实用医药杂志
實用醫藥雜誌
실용의약잡지
Practical Journal of Medicine & Pharmacy
2015年
8期
741-744
,共4页
黄连%吴茱萸%配伍比例%结肠癌细胞
黃連%吳茱萸%配伍比例%結腸癌細胞
황련%오수유%배오비례%결장암세포
Coptidis Rhizoma%Evodiae Fructus%Proportion%Colon cancer cell
目的 比较黄连、吴茱萸不同配比的水提物对结肠癌细胞CT-26增殖、迁移和凋亡的影响,从而找出两药的最佳配比.方法 采用MTT试验、克隆形成试验、细胞划痕试验、流式细胞术观察不同配比(黄连:吴茱萸比例为1:6、2:5、3:4、4:3、5:2、6:1以及两药单独作用)的水提物对CT-26细胞增殖、迁移和凋亡的影响. 结果 黄连、5:2、6:1组对细胞增殖的抑制作用明显,6:1组作用6 h后即产生抑制作用,12、24、48 h的细胞增殖抑制率均大于5:2组和黄连组,且差异有统计学意义(P<0.01),6:1、5:2 和黄连组作用48 h后的增殖抑制率分别达到(80.39± 2.72)%、(56.34±12.57)%、(62.71±5.46)%,且黄连、吴茱萸配伍对细胞增殖的抑制作用存在时间依赖性和剂量依赖性. 随着配比中黄连比例的增加,克隆形成率逐渐降低,2:5、3:4、4:3、5:2、6:1、黄连组的集落形成率与空白对照组相比差异均有统计学意义(P<0.01). 划痕实验结果表明黄连组和6:1组作用24 h后,与空白对照组相比能够明显抑制划痕愈合的程度.流式细胞仪PI单染亚二倍体峰分析结果显示,随着配比中黄连比例的增加,细胞凋亡率上升,作用24 h后黄连和6:1 组的细胞凋亡率分别达到(31.05±1.34)%、(35.09±3.72)%,且两者差异有统计学意义(P<0.05). 结论 黄连、吴茱萸配伍对结肠癌细胞CT-26的增殖、迁移和凋亡均有一定的抑制作用,其中以配比为6:1时作用最显著.
目的 比較黃連、吳茱萸不同配比的水提物對結腸癌細胞CT-26增殖、遷移和凋亡的影響,從而找齣兩藥的最佳配比.方法 採用MTT試驗、剋隆形成試驗、細胞劃痕試驗、流式細胞術觀察不同配比(黃連:吳茱萸比例為1:6、2:5、3:4、4:3、5:2、6:1以及兩藥單獨作用)的水提物對CT-26細胞增殖、遷移和凋亡的影響. 結果 黃連、5:2、6:1組對細胞增殖的抑製作用明顯,6:1組作用6 h後即產生抑製作用,12、24、48 h的細胞增殖抑製率均大于5:2組和黃連組,且差異有統計學意義(P<0.01),6:1、5:2 和黃連組作用48 h後的增殖抑製率分彆達到(80.39± 2.72)%、(56.34±12.57)%、(62.71±5.46)%,且黃連、吳茱萸配伍對細胞增殖的抑製作用存在時間依賴性和劑量依賴性. 隨著配比中黃連比例的增加,剋隆形成率逐漸降低,2:5、3:4、4:3、5:2、6:1、黃連組的集落形成率與空白對照組相比差異均有統計學意義(P<0.01). 劃痕實驗結果錶明黃連組和6:1組作用24 h後,與空白對照組相比能夠明顯抑製劃痕愈閤的程度.流式細胞儀PI單染亞二倍體峰分析結果顯示,隨著配比中黃連比例的增加,細胞凋亡率上升,作用24 h後黃連和6:1 組的細胞凋亡率分彆達到(31.05±1.34)%、(35.09±3.72)%,且兩者差異有統計學意義(P<0.05). 結論 黃連、吳茱萸配伍對結腸癌細胞CT-26的增殖、遷移和凋亡均有一定的抑製作用,其中以配比為6:1時作用最顯著.
목적 비교황련、오수유불동배비적수제물대결장암세포CT-26증식、천이화조망적영향,종이조출량약적최가배비.방법 채용MTT시험、극륭형성시험、세포화흔시험、류식세포술관찰불동배비(황련:오수유비례위1:6、2:5、3:4、4:3、5:2、6:1이급량약단독작용)적수제물대CT-26세포증식、천이화조망적영향. 결과 황련、5:2、6:1조대세포증식적억제작용명현,6:1조작용6 h후즉산생억제작용,12、24、48 h적세포증식억제솔균대우5:2조화황련조,차차이유통계학의의(P<0.01),6:1、5:2 화황련조작용48 h후적증식억제솔분별체도(80.39± 2.72)%、(56.34±12.57)%、(62.71±5.46)%,차황련、오수유배오대세포증식적억제작용존재시간의뢰성화제량의뢰성. 수착배비중황련비례적증가,극륭형성솔축점강저,2:5、3:4、4:3、5:2、6:1、황련조적집락형성솔여공백대조조상비차이균유통계학의의(P<0.01). 화흔실험결과표명황련조화6:1조작용24 h후,여공백대조조상비능구명현억제화흔유합적정도.류식세포의PI단염아이배체봉분석결과현시,수착배비중황련비례적증가,세포조망솔상승,작용24 h후황련화6:1 조적세포조망솔분별체도(31.05±1.34)%、(35.09±3.72)%,차량자차이유통계학의의(P<0.05). 결론 황련、오수유배오대결장암세포CT-26적증식、천이화조망균유일정적억제작용,기중이배비위6:1시작용최현저.
Objective To investigate the effect of water extracts of Coptidis Rhizoma and Evodiae Fructus at different ratios on the proliferation,migration and apoptosis of colon cancer cell CT-26 and to determine the optimal proportion of both. Methods Using MTT assay,clone formation assay,the wound healing and flow cytometry the proliferation,migration and apoptosis ability of CT-26 cells in experimental groups (Coptidis Rhizoma:Evodiae Fructus ratios at 1:6、2:5、3:4、4:3、5:2、6:1 and single of the two medicines) were detected. Results Coptidis Rhizoma,5:2,6:1 groups showed a remarkable inhibition effect on CT-26 ability of proliferation. After a 6h treatment of 6:1 group,there was an obvious inhibition of cell growth and it was significantly higher than that of 5:2 and Coptidis Rhizoma group (P<0.01). After 48h treatment of 6:1,5:2 and optidis Rhizoma groups,the proliferation inhibition rates reached (80.39±2.72)%,(56.34±12.57)% and (62.71±5.46)%,respectively. In addition,the growth inhibition effect exhibited a time-dependent and dose-dependent pattern. As the proportion of Coptidis Rhizoma in experiment groups increased,the clone formation rate decreased gradually and in 2:5,3:4,4:3,5:2,6:1 and Coptidis Rhizoma groups the rates were lower significantly than that in the control group (P<0.01). The wound healing indicated that Coptidis Rhizoma and 6:1 groups' 48h treatment could obviously inhibite the wound healing process. Flow cytometry analysis showed that with the increase of Coptidis Rhizoma the apoptosis rates increased and after 24h treatment of Coptidis Rhizoma and 6:1 groups the rates were (31.05± 1.34)%, (35.09 ±3.72)%(P<0.05). Conclusion The combination of Coptidis Rhizoma and Evodiae Fructus can inhibit the proliferation,migration and induce apoptosis of CT-26 cells,and the strongest effect is achieved at the proportion of Coptidis Rhizoma and vodiae Fructus at 6:1.
