中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
Chinese Journal of Osteoporosis
2015年
8期
971-976,985
,共7页
谢丽华%范超领%李生强%陈娟%许惠娟%葛继荣
謝麗華%範超領%李生彊%陳娟%許惠娟%葛繼榮
사려화%범초령%리생강%진연%허혜연%갈계영
绝经后骨质疏松症%肾阴虚证%六味地黄丸%基因芯片%中医
絕經後骨質疏鬆癥%腎陰虛證%六味地黃汍%基因芯片%中醫
절경후골질소송증%신음허증%륙미지황환%기인심편%중의
Postmenopausal osteoporosis%Kidney Yin deficiency%Liuwei Dihuang Pills%Microarray%Traditional Chinese medicine
目的 探讨六味地黄丸对绝经后骨质疏松症肾阴虚证( POP)差异表达基因的影响. 方法 用基因芯片技术比较POP肾阴虚证组与POP肾阳虚证组、POP无肾虚证组、健康对照组受试者外周血基因表达谱,筛选出差异表达基因;六味地黄丸治疗POP肾阴虚证组3个月后,用基因芯片检测六味地黄丸对这些差异表达基因的影响. 结果 ①基因芯片筛选POP肾阴虚证组与其他3组比较均有显著性差异的差异表达基因:ASB1、CLCF1、PROK2、GPR27、C3orf35、GSTM5、MUC12,其中ASB1、CLCF1、PROK2、GPR27和C3orf35表达下调,GSTM5和MUC12表达上调. ②六味地黄丸治疗POP肾阴虚证组3个月后,基因芯片检测ASB1、CLCF1的表达显著上调, CLCF1参与JAK-STAT信号通路中JAK1、CBP显著下调、STAT4显著上调,而PROK2、GPR27、C3orf35和GSTM5表达水平治疗前后比较无显著性差异. ③六味地黄丸治疗POP肾阴虚证组3个月后,与治疗前相比,共有5701个差异表达基因,其中上调基因3072个,下调基因2629个. 结论 六味地黄丸上调POP肾阴虚证差异基因ASB1、CLCF1的表达,其治疗POP肾阴虚证的机理可能与其上调CLCF1介导JAK-STAT信号通路调控下游CBP表达有关.
目的 探討六味地黃汍對絕經後骨質疏鬆癥腎陰虛證( POP)差異錶達基因的影響. 方法 用基因芯片技術比較POP腎陰虛證組與POP腎暘虛證組、POP無腎虛證組、健康對照組受試者外週血基因錶達譜,篩選齣差異錶達基因;六味地黃汍治療POP腎陰虛證組3箇月後,用基因芯片檢測六味地黃汍對這些差異錶達基因的影響. 結果 ①基因芯片篩選POP腎陰虛證組與其他3組比較均有顯著性差異的差異錶達基因:ASB1、CLCF1、PROK2、GPR27、C3orf35、GSTM5、MUC12,其中ASB1、CLCF1、PROK2、GPR27和C3orf35錶達下調,GSTM5和MUC12錶達上調. ②六味地黃汍治療POP腎陰虛證組3箇月後,基因芯片檢測ASB1、CLCF1的錶達顯著上調, CLCF1參與JAK-STAT信號通路中JAK1、CBP顯著下調、STAT4顯著上調,而PROK2、GPR27、C3orf35和GSTM5錶達水平治療前後比較無顯著性差異. ③六味地黃汍治療POP腎陰虛證組3箇月後,與治療前相比,共有5701箇差異錶達基因,其中上調基因3072箇,下調基因2629箇. 結論 六味地黃汍上調POP腎陰虛證差異基因ASB1、CLCF1的錶達,其治療POP腎陰虛證的機理可能與其上調CLCF1介導JAK-STAT信號通路調控下遊CBP錶達有關.
목적 탐토륙미지황환대절경후골질소송증신음허증( POP)차이표체기인적영향. 방법 용기인심편기술비교POP신음허증조여POP신양허증조、POP무신허증조、건강대조조수시자외주혈기인표체보,사선출차이표체기인;륙미지황환치료POP신음허증조3개월후,용기인심편검측륙미지황환대저사차이표체기인적영향. 결과 ①기인심편사선POP신음허증조여기타3조비교균유현저성차이적차이표체기인:ASB1、CLCF1、PROK2、GPR27、C3orf35、GSTM5、MUC12,기중ASB1、CLCF1、PROK2、GPR27화C3orf35표체하조,GSTM5화MUC12표체상조. ②륙미지황환치료POP신음허증조3개월후,기인심편검측ASB1、CLCF1적표체현저상조, CLCF1삼여JAK-STAT신호통로중JAK1、CBP현저하조、STAT4현저상조,이PROK2、GPR27、C3orf35화GSTM5표체수평치료전후비교무현저성차이. ③륙미지황환치료POP신음허증조3개월후,여치료전상비,공유5701개차이표체기인,기중상조기인3072개,하조기인2629개. 결론 륙미지황환상조POP신음허증차이기인ASB1、CLCF1적표체,기치료POP신음허증적궤리가능여기상조CLCF1개도JAK-STAT신호통로조공하유CBP표체유관.
Objective To study the influence of Liuwei Dihuang Pills ( LDP) on differentially expressed genes in kidney Yin deficiency patients with postmenopausal osteoporosis ( POP ) .Methods Microarray was used to identify the differentially expressed genes in patients with POP kidney Yin deficiency, with kidney Yang deficiency, with no Yin deficiency, and healthy controls, in the peripheral blood.These genes were further analyzed through examination of differential gene expression patterns before and after LDP treatment.Results (1) In patients with POP kidney Yin deficiency, ASB1, CLCF1, PROK2, GPR27, and C3orf35 were down-regulated, and GSTM5 and MUC12 were up-regulated, comparing to other 3 groups.(2) After LDP treatment for 3 months, ASB1 and CLCF1 were significantly up-regulated, JAK1 and CBP were significantly down-regulated, and PROK2, GPR27, C3orf35, and GSTM5 were not change.(3) After 3-month treatment, there were 5701 differentially expressed genes, including 3072 up-regulated genes and 2629 down-regulated genes.Conclusion LDP significantly increases the expression of ASB1 and CLCF1.The potential mechanism underlying the therapeutic effect of LDP on POP kidney Yin deficiency may be related to regulation of CBP in JAK-STAT signaling pathway.
