 1.041~1.163. 결론 운남홍하지구합니족여곤명지구한족Runx2기인P1-330G/T위점화P2-1025T/C위점적기인형분포이급등위기인빈솔무인군간차이화지역성차이. Runx2기인P1-330G/T위점화P2-1025T/C위점적기인형분포이급등위기인빈솔재운남성홍하지구합니족인군BMD정상화감저조간존재현저차이. Runx2기인P1-330G/T위점여BMD무관,년령시BMD적위험인소,Runx2기인P2-1025T/C위점가능시BMD적보호인소,해위점적T등위기인가능시BMD적보호기인.
Objective To explore the polymorphism of Runx2 gene between Hani ethnic minority in Honghe and Han nationality in Kunming, Yunnan.Methods One hundred and twenty healthy screening people in the First Affiliated Hospital of Kunming Medical University were as Han population, and 126 Hani people in Honghe region were as Hani population.The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to analyze the Runx2 gene promoter P1-330G/T allele polymorphism site.The RT-PCR TaqMan SNP genotyping method was used to analyze Runx2 gene second promoter P2-1025T/C allele polymorphism site, and the genotypes were sequenced.The bone mineral density (BMD) of the right calcaneus was measured using ultrasonic instrument.The difference of genotype distribution and allele frequencies between Hani ethnic minority and Han populations was compared, and the relationship between influencing factors and BMD in Hani population was analyzed. Results Both the genotype distribution sites of GG, GT, TT of Runx2 gene P1-330G/T and G, T allele frequency, and the genotype distribution sites of TT, TC, CC of Runx2 gene P2-1025T/C and T, C allele frequency had no significant difference between Hani ethnic minority and Han population in Yunnan (P>0.05).The genotype sites like GG and GT of Runx2 gene P1-1025T/C, and genotype sites like TC and TT of Runx2 gene P2-1025T/C were different between normal BMD and low BMD group in Hani ethnic minority ( P<0.05 ) .Logistic regression analysis showed that in Hani population, the OR and 95% CI of Runx2 gene P1-330 G/T locus, P2-1025T/C locus, and age to BMD were 1.337, 0.649-2.756, 0.132, 0.024-0.710, and 1.101, 1.041-1.163, respectively.Conclusion The genotype distribution and allele frequencies of Runx2 gene P1-330 G/T and P2-1025 T/C polymorphism sites had no population and regional differences.The genotype distribution and allele frequencies of Runx2 gene P1-330G/T and P2-1025G/T polymorphism sites show significant difference between normal BMD and low BMD group of Hani population in Yunnan.Runx2 gene P1-330G/T site is not associated with BMD.Age is a risk factor for BMD.Runx2 gene P2-1025T/C site may be a protection factor of BMD, and the T allele gene of this site may be a protective allele of BMD.