CAJ | 학술논문

目的通过对2012年深圳市鼻病毒基因特性及分子变异的分析,初步了解深圳市鼻病毒流行及基因变异情况. 方法荧光RT-PCR对门诊采集的流感样病例样本进行筛查,通过巢式RT-PCR对其VP4~VP2片段和VP1基因进行扩增,利用Clustal W和MEGA等软件进行分子变异分析. 结果 2012年深圳市有A、B两个型别的鼻病毒同时流行,其中A型占多数,包括A47、A31、A90、A18等亚型,其中出现了两株A47和A31的基因重组病毒;VP1分子的变异具有型间差异,且变异位点散乱;不同亚型的受体结合区( BC、DE和HI环)位点并不一致,具有多态性;25个药敏位点中有5个位点(I121V、L123M、V167I、Y189H、H259G)出现了变异. 结论多个亚型的鼻病毒在深圳存在广泛的流行,而且仍在不断的发生重组和变异.
목적 통과대2012년심수시비병독기인특성급분자변이적분석,초보료해심수시비병독류행급기인변이정황. 방법 형광RT-PCR대문진채집적류감양병례양본진행사사,통과소식RT-PCR대기VP4~VP2편단화VP1기인진행확증,이용Clustal W화MEGA등연건진행분자변이분석. 결과 2012년심수시유A、B량개형별적비병독동시류행,기중A형점다수,포괄A47、A31、A90、A18등아형,기중출현료량주A47화A31적기인중조병독;VP1분자적변이구유형간차이,차변이위점산란;불동아형적수체결합구( BC、DE화HI배)위점병불일치,구유다태성;25개약민위점중유5개위점(I121V、L123M、V167I、Y189H、H259G)출현료변이. 결론 다개아형적비병독재심수존재엄범적류행,이차잉재불단적발생중조화변이.
Objective To analyze the genetic characteristics and molecular variation of human rhi-novirus strains isolated in Shenzhen.Methods RNA samples were extracted from nasopharyngeal swab samples collected from influenza-like subjects in Shenzhen and analyzed by fluorescent RT-PCR.The VP4-VP2 and VP1 gene regions of human rhinovirus strains were amplified by nested RT-PCR.Clustal W and MEGA programs were used to evaluate molecular variation of the human rhinovirus strains.Results Both human rhinovirus A and B were prevalent in Shenzhen during 2012.Human rhinoviruses A was the predomi-nant pathogen, including subtypes A47, A31, A90, A18 and so on.Two recombinant strains of human rhi-noviruses A47 and A31 were detected.The mutations scattered on the VP1 protein and varied in different subtypes.The receptor binding sites ( loop BC, DE and HI) in different subtypes showed polymorphism. Five out of twenty-five drug sensitivity sites ( I121V, L123M, V167I, Y189H and H259G) showed muta-tion.Conclusion Multiple subtypes of human rhinovirus were prevalent in Shenzhen and were in a state of constant recombination and variation.